Screening for probiotic of lactic acid bacteria isolated from the digestive tract of a native Aceh duck (Anas platyrhynchos)

Abstract

Abstract. Risna YK, Harimurti S, Wihandoyo, Widodo. 2020. Screening for Probiotic of lactic acid bacteria isolated from the digestive tract of a native Aceh duck (Anas platyrhynchos). Biodiversitas 21: 3001-3007. The lactic acid bacteria (LAB) isolated from the digestive tract of a native Aceh duck has emerged as a potential probiotic supplement for duck feed. In this study, we isolated the LAB from the digestive tract of an Aceh duck (Anas platyrhynchos) and identified the species and bacterial characteristics. Additionally, we isolated the LAB used in this study from the crop, proventriculus, ventriculus, duodenum, jejunum, ileum, and cecum of the native Aceh duck. Identification included the examination of morphology and physiology, followed by molecular identification by using 16S rRNA and sequence similarity tests for the bile salt hydrolase (bsh) gene. Bacterial isolation from the digestive tract of this duck resulted in 19 isolates with gram-positive, negative catalase, and non-motile characteristics. Of the 19 isolates, 11 isolates exhibited the ability to grow at 15°C, 37°C, and 45°C, with optimum growth at 37°C. From 11 isolates, only 6 were identified by using 16S rRNA primers and 5 were identified by using BSH primers. The identified LAB included Lactobacillus plantarum, Lactobacillus fermentum, and Pediococcus acidilactici. Hence, we conclude that Lactobacillus sp and Pediococcus sp. are potential probiotics that can be isolated and administered for duck feed.

The Effect of Different Temperatures on Addition of Powdered Lactobacillus plantarum Dad-13 on Peanut Chocolate Jam Towards Total Lactic Acid Bacteria (LAB), Sublethal Injury, pH, and Total Acids

“…According to Risna et al (2022) lag phase is where the bacteria still adjust to the new environment, so the growth is slow and the metabolic process has not been maximized. In addition, the process of making peanut chocolate jam did not involve the incubation process so that the bacteria did not have enough time to produce a metabolic product optimally.…”

Enzyme characterization of lactic acid bacteria isolated from duck excreta

“…The previous studies have reported that LAB can be obtained from the GIT of duck. LABs were isolated from the small intestine [20,21], cecum, and colon of Muscovy ducks [22] or from the crop until the cecum of Aceh ducks [23]. Ducks can survive in various agroclimatic conditions [24].…”

Egg quality and production by the addition of dried consortium probiotics on late-phase laying hens

“…Lactic acid bacteria has the ability to secrete antibacterial substances such as lactic acid, bacteriocin, and active peptides that could inhibit the growth of pathogenic bacteria, and metabolite substances with antioxidant activities such as butyrate and folate [6,7]. Antioxidants produced by the probiotics are useful to reduce free radicals that causes oxidative stress [8].…”