Screening, nutritional optimization and purification for phytase produced by Enterobacter aerogenes and its role in enhancement of hydrocarbons degradation and biofilm inhibition

Muslim, Sahira Nsayef; Ali, Alaa Naseer Mohammed; Al-Kadmy, Israa M.S.; Khazaal, Saba Saadoon; Ibrahim, S. A.; Al-Saryi, Nadal; Alsaadi, Luma G.; Muslim, Sraa Nsayef; Salman, Batool Kadham; Aziz, Sarah Naji

doi:10.1016/j.micpath.2017.12.047

Cited by 18 publications

(8 citation statements)

References 21 publications

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“…However, we observed a continuous increase in specific activity of the enzymes from 2.5 FTU/mL to 8.6 U/mL for ammonium sulphate fraction and 17U/mL for gel filtration fraction (Table 2). These values were higher when compared with the specific phytase activity of crude extract (1.13 U/mL), microfiltration fraction (1.15 U/mL), ultrafiltration fraction (1.93 U/mL) and DEAE Sepharose fraction (8.38 U/mL) reported by Muslim et al 1 when solid state fermentation (SSF) was employed. This confirms the limitations of SSF in commercial enzyme production as previously highlighted by 6 .…”

Section: Resultsmentioning

confidence: 73%

“…Phytase (EC 3.1.3.8) is an enzyme capable of hydrolysing phytic acid (myo-inositol hexakisphosphate) -an indigestible form of phosphorus that is found in grains and oil seeds. 1 Two broad groups of phytase known based on optimum pH requirements are the histidine acid phosphatase and alkaline phytase. The first group has an optimum pH around 5.0 while the second have an optimum pH around 8.0.…”

Section: Introductionmentioning

confidence: 99%

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Purification and Characterization of Phytase Produced by Aspergillus niger Isolated from Poultry Farm Soil Using Submerged Fermentation

2021

TJNPR

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Phytase is used in feed processing as an exogenous supplement in monogastric animal feed. This study was aimed at determining phytase production capacity of Aspergillus niger isolated from poultry farm soil. A. niger was isolated from poultry farm soil using phytase screening medium (PSM) containing sodium phytate as substrate. Quantitative estimation of phytase production was carried out by submerged fermentation using 3 x 10 5 spores/100 mL PSM for 5 days at 30 o C and 150 rpm. The obtained crude phytase was purified using a combined purification process of ammonium sulphate precipitation and gel filtration chromatography using a 50 x 1.5 cm Sephadex G-75 column. The activity of the partially purified enzyme was assessed by subjecting it to varying pH, temperature, and nutrient conditions. The optimum temperature and pH were 50 o C and 6 respectively. The enzyme remained active for over 30 min at 70 o C and completely lost activity after 10 min at 80 o C. No activity was observed at 100 o C. The phytase produced with glucose as carbon source had higher activity (0.087 U/mL) than that produced with sucrose (0.018 U/mL). The A. niger isolate used in this study was unable to utilize ammonium sulphate as a source of nitrogen (no activity). This study establishes the potential of indigenous fungi for industrial phytase production.

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Section: Resultsmentioning

confidence: 73%

Section: Introductionmentioning

confidence: 99%

Purification and Characterization of Phytase Produced by Aspergillus niger Isolated from Poultry Farm Soil Using Submerged Fermentation

2021

TJNPR

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“…Many Enterobacter spp. form biofilm [4,32,33]. Sabir et al [34] investigated the biofilm formation of pathogens causing urinary tract infection and their resistance to antibiotics.…”

Section: Discussionmentioning

confidence: 99%

The determination of antimicrobial and antibiofilm activities of foodborne lactic acid bacteria against Enterobacter cloacae isolates

Kenar¹,

ERİK²,

Erdoğmuş³

et al. 2020

Turk J Vet Anim Sci

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The aim of this study was to investigate the antimicrobial and antibiofilm activities of 10 different lactic acid bacteria (LAB) strains isolated from local food sources of animal origin against 4 Enterobacter cloacae isolates obtained from clinical cases and determine their adhesion potentials to intestinal epithelial cells. In this study, all Enterobacter cloacae isolates (P3, P4, P5, P7) identified with the BD Phoenix automation system were detected to form biofilm with both Congo red agar and Microtiter plate methods. Amoxicillinclavulanate, cefuroxime, and ampicillin resistance was determined in all isolates. It was determined that LAB strains producing exopolysaccharide (EPS) were able to colonize intestinal epithelial cells. It is noteworthy that LAB extracts were effective to inhibit the biofilm formation of P3Ec, which had higher antibiotic resistance than those of other isolates. Antimicrobial effect of LAB extracts on Enterobacter cloacae were also detected by both agar disc diffusion and well diffusion tests. In this study, all of the isolated LAB strains (especially L. lactis, L. fermentum, and L. casei) are good candidates for controlling Enterobacter cloacae biofilm formation. These findings indicate that L. lactis, L. fermentum, and L. casei can potentially be developed as novel antibiofilm agents.

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“…En. aerogenes is a halotolerant hydrogen-producing bacterium [24] and was characterized with its ability to produce phytase, a phosphatase enzyme catalyzing hydrolysis of organic phosphorus into inorganic phosphorus to make phosphorus readily available for plants [25]. Frequent presence of En.…”

Section: Characteristics Of Bacterial Communitymentioning

confidence: 99%

Isolation and Identification of Alkali-tolerant Bacteria from Near-Shore Soils in Dokdo Island

Namirimu¹,

Kim²,

Zo³

2019

Microbiology and Biotechnology Letters

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Screening, nutritional optimization and purification for phytase produced by Enterobacter aerogenes and its role in enhancement of hydrocarbons degradation and biofilm inhibition

Cited by 18 publications

References 21 publications

Purification and Characterization of Phytase Produced by Aspergillus niger Isolated from Poultry Farm Soil Using Submerged Fermentation

Purification and Characterization of Phytase Produced by Aspergillus niger Isolated from Poultry Farm Soil Using Submerged Fermentation

The determination of antimicrobial and antibiofilm activities of foodborne lactic acid bacteria against Enterobacter cloacae isolates

Isolation and Identification of Alkali-tolerant Bacteria from Near-Shore Soils in Dokdo Island

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