Screening of ammonia-degrading bacteria to reduce ammonia content in the manure of laying hens

Abstract

This study aimed to screen probiotic candidates for the capacity to degrade ammonia content in the manure of laying hens. Seven bacteria previously isolated from broilers’ intestine were assigned to a completely randomized design. A fresh culture of each bacterial isolate was cultured to reach optical density at OD600:0.5. One ml suspension was inoculated into 100 ml samples (2g manure diluted in 100 ml of sterilized distilled water) and incubated for three days. Ammonia concentrations in each sample were measured daily using Ammonium/Ammonia-Test sera ammonia kits. The results showed that the ammonia concentration in all bacteria-treated samples was significantly lower than ammonia content in control, P<0.05). The average amount of ammonia in the control was 1.00±0.44 mg/l, while isolate I1-treated sample was 0.03±0.00 mg/l, isolate I2 0.02±0.00 mg/l, isolate I3 0.02±0.00 mg/l, isolate I4 0.04±0.01 mg/l, isolate I5 0.04±0.01 mg/l, isolate I6 0.02±0.01 mg/l and isolate I7 0.05±0.00 mg/l. The best three ammonia degrading capacity among the seven isolates were I2, I3, and I6. Based on the phenotypical characteristics, these bacteria were identified as Nitrosomonas sp. (I2), Nitrosobolus sp. (I3), and Nitrosococcus sp. (I6). Therefore, these three bacteria were recommended for probiotic candidates in laying hen.

Isolation and screening of indigenous nitrifying bacteria to enhance nutrient recovery in an aquaponics system

“…Representative colonies in terms of color, morphology, and size were purified and put in 15% glycerol stock and placed at −20°C until further use (Amin et al, 2017). Twelve isolates (6 ammonia‐degrading bacteria and 6 nitrite‐degrading bacteria) were further compared according to a protocol previously described by Ali et al (2020) to screen the best ammonia or nitrite‐degrading isolates.…”

Cultivation of Phytase-Producing Bacteria as Probiotic Candidate on Molasses and Tempe-Processing Waste

“…Bacterial isolates that have been cultured are characterized again by observing the morphological form of bacteria macroscopically (morphologically) and microscopic (Gram painting method) [22].…”

Penggunaan Sinbiotik Untuk Meningkatkan Pertumbuhan Ayam Pedaging Di Peternak Muara Selayar Desa Pijot Kabupaten Lombok Timur

“…Sinbiotik merupakan campuran antara prebiotik (bahan pakan yang sulit tercerna di saluran pencernaan namun menguntungkan inang melalui stimulasi selektif pertumbuhan atau aktifitas satu atau beberapa mikroba di usus) dengan probiotik (mikroba hidup yang memiliki manfaat untuk inang) 7,8 . Penggunaan sinbiotik telah berhasil meningkatkan daya tahan tubuh dan pertumbuhan ayam potong 9 maupun ayam petelur 10,11 .…”

“…Beberapa isolat bakteri probiotik yang dapat memecah serat dan komplek fitat pada pakan serta dapat mereduksi amonia telah dihasilkan di Universitas Mataram 10,11 . Pemecahan komplek fitat tersebut sangat penting untuk melepas mineral mahal dan sangat penting "posfor" sehingga menjadi dapat dimanfaatkan oleh ayam pedaging 12 .…”