Screening fungal endophytes from a wild grass for growth promotion in tritordeum, an agricultural cereal

Aldana, Beatriz R. Vázquez de; Arellano, Juan B.; Cuesta, Marcelino; Mellado-Ortega, Elena; González, Virginia; Zabalgogeazcoa, Íñigo

doi:10.1016/j.plantsci.2020.110762

Cited by 15 publications

(17 citation statements)

References 41 publications

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“…Additionally, fungal endophytes can induce the formation non–enzymatic antioxidant metabolites such as phenolic compounds ( White and Torres, 2010 ; Bacon and White, 2016 ; Varela et al., 2016 ). In our previous studies, Diaporthe EB4 did not enhance the total phenolic content in grasses under control or salt stress conditions ( Vázquez de Aldana et al., 2021 ; Toghueo et al., 2022 ). In the present study, we obtained rather similar results and Diaporthe seemed to induce a decline in the non–enzymatic antioxidant capacity under drought stress and to have no significant effect on the total phenolic content.…”

Section: Discussionmentioning

confidence: 77%

“…Additionally, fungal endophytes can induce the formation nonenzymatic antioxidant metabolites such as phenolic compounds (White and Torres, 2010;Bacon and White, 2016;Varela et al, 2016). In our previous studies, Diaporthe EB4 did not enhance the total phenolic content in grasses under control or salt stress conditions (Vaźquez de Aldana et al, 2021;Toghueo et al, 2022).…”

Section: Discussionmentioning

confidence: 82%

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Diaporthe atlantica enhances tomato drought tolerance by improving photosynthesis, nutrient uptake and enzymatic antioxidant response

et al. 2023

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Functional symbiosis with fungal endophytes can help plants adapt to environmental stress. Diaporthe atlantica is one of the most abundant fungal taxa associated with roots of Festuca rubra subsp. pruinosa, a grass growing in sea cliffs. This study aimed to investigate the ability of a strain of this fungus to ameliorate the impact of drought stress on tomato plants. In a greenhouse experiment, tomato plants were inoculated with Diaporthe atlantica strain EB4 and exposed to two alternative water regimes: well-watered and drought stress. Several physiological and biochemical plant parameters were evaluated. Inoculation with Diaporthe promoted plant growth in both water treatments. A significant interactive effect of Diaporthe-inoculation and water-regime showed that symbiotic plants had higher photosynthetic capacity, water-use efficiency, nutrient uptake (N, P, K, Fe and Zn), and proline content under drought stress, but not under well-watered conditions. In addition, Diaporthe improved the enzymatic antioxidant response of plants under drought, through an induced mechanism, in which catalase activity was modulated and conferred protection against reactive oxygen species generation during stress. The results support that Diaporthe atlantica plays a positive role in the modulation of tomato plant responses to drought stress by combining various processes such as improving photosynthetic capacity, nutrient uptake, enzymatic antioxidant response and osmo-protectant accumulation. Thus, drought stress in tomato can be enhanced with symbiotic fungi.

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Section: Discussionmentioning

confidence: 77%

Section: Discussionmentioning

confidence: 82%

Diaporthe atlantica enhances tomato drought tolerance by improving photosynthesis, nutrient uptake and enzymatic antioxidant response

et al. 2023

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“…More broadly, Sordariomycetes, and genera within that lineage, such as Coniochaeta , are commonly isolated from various hosts in temperate and boreal zones (U'Ren et al ., 2010, 2012; Rosa et al ., 2013). Some strains of Coniochaeta have been observed to promote plant growth in other systems, such as in grass (Challacombe et al ., 2019; Vázquez de Aldana et al ., 2021). Our results provide evidence that the growth‐promoting ability of Coniochaeta could be widespread among plants, including mosses.…”

Section: Discussionmentioning

confidence: 99%

Comparative transcriptomics of fungal endophytes in co‐culture with their moss host Dicranum scoparium reveals fungal trophic lability and moss unchanged to slightly increased growth rates

et al. 2022

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Mosses harbor fungi whose interactions within their hosts remain largely unexplored. Trophic ranges of fungal endophytes from the moss Dicranum scoparium were hypothesized to encompass saprotrophism. This moss is an ideal host to study fungal trophic lability because of its natural senescence gradient, and because it can be grown axenically.Dicranum scoparium was co-cultured with each of eight endophytic fungi isolated from naturally occurring D. scoparium. Moss growth rates, and gene expression levels (RNA sequencing) of fungi and D. scoparium, were compared between axenic and co-culture treatments. Functional lability of two fungal endophytes was tested by comparing their RNA expression levels when colonizing living vs dead gametophytes.Growth rates of D. scoparium were unchanged, or increased, when in co-culture. One fungal isolate (Hyaloscyphaceae sp.) that promoted moss growth was associated with differential expression of auxin-related genes. When grown with living vs dead gametophytes, Coniochaeta sp. switched from having upregulated carbohydrate transporter activity to upregulated oxidation-based degradation, suggesting an endophytism to saprotrophism transition. However, no such transition was detected for Hyaloscyphaceae sp.Individually, fungal endophytes did not negatively impact growth rates of D. scoparium. Our results support the long-standing hypothesis that some fungal endophytes can switch to saprotrophism.

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“…Because of their economic impact, the best-known species are pathogenic, but reports about Diaporthe endophytes are increasing. Studies on the effects of endophytic Diaporthe species are scarce, but some reports show their involvement in plant growth promotion ( Yang et al, 2014 ; Chithra et al, 2017 ; Vazquez de Aldana et al, 2021 ; da Silva Santos et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

A Diaporthe Fungal Endophyte From a Wild Grass Improves Growth and Salinity Tolerance of Tritordeum and Perennial Ryegrass

et al. 2022

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Some microbiome components can provide functions that extend the capabilities of plants, increasing the environmental adaptability and performance of holobionts. Festuca rubra subsp. pruinosa is a perennial grass adapted to rocky sea cliffs, where soil and nutrients are very limited, and exposure to salinity is continuous. This study aimed to investigate if a Diaporthe fungal endophyte belonging to the core microbiome of Festuca rubra roots could improve the performance of two agricultural grasses. In a greenhouse experiment, plants of tritordeum (Triticum durum x Hordeum chilense) and perennial ryegrass (Lolium perenne) were inoculated with Diaporthe strain EB4 and subjected to two salinity conditions (0 and 200 mM NaCl). Biomass production, mineral elements, proline, hormone profiles, antioxidant capacity, and total phenolic compounds were examined in plants, and fungal functions potentially related to the promotion of plant growth were determined. The inoculation with Diaporthe promoted plant growth of both grasses, increasing leaf biomass (84% in tritordeum and 29% in perennial ryegrass), root biomass, nutrient content (N, Ca, Mg, and Fe), and the production of indole 3-acetic acid, regardless of the salinity treatment. Improved growth and nutrient uptake might occur because Diaporthe produces several extracellular enzymes capable of recycling organic nutrient pools. In addition, the fungus produced indole 3-acetic acid in vitro and modulated the production of this phytohormone in the plant. Under salinity, the activity of Diaporthe ameliorated the stress, increasing proline, nutrient uptake in roots, gibberellins, and indole 3-acetic acid, which in turn results into improved growth. Thus, this fungus can transfer to alternative hosts some advantages useful at its original habitat.

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Screening fungal endophytes from a wild grass for growth promotion in tritordeum, an agricultural cereal

Cited by 15 publications

References 41 publications

Diaporthe atlantica enhances tomato drought tolerance by improving photosynthesis, nutrient uptake and enzymatic antioxidant response

Diaporthe atlantica enhances tomato drought tolerance by improving photosynthesis, nutrient uptake and enzymatic antioxidant response

Comparative transcriptomics of fungal endophytes in co‐culture with their moss host Dicranum scoparium reveals fungal trophic lability and moss unchanged to slightly increased growth rates

A Diaporthe Fungal Endophyte From a Wild Grass Improves Growth and Salinity Tolerance of Tritordeum and Perennial Ryegrass

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