Screening of 42 medicinal plants for in vivo anthelmintic activity against Dactylogyrus intermedius (Monogenea) in goldfish (Carassius auratus)

Abstract

In the present study, methanol extracts of 42 traditional medicinal plants with potent anthelmintic activity against Dactylogyrus intermedius (Monogenea) in goldfish (Carassius auratus) were investigated. Cinnamomum cassia, Lindera aggregata, and Pseudolarix kaempferi exhibited 100% activity and were selected for further evaluation by applying five solvents (petroleum ether, chloroform, ethyl acetate, methanol, and water) for the extraction of the samples, followed by the in vivo bioassay. Among the extracts tested, water and methanol extracts of C. cassia showed the highest efficacies with EC(50) values of 13.2 and 12.3 mg L(-1), showing 100% efficacy against D. intermedius at 30.0 and 40.0 mg L(-1), followed by methanol extract of L. aggregata which demonstrated 100% efficacy at 60.0 mg L(-1) with EC(50) value of 17.1 mg L(-1) after 48 h of exposure. Methanol and ethyl acetate extract of P. kaempferi, which exhibited a 100% efficacy against D. intermedius at 60.0 and 50.0 mg L(-1), revealed similar activity with EC(50) values of 23.5 and 23.3 mg L(-1), respectively. Acute toxicity of these active extracts was investigated on goldfish for 48 h and the corresponding median lethal concentrations (LC(50)) of 56.9, 31.3, 88.7, 168.2, and 165.7 mg L(-1), respectively. These findings indicated that these extracts of the three plants can be developed as preferred natural antiparasitic agents for the treatment of D. intermedius.

Screening Medicinal Plants for Use against Dactylogyrus intermedius (Monogenea) Infection in Goldfish

“…Therefore, it is logical to look for novel aquaculture medicines that are both environmentally friendly and highly efficient. Extracts from medicinal plants that could offer possible alternatives to control D. intermedius infection have been reported (Liu et al 2010;Wu et al 2011;Ji et al 2012;Lu et al 2012). In the present study, 24 medicinal plants were evaluated for the in vivo anthelmintic activity against D. intermedius (Monogenea) in Goldfish.…”

“…An in vivo study was designed to determine the anthelmintic activity of extracts to D. intermedius according to the previous method of Ji et al (2012). Five fish previously infected with the parasite were placed in plastic basins (N = 120) of 5 L capacity that were filled with 2 L of aerated groundwater.…”

“…Recently, medicinal plants and their extracts were tested for their ability to control D. intermedius, and different extracts from several medicinal plants had significant killing effects in vivo (Wang et al 2009(Wang et al , 2010a(Wang et al , 2010bLiu et al 2010;Wu et al 2011;Ji et al 2012;Lu et al 2012). In our research, we exploited the crude extracts of different medicinal plants extracted by five solvents (petroleum ether, chloroform, ethyl acetate, methanol, and water) for anthelmintic activity against D. intermedius in Goldfish Carassius auratus.…”

Linalool, derived from Cinnamomum camphora (L.) Presl leaf extracts, possesses molluscicidal activity against Oncomelania hupensis and inhibits infection of Schistosoma japonicum

“…There is an increasing awareness of the potential of natural products from plants, which may lead to the development of much-needed new anti-parasitic drugs, with interesting biological activities of cytotoxic and anti-parasitic properties [22–25]. Cinnamomum extracts have been identified as preferred natural antiparasitic agents for the treatment of ectoparasitic louse [26] and mite [27], entoparasites of Dactylogyrus intermedius (a parasitic helminth) [28], Tetratrichomonas gallinarum and Histomonas meleagridis (protozoan parasites) [29], and malaria vector of Anopheles gambiae [30]. No systematic evaluation of molluscicidal or cercaricidal activity of C. camphora extracts against O. hupensis or S. japonicium has been carried out.…”

Aktivitas Anticestoda In Vitro Metabolit Sekunder Daun Miana (Coleus blumei. Benth) terhadap Cacing Hymenolepis microstoma

“…Senyawa Artemisinin aktif terhadap protoscolex dan metacestoda Echinococcus (Spicher et al 2008). Tanaman yang kaya tanin mampu menurunkan jumlah telur per gram (TPG) feses dan jumlah cacing terutama nematoda (Ji et al 2012).…”