Screening for Salt Tolerance in Four Local Varieties of Phaseolus lunatus from Spain

Arteaga, Sugenith; Hassan, Mohamad Al; Bandara, Wijesinghe M. Chaminda; Yabor, Lourdes; Lull, Cristina; Boscaiu, Mónica; Vicente, Óscar

doi:10.3390/agriculture8120201

Cited by 13 publications

(13 citation statements)

References 42 publications

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“…Both 'cultivar' and 'treatment', as well as their interaction, had a highly-significant effect on (practically) all growth traits analysed, and on Pro contents, for the twoand three-week treatments of both water deficit and salt stress. In all cases, growth inhibition was mostly reflected in the stress-induced reduction of fresh weight and water contents of stems (SFW and SWC) and leaves (LFW and LWC), as reported in the same species [39,40] or other species of this genus [43]. These parameters are the growth variables most significantly correlated, positively, with the first principal component in the PCA.…”

Section: Discussionsupporting

confidence: 66%

“…However, Pro can be toxic for certain tissues if it is partially catabolised to pyrroline-5-carboxylate (P5C), leading to apoptosis [53]. Considering the multiple functions of Pro, it is logical to assume that Pro accumulation would be associated with higher stress tolerance, and this has indeed been demonstrated for many plants, both wild species [54,55] and crops [43,56]. However, other comparative studies on related taxa, such as species of the same genus or cultivars or varieties of the same species, revealed higher Pro accumulation under stress in the less-tolerant genotypes [57,58].…”

Section: Discussionmentioning

confidence: 99%

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The Use of Proline in Screening for Tolerance to Drought and Salinity in Common Bean (Phaseolus vulgaris L.) Genotypes

et al. 2020

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The selection of stress-resistant cultivars, to be used in breeding programmes aimed at enhancing the drought and salt tolerance of our major crops, is an urgent need for agriculture in a climate change scenario. In the present study, the responses to water deficit and salt stress treatments, regarding growth inhibition and leaf proline (Pro) contents, were analysed in 47 Phaseolus vulgaris genotypes of different origins. A two-way analysis of variance (ANOVA), Pearson moment correlations and principal component analyses (PCAs) were performed on all measured traits, to assess the general responses to stress of the investigated genotypes. For most analysed growth variables and Pro, the effects of cultivar, treatment and their interactions were highly significant (p < 0.001); the root morphological traits, stem diameter and the number of leaves were mostly due to uncontrolled variation, whereas the variation of fresh weight and water content of stems and leaves was clearly induced by stress. Under our experimental conditions, the average effects of salt stress on plant growth were relatively weaker than those of water deficit. In both cases, however, growth inhibition was mostly reflected in the stress-induced reduction of fresh weight and water contents of stems and leaves. Pro, on the other hand, was the only variable showing a negative correlation with all growth parameters, but particularly with those of stems and leaves mentioned above, as indicated by the Pearson correlation coefficients and the loading plots of the PCAs. Therefore, in common beans, higher stress-induced accumulation of Pro is unequivocally associated with a stronger inhibition of growth; that is, with a higher sensitivity to stress of the corresponding cultivar. We propose the use of Pro as a suitable biochemical marker for simple, rapid, large-scale screenings of bean genotypes, to exclude the most sensitive, those accumulating higher Pro concentrations in response to water or salt stress treatments.

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Section: Discussionsupporting

confidence: 66%

Section: Discussionmentioning

confidence: 99%

The Use of Proline in Screening for Tolerance to Drought and Salinity in Common Bean (Phaseolus vulgaris L.) Genotypes

et al. 2020

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“…Agriculture 2019, 9, x FOR PEER REVIEW 3 of 18 [19][20][21], among many others. Similar comparative studies were performed by our group on different species of Phaseolus [22][23][24]. In this work, we present an exhaustive morphological characterization of 24 accessions of common bean local landraces stored at the seed bank of COMAV; they were chosen, maximizing their geographical origin and their morphological diversity, for studying their adaptation to drought and salinity (manuscript in preparation).…”

Section: Seed Materialsmentioning

confidence: 84%

Morphological and Agronomic Characterization of Spanish Landraces of Phaseolus vulgaris L.

et al. 2019

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Beans (Phaseolus vulgaris L.) originated on the American continent, specifically in the Mesoamerican zone, and their domestication took place independently in the Mesoamerican area and the Andean zone, giving rise to two well-differentiated genetic pools. It was also noted that the Andean wild populations originated from only a few thousand individuals from the Mesoamerican wild populations, which produced a great bottleneck in the formation of the Andean population. During centuries of cultivation in the Iberian Peninsula after its introduction in the 16th century, beans adapted to new environments, evolving numerous local landraces. Twenty-four local landraces of P. vulgaris from Spain were analyzed in the greenhouse during two consecutive seasons. From each genotype, five plants were grown and characterized for 17 quantitative and 15 qualitative traits using the International Board for Plant Genetic Resources (IBPGR) descriptors. Data were analyzed statistically by analysis of variance (ANOVA), principal component analysis (PCA), and cluster analysis. The results obtained indicate a high variability for most traits, especially those related to the yield and its components. The PCA and cluster analysis separated the landraces according to the color of the seed, the yield, and the pod and seed traits related to yield. Numerous traits exhibited interactions between the genotype and the environment. Most accessions reached higher yields in spring, in which solar radiation favors photosynthesis and, consequently, photoassimilation. The different response to the changing environment of the set of accessions studied in the present work is of great interest, and it can be exploited in breeding cultivars adapted to a broader range of environmental conditions.

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“…Faced with a situation of stress, plants divert resources (energy and metabolic precursors) from normal metabolism and biomass accumulation, to the activation of defense mechanisms, which causes the arrest or drastic reduction of growth [42,43]. For this reason, differences in stem length, fresh weight, or dry weight of plants submitted to stress treatments-in comparison to those from control-are optimal for ranking genotypes according to their degree of stress tolerance [44,45], but they are not always obvious when dealing with slow-growth woody species. However, European larch has a fast growing rate compared to other conifers [46] and effects of one month of drought stress could be noticed in the reduction of stem length growth, as also reported under salt stress [47].…”

Section: Discussionmentioning

confidence: 99%

Responses to Drought in Seedlings of European Larch (Larix decidua Mill.) from Several Carpathian Provenances

Plesa

Hassan

González-Orenga

et al. 2019

Forests

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European larch (Larix decidua Mill.) has been reported either as more tolerant or as more sensitive to drought than conifers with perennial leaves. Previous studies have revealed that Carpathian populations of European larch display a high genetic variability. A comparative study of the responses of these populations to drought stress at the seedling stage might allow the identification of drought tolerant genotypes and reliable drought stress biomarkers, which could be eventually used for the early detection of drought effects in larch, not only under control greenhouse conditions, but also in their natural stands. Growth responses were analyzed in larch seedlings from six Romanian Carpathian populations, submitted to one month of mild drought stress under controlled conditions. Levels of photosynthetic pigments (chlorophylls a and b, and carotenoids), osmolytes (proline and total soluble sugars), monovalent cations (Na+ and K+), and malondialdehyde (MDA) and non-enzymatic antioxidants (total phenolics and flavonoids) were compared with control treatments and between populations. Growth and the pattern of the biochemical responses were very similar in the six populations. Drought stress lead to stem length decrease in all population, whereas reduction of fresh weight of needles was significant only in one population (BVVC), and reduction of water content of needles in two populations (BVVC and GuHo). The optimal biochemical traits for an early detection of drought symptoms in this species is the increase—in most populations—of total soluble sugars, MDA, and total phenolic compounds, whereas K+ reduction was significant in all populations. Photosynthetic pigments remained unchanged, except for the Anin population where they were reduced under stress. Multivariate principal component and hierarchical clustering analyses confirmed the impact of drought in the growth and physiology of European larch, and revealed that the humidity of the substrate was positively correlated with the growth parameters and the levels of K+ in needles, and negatively correlated with the levels of MDA, total soluble sugars, total phenolic compounds, and flavonoids in needles.

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Screening for Salt Tolerance in Four Local Varieties of Phaseolus lunatus from Spain

Cited by 13 publications

References 42 publications

The Use of Proline in Screening for Tolerance to Drought and Salinity in Common Bean (Phaseolus vulgaris L.) Genotypes

The Use of Proline in Screening for Tolerance to Drought and Salinity in Common Bean (Phaseolus vulgaris L.) Genotypes

Morphological and Agronomic Characterization of Spanish Landraces of Phaseolus vulgaris L.

Responses to Drought in Seedlings of European Larch (Larix decidua Mill.) from Several Carpathian Provenances

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