Screening of Algerian Medicinal Plants for Acetylcholinesterase Inhibitory Activity

Benamar, Houari; Rached, Wahiba; Derdour, Aïcha; Marouf, Abderrazak

doi:10.3923/jbs.2010.1.9

Cited by 57 publications

(39 citation statements)

References 27 publications

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“…More so, the butyrylcholinesterase BChE inhibition by the essential oils is also beneficial as BChE increases the neurotoxicity of certain plaques thereby making the brain more susceptible to Alzheimer s disease 33,34 . The anticholinesterase activity of the essential oil could be linked to the phenolic content, as some phenolic rich plant extracts have been shown to inhibit cholinesterases 35 . The anticholinesterase property of the essential oil was lower than that of prostigmine as shown in Fig.…”

Section: Anticholinesterase Activitymentioning

confidence: 99%

Essential Oil from Lemon Peels Inhibit Key Enzymes Linked to Neurodegenerative Conditions and Pro-oxidant Induced Lipid Peroxidation

2014

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Section: Anticholinesterase Activitymentioning

confidence: 99%

Essential Oil from Lemon Peels Inhibit Key Enzymes Linked to Neurodegenerative Conditions and Pro-oxidant Induced Lipid Peroxidation

2014

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“…The results were compared with antioxidant standards. This work lies within the scope of continuation of the published works describing some of biologically active extracts from Algerian Z. lotus [19,20] . To the best of our knowledge, the antioxidant properties of the extracts from Z. lotus using the β-Carotene Bleaching method (BCB) and Total Antioxidant Capacity test (TAC) have not been studied.…”

Section: Introductionmentioning

confidence: 99%

Study on the antioxidant activities of root extracts of Zizyphus lotus from the western region of Algeria

Ghalem¹,

Merghache²,

Belarbi³

2014

Phcog J.

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Objective: To find a new natural source of antioxidant, the phenolic contents and antioxidant activities of some secondary metabolites extracted from roots of Ziziphus lotus were evaluated. Methods: The total polyphenol contents were determined spectrophotometrically. Pyrrocatechol, catechin and cyanidin equivalents were used for these parameters. The antioxidant activities of the extracts were determined by several in vitro systems of assays, namely DPPH radical scavenging activity method, b-Carotene Bleaching assay (BCB), Ferric Reducing Antioxidant Power assay (FRAP) and Total Antioxidant capacity test (TAC). Results: The quantitative estimation showed that the roots of Z. lotus were rich in polyphenols (20.09 mg PE/g DW) and proanthocyannidins (1.56%) and they contained a small amount of flavonoids (0.02 mg CE/g DW). The most fractions exhibited high antioxidant activities, and some even showed higher potency than the standard synthetic antioxidants in some instances. In DPPH assay, all extracts had shown significant inhibition (58.535 -94.730% at 1 mg/mL). In addition, the IC 50 values ranged from 0.211 to 0.816 mg/mL, compared to 0.110, 0.214, 0.214 and 0.413 mg/mL for gallic acid, tannic acid, butylated-hydroxyanisol and ascorbic acid respectively. In (BCB) assay, the extracts showed strong inhibition (55.55 -100.00% at 1 mg/mL) and the IC 50 values ranging from 0.123 to 0.850 mg/mL compared to 0.433 mg/mL for gallic acid. A dose dependant curve was obtained for all extracts in the FRAP assay. However, the antioxidant potencies of ascorbic acid and extracts were comparable at low concentrations. The majority of extracts showed the highest value of antioxidant activity, based on TAC test (0.073 -0.398 mg ascorbic acid / mg extract). Conclusion: The results indicate that Z. lotus roots could be an important sources of natural antioxidants.

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“…The genus Atriplex, commonly known as saltbush or orache, consists of mostly halophytic species with edible property such as AL. To our knowledge, cholinesterase or TYR inhibitory activity of AL has not been studied so far, whereas another species, Atriplex halimus L., was demonstrated to have a significant AChE inhibition by Benamar et al [20]. Particularly, its chloroform extract had 74.60 ± 1.45 % of inhibition at 125 μg mL -1 , while the crude extract of the plant exhibited 66.03 ± 1.10 % of inhibition at 250 μg mL -1 which was shown to contain a very low amount of flavonoids consistent with our data.…”

Section: Total Phenol and Flavonoid Contents And Hplc Analyses Of Thementioning

confidence: 99%

Neurobiological evaluation of thirty-one medicinal plant extracts using microtiter enzyme assays

et al. 2016

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Background: Inhibition of acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) related to Alzheimer's disease as well as tyrosinase (TYR) relevant to Parkinson's disease is an important approach to find novel drug candidates for these diseases. Methods: The extracts from fourteen plant species in various polarities were subjected to high-throughput screening against acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and tyrosinase (TYR), the key enzymes related to Alzheimer's and Parkinson's diseases. The extracts were subjected to the microtiter enzyme inhibition assays at 100 μg mL -1 . Antioxidant effect of the extracts was tested for their scavenging activity against DPPH, DMPD, and NO radicals as well as their ferric-(FRAP) and phosphomolibdenum-reducing power (PRAP) and metalchelation capacity. Total phenol and flavonoid quantities in the extracts were determined spectrophotometrically. HPLC analysis was performed on Atriplex lasiantha, Conringia grandiflora, and Vaccaria hispanica.

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Screening of Algerian Medicinal Plants for Acetylcholinesterase Inhibitory Activity

Cited by 57 publications

References 27 publications

Essential Oil from Lemon Peels Inhibit Key Enzymes Linked to Neurodegenerative Conditions and Pro-oxidant Induced Lipid Peroxidation

Essential Oil from Lemon Peels Inhibit Key Enzymes Linked to Neurodegenerative Conditions and Pro-oxidant Induced Lipid Peroxidation

Study on the antioxidant activities of root extracts of Zizyphus lotus from the western region of Algeria

Neurobiological evaluation of thirty-one medicinal plant extracts using microtiter enzyme assays

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