Screening of Acit Sulphate Soils Fungi From South Kalimantan as Source of Extracelular Enzymes

Abstract

Kalimantan acid sulphate land has the potential to be developed into productive land, with good land optimization. Utilization of rhizosphere microorganism diversity, especially mold can potentially provide a solution in optimizing agricultural land, namely the ability to produce extracellular enzymes. This study aims to determine the potential of mold originating from acid sulphate fields in producing extracellular enzymes (pectinase, chitinase, glucanase, cellulase, and phosphatase). The study was conducted in June-July 2019 at the Microbiology Laboratory, Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development. Screening of extracellular enzyme-producing fungi was carried out on selection media. The results obtained by some isolates have the ability to produce extracellular enzymes. Indications of the ability of mold to produce extracellular enzymes are the presence of clear zones in the selection medium. In pectinase, chitinase and glucanase testing all isolates showed negative results. Potential isolates in producing extracellular enzymes include Penicillium sp. Paddy 4.1 (cellulolytic index 2.43), Clonostachys sp. KRMT 17.9 and Penicillium singorense KLK 13.7 (proteolytic indices 3.97 and 3,00, respectively). The difference in index values ​​indicates the variation in the level of enzyme activity.

The role of rhizosphere microbes as phosphate solubilizing bio fertilizers in shallot: a review

“…Penicillium singorense and Clonostachys sp. are phosphate solubilizers isolated from acid sulfate soils in South Kalimantan, Indonesia [73]. Fungi are stronger phosphate solubilizers than bacteria and make up 0.1-0.5% of the total microbial population, whereas phosphate-solubilizing bacteria make up 1-50% [67,74].…”

Isolasi dan Karakterisasi Jamur Selulolitik pada Fermetodege: Pakan Fermentasi Berbahan Campuran Eceng Gondok, Bekatul Padi, dan Tongkol Jagung

“…Isolat jamur yang menunjukkan kemampuan dalam mendegradasi selulosa ditunjukkan dengan terbentuknya zona bening pada media CMC selanjutnya dilakukan proses karakterisasi berdasarkan kenampakan morfologi makroskopis dan mikroskopis isolat jamur. Pengamatan maskroskopis isolat jamur meliputi pengamatan bentuk koloni, warna koloni pada bagian permukaan (above) dan balik koloni (reverse), tekstur permukaan, tipe miselium, elevasi, dan diameter koloni (Susilowati et al, 2020). Pengamatan mikroskopis isolat jamur selulolitik dilakukan dengan metode slide culture (Valencia dan Meitiniarti, 2017).…”