Screening for polymorphism at the prion protein (PrP) locus (PRNP) in Awassi and Assaf populations in Israel, the Palestinian Authority and Jordan

Gootwine, E.; Abdulkhaliq, Ahed; Jawasreh, Khaleel; Zárate, Anne Valle

doi:10.1016/j.smallrumres.2008.02.008

Cited by 13 publications

(25 citation statements)

References 9 publications

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“…At codon 171, there were glutamine, histidine, arginine and lysine alleles, and the lysine allele was found only in Ki > vi > rci > k sheep. The same allele was reported in Mongolian (Gombojav et al, 2003), Awassi (Gootwine et al, 2008), Greek (Billinis et al, 2004) and Bergamasca sheep (Lü hken et al, 2008) at low frequencies. This study showed that all of the common alleles observed so far in the other sheep breeds were also found in the investigated Turkish sheep.…”

Section: Discussionsupporting

confidence: 80%

Prion protein gene (PrP) polymorphisms in healthy sheep in Turkey

Öner

Yeşilbağ

Tuncel

et al. 2011

Animal

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Scrapie, a transmissible spongiform encephalopathy (TSE) or prion disease, is a fatal, neurodegenerative disease in sheep and goats. This disease has been known in Europe for more than 250 years. Susceptibility to scrapie is associated with polymorphisms in the sheep prion protein gene (PrP) gene. In sheep, polymorphism in the PrP gene has been identified at a number of codons, and polymorphisms at codons 136, 154 and 171 have reported linkage with susceptibility to scrapie. Polymorphisms at the PrP locus were studied in 413 animals representing three native sheep breeds (Imroz, Chios and Kıvırcık) in Turkey. Genomic DNA was obtained from blood, and genotypes were screened using PCR and direct DNA sequencing. We report 17 genotypes derived from seven different alleles. The most frequent genotype in the Kıvırcık sheep is ARQ/ARQ, whereas the ARR/ARQ genotype is predominant in the Chios and Imroz breeds. In general, the ARQ haplotype was the predominant haplotype. ARQ haplotype was also predominant in the Kıvırcık and Chios sheep breeds, whereas the Imroz sheep predominantly had the ARR haplotype. The susceptibility-associated VRQ haplotype was found in 2.38%, 0.35% and 0.81% of the Imroz, Kıvırcık and Chios sheep, respectively. Moreover, seven additional polymorphisms have been detected at codons G127S, G127V, H143R, G145S, Y172D, N174Y and Q189L. Among these polymorphisms, the N174Y allele is a novel polymorphism, and the G145S allele is a novel allele for a known polymorphic locus.

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Section: Discussionsupporting

confidence: 80%

Prion protein gene (PrP) polymorphisms in healthy sheep in Turkey

Öner

Yeşilbağ

Tuncel

et al. 2011

Animal

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“…Higher frequencies of the ARQ allele have been reported in scrapie-affected sheep (Ekateriniadou et al, 2007a). Similar frequencies of the ARQ allele were reported in Italy (Granato et al, 2013, Martemucci et al, 2015, in Brazil (Ianella et al, 2012) and in Turkey (Meydan et al, 2012), whereas higher frequencies (74-86%) were observed in Awassi and Assaf sheep populations (Gootwine et al,2008).…”

Section: Discussionsupporting

confidence: 72%

Prion protein gene polymorphisms in classical scrapie-affected flocks of sheep in Central Macedonia

Kioutsioukis

Papadogiannakis

Palaska

et al. 2018

J Hellenic Vet Med Soc

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Research articlesheep, 340 were of Chios breed, 633 Chios crossbred and 483 crossbred. The examined sheep showed high genotype variability, as a total of 7 haplotypes and 23 different genotypes were found. The predominant allele and the predominant genotype were ARQ and ARQ/ARQ respectively, in all breeds studied, followed by the ARR allele and the ARR/ ARQ genotype. The TRQ allele was frequent in Chios and Chios crossbred, while the VRQ allele was rare for all the breeds. Interestingly, 3 genotypes (ARH/TRQ, ARR/ARK and ARK/VRQ) were detected for the first time in Greece and two of them (ARH/TRQ and ARK/VRQ) have, to our knowledge, never been previously reported. Furthermore, it is emphasized that our country outnumbers all European countries in classical scrapie cases of sheep every year.Therefore, there is an urgent need to reduce the incidence of classical scrapie through the implementation of selective breeding programs. This is supported by the fact that the prevalence of classical scrapie in the Greek sheep population is highly associated with the predominant genotype ARQ/ARQ. Therefore, the elimination of the ARQ/ARQ and the other susceptible genotypes (belonging to Risk Groups 3 and 5, according to the National Scrapie Plan of Great Britain) would reduce dramatically the incidence of classical scrapie in Greece. αλληλόμορφα και 23 διαφορετικοί γονότυποι. Ο κυρίαρχος γονότυπος σε όλες τις φυλές ήταν ο ARQ/ARQ, ακολουθούμενος από τον ARR/ARQ. Ο απλότυπος TRQ ήταν συχνός στα Χιώτικα και στα ημίαιμα Χίου πρόβατα, ενώ ο απλότυπος VRQ ήταν σπάνιος σε όλες τις φυλές. Είναι αξιοσημείωτο ότι 3 γονότυποι (ARH/TRQ, ARR/ARK και ARK/VRQ) ανιχνεύθηκαν για πρώτη φορά στην Ελλάδα. Επιπλέον, 2 από αυτούς τους γονότυπους (ARH/TRQ, και ARK/VRQ) δεν έχουν, καθ' όσον γνωρίζουμε, αναφερθεί ποτέ μέχρι σήμερα. Επίσης, δίνεται έμφαση στο γεγονός ότι στη χώρα μας παρατηρούνται τα περισσότερα κρούσματα κλασσικής τρομώδους νόσου των προβάτων κάθε χρόνο σε όλη την Ευρώπη. Συνεπώς είναι επιτακτική ανάγκη να μειωθεί η επίπτωση της νόσου στην Ελλάδα, μέσω της εφαρμογής προγραμμάτων επιλογής/αναπαραγωγής ανθεκτικών προβάτων (selective breeding programs) ως προς την κλασσική τρομώδη νόσο. Σε αυτό συνηγορεί το γεγονός ότι ο επιπολασμός της νόσου στην Ελλάδα συνδέεται με τον γονότυπο ARQ/ARQ, ο οποίος είναι ο πιο συχνός γονότυπος. Άρα η δραστική μείωση του ARQ/ARQ και άλλων ευαίσθητων γονότυπων θα προκαλέσει δραματική μείωση της επίπτωσης της νόσου, προς όφελος της προβατοτροφίας και της εθνικής μας οικονομίας. .Λέξεις-Κλειδιά: τρομώδης νόσος, γονοτυπική ανάλυση, πρωτεϊνη prion, αλυσιδωτή αντίδραση πολυμεράσης

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“…Among breeds, there is a large variation of allelic and genotypic frequencies on the PrP gene (Álvarez et al 2007, 2009aGootwine et al 2008;Lühken et al 2008;Passos et al 2008). In this work, the ARR allele was only detected in the Burkina-Sahel and the Mossi populations.…”

Section: Discussionmentioning

confidence: 54%

“…Polymorphisms at other codons are rare and have not yet been associated with classical scrapie susceptibility. Nowadays, there is an increasing interest to know PrP genotype and allelic frequencies in different sheep populations to carry out association studies with clinical scrapie (Ekateriniadou et al 2007;Lühken et al 2008), ascertain how selection for scrapie resistance can affect selective programmes for performance traits (Alfonso et al 2006) or conservation programmes (Álvarez et al 2007, 2009aWindig et al 2007), and document the variability of PrP gene in areas in which, to date, scrapie cases are few or have not been reported (Gootwine et al 2008;Passos et al 2008;Ün et al 2008) to prepare for possible classical scrapie outbreaks (Kipanyula et al 2009;Álvarez et al 2011). Although many studies have described the genetic susceptibility of scrapie in European sheep breeds, little is known about sheep populations with other origins.…”

Section: Introductionmentioning

confidence: 99%

Prion protein gene polymorphism in four West African sheep populations

Traoré

Royo

Kaboré

et al. 2012

Trop Anim Health Prod

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A total of 162 individuals, belonging to three Burkinabé and one Niger sheep populations, were analysed for prion protein (PrP) gene polymorphism at codons 136, 154 and 171. The ARQ allele was the most frequent in both the Burkinabé (86.7%) and the Niger (67.5%) sheep populations. The highly sensitive allele VRQ was not found in the sampled individuals. The highly resistant ARR allele was in very low frequency in the Burkina-Sahel (4.4%) and Mossi (3.2%) populations and was not present in the Djallonké and Touareg populations. Only 4 out of 15 possible PrP genotypes were identified in the sampled individuals. No favourable ARR/ARR genotypes were found in either of the breeds. Sequencing a subgroup of the samples allowed the identification of other five polymorphisms on the PrP gene sequence at codons 116, 138, 151, 237 and 240. The very low frequency of the ARR allele in the West African sheep should dissuade the implementation of a preventive selection programme aimed to increase resistance to scrapie, to avoid an extreme erosion of the genetic stock.

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Screening for polymorphism at the prion protein (PrP) locus (PRNP) in Awassi and Assaf populations in Israel, the Palestinian Authority and Jordan

Cited by 13 publications

References 9 publications

Prion protein gene (PrP) polymorphisms in healthy sheep in Turkey

Prion protein gene (PrP) polymorphisms in healthy sheep in Turkey

Prion protein gene polymorphisms in classical scrapie-affected flocks of sheep in Central Macedonia

Prion protein gene polymorphism in four West African sheep populations

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