Screening for Potential Antibreast Cancer Components From <i>Prunellae Spica</i> Using MCF-7 Cell Extraction Coupled with HPLC-ESI-MS/MS

Yang, Anping; Zhang, Zheng; Liu, Fang; Jing, Liu; Yang, Hua; Huang, Zai-jun; Huang, Peiying; Liu, Hui

doi:10.1177/1934578x20931965

Cited by 3 publications

(3 citation statements)

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“…Another team of Chinese researchers quantified 4 phenol-carboxylic acids using an HPLC-ESI-MS/MS method for the screening of potential antineoplastic compounds in breast cancer (Yang A et al): protocatechuic, caffeic, rosmarinic acids and protocatechualdehyde. The study did not analyse the other polyphenols (flavonoids) present [54]. Previously, Ba Y and Wang Y (2017), using an HPLC-UV method with DAD detector, quantified caffeic and chlorogenic acids in Prunella vulgaris L. in order to study their antiproliferative effect on cancer cells of the thyroid (K1) [6].…”

Section: Representative Images Of Ulcers and Associated Lesions In Thmentioning

confidence: 99%

Study of the Potential Antiulcerous Action of Hydroalcoholic Extracts From Prunella Vulgaris L. Of Romanian Origin

Groşan¹

2020

FARMACIA

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Prunella vulgaris L. (Lamiaceae family) species is widespread throughout the world and the fruit-spike is used in folk medicine in many countries. The Romanian resource of Prunellae spica is poorly studied from a phytochemical and pharmacological point of view; accordingly, the present study aimed to evaluate the anti-ulcer action of a hydroalcoholic extract of this species with standardized polyphenol content. The extracts used were phytochemically characterized by the total polyphenol content (TPC) and by the determination of the constituents (phenol-carboxylic acids, flavonoids) by HPLC-MS/MS. Quantitative analysis showed the presence of flavonoids in glycosidic form in fairly large quantities (hyperoside, rutoside, isoquercitrin); on the other hand, smaller quantities of aglycones (quercetol, kaempferol, apigenin) were identified and quantified. Moreover, phenolcarboxylic acids, such as caftaric, ferulic, p-coumaric acid, were measured. Two types of doses (high dose-50 mg/kg body weight (kgbw), respectively low dose 10 mg/ kgbw, values expressed in gallic acid equivalents) were administered preventively/curatively in rats with phenylbutazone-induced ulcer; ranitidine at 50 mg/kgbw was used as an activity control. Efficacy was quantified by measuring ulcer index, as well as by examination of gross features and histopathological changes. The hydroalcoholic extracts of Prunella vulgaris L. proved to have a dose-dependent antiulcerous action in curative treatment. As the titratable acidity is not decreased by the treatment, the effect is mediated by the prevention of oxidative inflammation of the mucosa. Histopathological results confirmed these in vivo results. Prunellae spica of Romanian origin is a plant resource rich in polyphenols that has shown protective effects on nonsteroidal anti-inflammatory drugs (NSAID)-related gastric ulcer. The observed effects are dose-dependent, probably mediated by inhibition of oxidative stress in the mucosa and promotion of regeneration processes. Rezumat Specia Prunella vulgaris L. (Lamiaceae) este răspândită în întreaga lume, iar fructul este folosit în medicina populară în multe țări. Resursa românească de Prunellae spica este puțin studiată din punct de vedere fitochimic și farmacologic, în consecință, prezentul studiu a avut ca scop evaluarea acțiunii anti-ulceroase a unui extract hidroalcoolic din această specie cu conținut standardizat de polifenoli. Extractele utilizate au fost caracterizate fitochimic prin conținutul total de polifenoli (TPC) și prin determinarea constituenților (acizi fenol-carboxilici, flavonoizi) prin HPLC-MS/MS. Analiza cantitativă a arătat prezența flavonoidelor în formă glicozidică în cantități mari (hiperozid, rutozid, izoquercitrin), pe de altă parte, au fost identificate și cuantificate cantități mai mici de agliconi (quercetol, kaempferol, apigenină). Mai mult, au fost cuantificați acizii fenol-carboxilici, ca acidul caftaric, ferulic, p-cumaric. Două tipuri de doze (doză mare-50 mg/kgc, respectiv doză mică 10 mg/kgc, valori exprimate în ech...

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Section: Representative Images Of Ulcers and Associated Lesions In Thmentioning

confidence: 99%

Study of the Potential Antiulcerous Action of Hydroalcoholic Extracts From Prunella Vulgaris L. Of Romanian Origin

Groşan¹

2020

FARMACIA

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“…In this regard, new data on chemical constituents from different parts of PV extracts using various solvents, which were not covered earlier in other reviews, have been added to Table 2 . The data obtained by the authors of [ 52 , 53 ] showed that rosmarinic acid was the main component of aqueous extracts of PV. To confirm this fact, [ 52 ] we additionally carried out the HPLC analysis using a standard sample of rosmarinic acid.…”

Section: Chemical Composition Of Pv Plant Partsmentioning

confidence: 99%

Prunella vulgaris L.: An Updated Overview of Botany, Chemical Composition, Extraction Methods, and Biological Activities

et al. 2023

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Prunella vulgaris L. (PV) is a well-known renewable drug resource full of different groups of biologically active substances with a wide range of pharmacological actions and applications in medicine. In this review, we present an updated comprehensive overview of the botany, extracting methods, chemical composition, and pharmacological activity of different parts of PV extracts. As a result of this review, it was found that chemical composition of PV depends on various factors ranging from the part of the plant to the method of extraction. We also highlight extraction methods that have not been previously used for obtaining PV extracts and may have high scientific interest. With this review, we hope to guide present and future professionals and provide possible previously unexplored areas to find new solutions associated with PV plant.

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“…Oleanolic acid from PVL, was proved to induce apoptosis of lung adenocarcinoma cells by down-regulating the expression of Bcl-2 and up-regulating the expression of Bax and Bad ( Feng et al, 2011b ). In addition, Yang et al proved through experiments that the protocatechuic aldehyde and caffeic acid derived from PVL exhibited obvious cytotoxicity to Michigan Cancer Foundation-7 (MCF-7) cell ( Yang et al, 2020 ).…”

Section: Pharmacologymentioning

confidence: 99%

Prunella vulgaris L. – A Review of its Ethnopharmacology, Phytochemistry, Quality Control and Pharmacological Effects

2022

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Prunella vulgaris L. (PVL) is dried fruit spike of Lamiacea plant Prunella vulgaris L., which is a perennial herb with medicinal and edible homology used for thousands of years. PVL is bitter, acrid, cold, and belongs to the liver and gallbladder meridians. It clears the liver and dissipate fire, improve vision, disperse swelling, and has satisfactory clinical therapeutic effects on many diseases such as photophobia, dizziness, scrofula, goiter, breast cancer. The collection of information and data related to PVL comes from literatures retrieved and collated from various online scientific databases (such as CNKI, VIP, PubMed, Web of Science, Research Gate, Science Database), ancient books of traditional chinese medicine (Encyclopedia of Traditional Chinese Medicine, Classics of Traditional Chinese Medicine, Dictionary of Traditional Chinese Medicine), and Doctoral and Master’s Dissertations. Currently, the major chemical constituents isolated and identified from PVL are triterpenoids, steroids, flavonoids, phenylpropanoids, organic acids, volatile oils and polysaccharides. Modern pharmacological studies have shown that PVL has a wide range of pharmacological activities, including anti-inflammatory, anti-tumor, antibacterial and antiviral effects, as well as immune regulation, antihypertensive, hypoglycemic, lipid-lowering, antioxidant, free radical scavenging, liver protection, sedative and hypnotic effects. This paper reviewes the botany, ethnopharmacology, traditional application, phytochemistry, analytical methods, quality control, pharmacological effects of PVL. It can be used not only as medicine, but also gradually integrated into the “medicine and food homology” and “Chinese medicine health” boom. More importantly, it has great potential for drug resources development. This paper deeply discusses the shortcomings of current PVL research, and proposes corresponding solutions, in order to find a breakthrough point for PVL research in the future. At the same time, it is necessary to further strengthen the research on its medicinal chemistry, mechanism of action and clinical application efficacy in the future, and strive to extract, purify and synthesize effective components with high efficiency and low toxicity, so as to improve the safety and rationality of clinical medication.

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Screening for Potential Antibreast Cancer Components From Prunellae Spica Using MCF-7 Cell Extraction Coupled with HPLC-ESI-MS/MS

Cited by 3 publications

References 19 publications

Study of the Potential Antiulcerous Action of Hydroalcoholic Extracts From Prunella Vulgaris L. Of Romanian Origin

Study of the Potential Antiulcerous Action of Hydroalcoholic Extracts From Prunella Vulgaris L. Of Romanian Origin

Prunella vulgaris L.: An Updated Overview of Botany, Chemical Composition, Extraction Methods, and Biological Activities

Prunella vulgaris L. – A Review of its Ethnopharmacology, Phytochemistry, Quality Control and Pharmacological Effects

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