Screening method for the analysis of antiviral drugs in poultry tissues using zwitterionic hydrophilic interaction liquid chromatography/tandem mass spectrometry

Chan, Danny; Tarbin, J. A.; Sharman, Matthew; Carson, Mary C; Smith, Michelle; Smith, S.

doi:10.1016/j.aca.2010.11.015

Cited by 39 publications

(36 citation statements)

References 5 publications

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“…1,6,9 The results showed that the recovery of ACV by MIP (98.2%) was obviously higher than NIP (76.8%) and the other sorbents (#67%), indicating the high affinity of the MIP to ACV (Fig. 5).…”

Section: Comparison With Different Dispersantsmentioning

confidence: 87%

Rapid determination of acyclovir in edible creatural tissues by molecularly imprinted matrix solid-phase dispersion coupled with high performance liquid chromatography

et al. 2013

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A simple, rapid and selective sample pretreatment method, molecularly imprinted matrix solid-phase dispersion (MI-MSPD) coupled with liquid chromatography, was developed for the rapid isolation of acyclovir (ACV) from edible creatural tissues. New molecularly imprinted polymer (MIP) synthesized by using theophylline as a dummy template revealed a high special affinity to ACV, and was employed as the selective dispersant of matrix solid-phase dispersion (MSPD) for the rapid screening of ACV. The presented method obviously reduced the tedious separation procedure, improved the extraction selectivity and purification efficiency, and significantly eliminated the effect of template leakage of MIP on quantitative analysis. The results indicated that the interference compounds originating from the creatural tissue matrix could be efficiently eliminated, and the elution was clean enough for HPLC analysis. Good linearity of ACV was obtained in a range of 0.10-50.0 mg g À1 with r 2 ¼ 0.9998, and the limit of quantification based on the signal to noise of 10 was 0.09 mg g À1 . The recoveries at three spiked levels were in the range of 85.5-108.1% with RSD # 3.7% (n ¼ 3). The presented MI-MSPD method combined the advantages of MIP and MSPD, and could be applied for the rapid and selective screening of ACV in creatural tissues.

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Section: Comparison With Different Dispersantsmentioning

confidence: 87%

Rapid determination of acyclovir in edible creatural tissues by molecularly imprinted matrix solid-phase dispersion coupled with high performance liquid chromatography

et al. 2013

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“…This manipulation has facilitated the study of the molecular mechanisms behind the virulence, pathogenicity and evolution of NDV. To date, all NDV strains isolated worldwide have been classified into two major categories: class I and class II [10,24-26]. Class II contains the well-researched NDV strain genotypes, while class I viruses were only identified in 2006 and have been studied less intensely [10].…”

Section: Discussionmentioning

confidence: 99%

Rescue of virulent class I Newcastle disease virus variant 9a5b-D5C1

Qiu

et al. 2012

Virol J

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BackgroundThe virulent class I Newcastle disease virus (NDV) variant 9a5b was generated from a nonvirulent NDV isolate Goose/Alaska/415/91 via nine consecutive passages in the chicken air sac, followed by five passages in the chick brain. The evolutionary mechanism of virulence in the class I NDV isolate is not fully understood. To elucidate this evolutionary mechanism, a reverse genetics manipulation specific for class I NDV is indispensable.ResultsA full-length cDNA clone of 9a5b and the helper plasmids pCI-NP, pCI-P, and pCI-L were constructed from segments of cDNA. After these plasmids were co-transfected into BSR T7/5 cells, infectious viral particles were obtained. The rescued viruses were genetically and biologically identical to the parental strain and showed similar pathogenicity in chickens.ConclusionA stable recovery method for class I NDV was established. Reverse genetics of the class I NDV variant 9a5b allowed for the generation of genetically altered and virulent NDV, and can be used as a foundation for research on the evolution of virulence in class I NDV isolates.

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“…The total chromatographic run time is 5 min, including analysis, column cleaning and column equilibration, which is amenable to the high-throughput requirements of clinical study analyses. Previous literature methods that report comparable analysis run times (3 to 8 minutes) cited 10-to 250-fold higher LLOQs (10 ng/mL to 200 ng/mL) [25][26][27][28]. Previous LC-MS/MS methodology that reports a comparable LLOQ (2 ng/mL) had a 4-fold longer analysis run time (20 min) [24].…”

Section: Chromatographymentioning

confidence: 92%

“…This method is the most sensitive, to our knowledge, with a LLOQ that is between 2-fold and 250-fold better than other assays [14 -28]. Typical LLOQs varied for previous acyclovir assays with the most sensitivity reported for MS/MS detection: MS/ MS detection, 2 ng/mL to 200 ng/mL [24][25][26][27][28]; UV detection, 10 ng/ mL to 250 ng/mL [14-18]; and fluorescence detection, 3.25 ng/mL to 30 ng/mL [19][20][21][22]. This methods's 1 ng/mL LLOQ is important to be able to detect and accurately quantify acyclovir concentrations at all of the study timepoints at the given dose and study conditions: patient plasma at the longest time points frequently contained < 10 ng/mL acyclovir.…”

Section: Sensitivitymentioning

confidence: 96%

“…Previous analytical methods for acyclovir include immunological techniques [13], high-performance liquid chromatography (HPLC) with ultraviolet (UV) detection [14][15][16][17][18], HPLC with fluorescence detection [19][20][21][22], and LC-MS/MS [23][24][25][26][27][28]. Unfortunately, many UV-, fluorescence-, and LC-MS/MS-based assays lack sensitivity with lower limit of quantification (LLOQ) between 10 ng/mL and 250 ng/mL [14][15][16][17][18][19][20][21]23,[25][26][27][28]. Recently, MS/MS Page 2 of 6 detection has improved sensitivity 5-fold, lowering reported LLOQs to 2 ng/mL, but the analysis time of 20 min for this method is undesirable for clinical studies with thousands of samples [24].…”

Section: Introductionmentioning

confidence: 99%

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Quantification of Acyclovir in Human Plasma by Ultra-High-Performance Liquid Chromatography - Heated Electrospray Ionization - Tandem Mass Spectrometry for Bioequivalence Evaluation

Shao¹

2012

J Anal Bioanal Techniques

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Pharmacokinetic studies are essential towards determining bioequivalence and establishing pharmacokinetic profiles for drug moieties requires accurate quantification. We report a rapid, sensitive, and robust method for the determination of acyclovir in human plasma and its validation towards evaluating the bioequivalence of drug formulations. After a simple liquid-liquid extraction from plasma, acyclovir is quantified using ultra-high-performance liquid chromatography -heated electrospray ionization -tandem mass spectrometry (UHPLC-HESI-MS/MS). The assay has a total analysis time is 5 min, a linear range of 1.0 -2000 ng/mL, a lower limit of detection of 0.5 ng/ mL, and a lower limit of quantification of 1.0 ng/mL. Intra-and inter-day precision is no more than 10.3% and intraand inter-day accuracy was within 13% at various concentrations in human plasma. Validation according to FDA guidelines for bioanalysis indicates that the described UHPLC-HESI-MS/MS method provides rigorous quantification of acyclovir in human plasma and representative data demonstrates successful application towards the determination of pharmacokinetic profiles as part of an evaluation of drug formulation bioequivalence.

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Screening method for the analysis of antiviral drugs in poultry tissues using zwitterionic hydrophilic interaction liquid chromatography/tandem mass spectrometry

Cited by 39 publications

References 5 publications

Rapid determination of acyclovir in edible creatural tissues by molecularly imprinted matrix solid-phase dispersion coupled with high performance liquid chromatography

Rapid determination of acyclovir in edible creatural tissues by molecularly imprinted matrix solid-phase dispersion coupled with high performance liquid chromatography

Rescue of virulent class I Newcastle disease virus variant 9a5b-D5C1

Quantification of Acyclovir in Human Plasma by Ultra-High-Performance Liquid Chromatography - Heated Electrospray Ionization - Tandem Mass Spectrometry for Bioequivalence Evaluation

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