Characteristic Immune Response in Peyer’s Patch Cells Induced by Oral Administration of Bifidobacterium Components

Abstract

We demonstrate immunomodulatory effects, especially those involving murine intestinal IgA secretion, in Peyer's patch cells following oral administration of Bifidobacterium immunomodulator (BIM) derived from sonicated B. pseudocatenulatum 7041. BALB/c mice were administered BIM orally for 7 consecutive days. The PP cells demonstrated upregulated secretion of total IgA including BIM-specific IgA following BIM administration. In observing the response of PP cells co-cultured with BIM, we found enhanced secretion of interferon-gamma (IFN-gamma) and interleukin (IL)-6 in the CD4(+) T cells. In contrast, IL-12 secretion by Thy1.2(-) PP cells was enhanced, but secretion of IFN-gamma, IL-5, and IL-6 was not significantly affected. Furthermore, the population of CD4(+) CD45RB(high) T cells in PP increased following oral administration of BIM. These data suggest that CD4(+) T cells were affected by BIM administration. Overall, the results show that oral administration of BIM induced CD4(+) PP cells to change their expression of cell surface antigen and cytokine production.

Bifidobacterium components have immunomodulatory characteristics dependent on the method of preparation

“…We previously reported that oral administration of BIM induced IgA production by PP cells and cytokine production related to IgA production (e.g. IL-6 and IFN-c) by CD4 + cells derived from PP (Nakanishi et al 2005). It suggested that PP CD4 T cell might be activated to induce IgA production by oral administration of Bp components.…”

“…Peyer's patch (PP) cells (2.5 9 10 5 cells mL -1 ) were obtained from 7-week-old female BALB/c mice, and cultured with each prepared Bp component (1 9 10 7 CFU) in RPMI 1640 medium containing 5% FCS in 96-well plates for 36 h. The proliferation activity of PP cells was measured by the [ 3 H]-thymidine uptake test according to a previously reported method (Hosono et al 1997 ) for 24 h. After co-cultivation, PP culture supernatant was obtained and IL-12p40 was measured by ELISA according to the previously reported methods (Hosono et al 1998;Nakanishi et al 2005).…”

“…Mice in the control group were administered the same amount of PBS only. After oral administration for single-shot or 7 consecutive days, cells were prepared from PP, and the amount of IgA, IL-6, IL12p40 and IFN-c produced by PP cells was measured by ELISA according to the previously reported methods (Hosono et al 1998;Nakanishi et al 2005).…”

“…Lactobacillus paracasei (NCC 2461) was associated with increased IgG2a in the serum (IbnouZekri et al 2003). We previously reported that when Bifidobacterium immunomodulator (BIM), prepared by ultrasonic disintegration, and lyophilized Bifidobacterium pseudocatenulatum JCM 7041 (Bp) was administered orally to female BALB/c mice for 7 consecutive days, IgA production and some cytokine production in Peyer's patches of the small intestine increased (Nakanishi et al 2005). Taken together, these studies indicated that the variety of immunomodulatory effects by LAB was dependent on the bacterial species.…”

Immunomodulatory Effects of <i>Lactobacillus</i> and <i>Bifidobacterium </i>on Both Murine and Human Mitogen-Activated T Cells

“…On the other hand, Bifidobacterium is a dominant genus of infants’ fecal flora that represent up to 90% of the total gut microflora in breast-fed babies [19] and up to 15% of that in adults [20]. Some strains of the genus Bifidobacterium exhibit anti-inflammatory properties [21,22,23], increase intestinal IgA secretion [24], and induce dendritic cell maturation [25]. …”