Screening for the Immunopotentiating Activity of Food Microorganisms and Enhancement of the Immune Response by<i>Bifidobacterium adolescentis</i>M101-4

Lee, Jong-Hwa; Ametani, Akio; Enomoto, Atsushi; Sato, Yuichi; Motoshima, Hidemasa; Ike, Fumio; Kaminogawa, Shuichi

doi:10.1271/bbb.57.2127

Cited by 50 publications

(29 citation statements)

References 6 publications

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“…However, the interactions between probiotic bacteria and mucosal immune cells in the gut are not well understood. Lee et al reported that bifidobacteria have potent immunopotentiating activity among food microorganisms, and that Bifidobacterium adolescentis M101-4 (B. pseudocatenulatum 7041), derived from human intestinal microflora, had the strongest mitogenic activity on splenocytes and Peyer's patch cells (Lee et al 1993). In addition, they found that this activity increased after disruption.…”

Section: Introductionmentioning

confidence: 99%

Characteristic Immune Response in Peyer’s Patch Cells Induced by Oral Administration of Bifidobacterium Components

et al. 2005

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We demonstrate immunomodulatory effects, especially those involving murine intestinal IgA secretion, in Peyer's patch cells following oral administration of Bifidobacterium immunomodulator (BIM) derived from sonicated B. pseudocatenulatum 7041. BALB/c mice were administered BIM orally for 7 consecutive days. The PP cells demonstrated upregulated secretion of total IgA including BIM-specific IgA following BIM administration. In observing the response of PP cells co-cultured with BIM, we found enhanced secretion of interferon-gamma (IFN-gamma) and interleukin (IL)-6 in the CD4(+) T cells. In contrast, IL-12 secretion by Thy1.2(-) PP cells was enhanced, but secretion of IFN-gamma, IL-5, and IL-6 was not significantly affected. Furthermore, the population of CD4(+) CD45RB(high) T cells in PP increased following oral administration of BIM. These data suggest that CD4(+) T cells were affected by BIM administration. Overall, the results show that oral administration of BIM induced CD4(+) PP cells to change their expression of cell surface antigen and cytokine production.

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Section: Introductionmentioning

confidence: 99%

Characteristic Immune Response in Peyer’s Patch Cells Induced by Oral Administration of Bifidobacterium Components

et al. 2005

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“…Recently, several kinds of dairy and pharmaceutical products containing living bifidobacteria have been proposed as dietary adjuncts. Indications are that therapeutic use of orally administered bifidobacteria offers probiotic effects such as anti-tumorigenic activity (Reddy and Riverson 1993), immunological enhancement (Lee et al 1993), maintenance of a proper balance of the normal intestinal microflora (Ibrahim and Bezkorovainy 1993), reduction of serum cholesterol levels, improvement of lactose intolerance and synthesis of B-complex vitamins (Tannok 1999).…”

Section: Introductionmentioning

confidence: 99%

Nucleotide sequence, expression and transcriptional analysis of the Bifidobacterium longum MB 219 lacZ gene

Rossi

Altomare

et al. 2000

Archives of Microbiology

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The gene encoding beta-galactosidase was isolated by functional complementation of Escherichia coli from Bifidobacterium longum MB219, which exhibited the highest activity among ten Bifidobacterium strains tested of the species B. longum, B. breve, B. adolescentis, B. indicum, B. animalis and B. cuniculi. The nucleotide sequence of the 5.0-kb fragment conferring the positive beta-galactosidase phenotype to E. coli revealed the presence of a lacZ-type gene encoding a 1023-amino-acid protein that was preceded by a ribosome binding site. A sequence showing 72% identity with the proline tRNA of Bacillus subtilis and a gene probably encoding the DNA-3-methyladenine glycosydase I were located downstream from the lacZ gene, after a gap of 30-50 unsequenced base pairs. By primer-extension analysis, the transcription start site of the lacZ gene was mapped 65 nt upstream from the start codon, and it enabled identification of the -10 region of the putative promoter. The nucleotide sequence of lacZ and its deduced amino acid sequence were compared with those of beta-galactosidase genes and enzymes from other microorganisms. High similarity was demonstrated between the B. longum beta-galactosidase and its counterparts in Lactobacillus delbruckii subsp. bulgaricus, Streptococcus salivarius subsp. thermophilus, E. coli, Clostridium acetobutylicum, Leuconostoc lactis, Klebsiella pneumoniae and Kluyveromyces marxianus var. lactis, all belonging to the LacZ family. The B. longum MB219 lacZ gene was cloned in Bifidobacterium and its expression was observed in strains with otherwise low levels of endogenous activity. The expression increased by factors of 1.5-50 and enabled those strains that do not grow on lactose to use this sugar as sole carbon source.

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“…1). Lee et al (1993) reported that Bifidobacterium adolescentis M101-4, which was the origin of Bp and derived from human intestinal microflora, had the strongest mitogenic activity on the lymphocytes of mice. In addition, this activity was increased by destruction of the organism.…”

Section: Discussionmentioning

confidence: 99%

“…Bifidobacterium pseudocatenulatum JCM 7041 (Bp), isolated through screening by Lee et al (1993) was obtained from RIKEN (Bioresource, Japan collection of microorganisms, Tokyo, Japan). Bp was cultured in GAM broth at 37°C for 18 h.…”

Section: Bacteriamentioning

confidence: 99%

Bifidobacterium components have immunomodulatory characteristics dependent on the method of preparation

et al. 2007

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Some bifidobacteria or lactobacilli exhibit a variety of immunomodulatory effects, such as being antiinflammatory, increasing IgA secretion, and moderating allergy. We prepared three types of Bifidobacterium components from B. pseudocatenulatum JCM 7041 (Bp) using preparation methods such as sonication, heat treatment, and non-treatment (live Bp). Furthermore, we compared their immunomodulatory effects using in vivo and in vitro immunological bio-assays. We determined immune responses such as cell proliferation and the production of cytokines and IgA in Peyer's patch cells in vitro following co-culture with bacterial components, and investigated the effects of oral administration of each of them on cytokine and IgA production by Peyer's patch cells. Live-, ultrasonic treated-and heat-treated Bp exhibited cytokineinducing and cell proliferation activities. Sonicated Bp in particular showed the greatest immunomodulatory activity in the short term as measured by in vitro and in vivo assays, while heat-treated Bp induced cytokines (e.g. IL-6 and IFN-c) and IgA production following oral administration for 7 consecutive days. These data showed that Bifidobacterium components prepared by different methods might induce different immune responses. Using scanning electron microscopy we demonstrated that the surface structure of sonicated Bp, which contained more soluble saccharides, was different from other components. These data suggest that the immunomodulatory effect of Bp is dependent upon the bacterial conformation and condition.

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Screening for the Immunopotentiating Activity of Food Microorganisms and Enhancement of the Immune Response byBifidobacterium adolescentisM101-4

Cited by 50 publications

References 6 publications

Characteristic Immune Response in Peyer’s Patch Cells Induced by Oral Administration of Bifidobacterium Components

Characteristic Immune Response in Peyer’s Patch Cells Induced by Oral Administration of Bifidobacterium Components

Nucleotide sequence, expression and transcriptional analysis of the Bifidobacterium longum MB 219 lacZ gene

Bifidobacterium components have immunomodulatory characteristics dependent on the method of preparation

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