Archaeozoological evidence indicates that sheep were first domesticated in the Fertile Crescent. To search for DNA sequence diversity arising from previously undetected domestication events, this survey examined nine breeds of sheep from modern-day Turkey and Israel. A total of 2027 bp of mitochondrial DNA (mtDNA) sequence from 197 sheep revealed a total of 85 haplotypes and a high level of genetic diversity. Six individuals carried three haplotypes, which clustered separately from the known ovine mtDNA lineages A, B, and C. Analysis of genetic distance, mismatch distribution, and comparisons with wild sheep confirmed that these represent two additional mtDNA lineages denoted D and E. The two haplogroup E sequences were found to link the previously identified groups A and C. The single haplogroup D sequence branched with the eastern mouflon (Ovis orientalis), urial (O. vignei), and argali (O. ammon) sheep. High sequence diversity (K ¼ 1.86%, haplogroup D and O. orientalis) indicates that the wild progenitor of this domestic lineage remains unresolved. The identification in this study of evidence for additional domestication events adds to the emerging view that sheep were recruited from wild populations multiple times in the same way as for other livestock species such as goat, cattle, and pig. A RCHAEOZOOLOGICAL evidence from the ancient Levant points to the Pre-Pottery Neolithic B period, 9000-8000 years ago, as the time when sheep were first herded from the wild, tamed, and domesticated (reviewed by Legge 1996). The form of this wild ancestral population and the number of times and the process of its domestication remain unknown, as does its genetic contribution to the .1400 breeds (Scherf 2000) currently recognized in today's agricultural systems.Mitochondrial sequencing has been used to elucidate the complexity and origins of many modern domestic livestock species, leading to a general theme of multiple maternal lineages. Recent studies of pigs (Larson et al. 2005) et al. 2002). Most recently, a single Karachai animal sampled from the north Caucasus revealed control region sequence, which grouped separately from the three defined ovine mitochondrial DNA (mtDNA) clades (Tapio et al. 2006). This was taken as evidence for a fourth maternal lineage and termed group D.Ovine mitochondrial clade structure and global distribution patterns have been examined using network diagrams generated from collated published control region sequence (Chen et al. 2006;Pereira et al. 2006). These phylogenies, based on a maximum of 531 bp, show clade B to be dominated by animals localized to Europe, clade C by sheep from the Middle East and Asia, and clade A to be a mixture from the Middle East, Asia, and Europe. Generally, these three lineages form starburst clusters, evidence of population expansion, but distant haplotypes are apparent in each group. In clade A, these outliers have been used to suggest group substructure and perhaps a more complicated ovine population history (Chen et al. 2006).The aim of this study wa...

Studies were conducted to determine effects of intrauterine administration of recombinant ovine interferon tau (IFNtau), placental lactogen (PL), and growth hormone (GH) on endometrial function. In the first study, administration of IFNtau to cyclic ewes for one period (Days 11-15) resulted in an interestrous interval (IEI) of approximately 30 days, whereas administration for two periods (Days 11-15 and Days 21-25) extended the IEI to greater than 50 days. Administration of IFNtau from Days 11 to 15 and of PL or GH from Days 21 to 25 failed to extend the IEI more than for IFNtau alone. In the second study, effects of IFNtau, PL, and GH on endometrial differentiation and function were determined in ovariectomized ewes receiving ovarian steroid replacement therapy. Endometrial expression of mRNAs for estrogen receptor (ER), progesterone receptor (PR), and oxytocin receptor (OTR) were not affected by PL or GH treatment; however, uterine milk protein mRNA levels and stratum spongiosum gland density were increased by both PL and GH treatments. Collectively, results indicated that 1) PL and GH do not regulate endometrial PR, ER, and OTR expression or affect corpus luteum life span; 2) down-regulation of epithelial PR expression is requisite for progesterone induction of secretory gene expression in uterine glandular epithelium; 3) effects of PL and GH on endometrial function require IFNtau; and 4) PL and GH regulate endometrial gland proliferation and perhaps differentiated function.

Achromatopsia is a hereditary form of day blindness caused by cone photoreceptor dysfunction. Affected patients suffer from congenital color blindness, photosensitivity, and low visual acuity. Mutations in the CNGA3 gene are a major cause of achromatopsia, and a sheep model of this disease was recently characterized by our group. Here, we report that unilateral subretinal delivery of an adeno-associated virus serotype 5 (AAV5) vector carrying either the mouse or the human intact CNGA3 gene under the control of the red/green opsin promoter results in long-term recovery of visual function in CNGA3-mutant sheep. Treated animals demonstrated shorter maze passage times and a reduced number of collisions with obstacles compared with their pretreatment status, with values close to those of unaffected sheep. This effect was abolished when the treated eye was patched. Electroretinography (ERG) showed marked improvement in cone function. Retinal expression of the transfected human and mouse CNGA3 genes at the mRNA level was shown by polymerase chain reaction (PCR), and cone-specific expression of CNGA3 protein was demonstrated by immunohistochemisrty. The rescue effect has so far been maintained for over 3 years in the first-treated animals, with no obvious ocular or systemic side effects. The results support future application of subretinal AAV5-mediated gene-augmentation therapy in CNGA3 achromatopsia patients.

The Assaf breed of dairy sheep, a stabilized cross of the Awassi and East Friesian breeds, has replaced the Awassi as the breed of choice in its country of origin, Israel, and has spread to other Mediterranean countries. In Israel the Assaf breed is managed under an intensive production system involving weaning lambs at birth, rearing them artificially, and milking ewes after parturition. There are several breeding periods in the year when ewes are mated following hormonally synchronized estrus. Records of 18,976 lactations from 5 farms were analyzed to investigate factors that influenced Assaf milk and reproductive performance. Lactation curves were fitted to each lactation, and a range of parameters and calculated values were analyzed. Daily milk yield records also were analyzed to describe a typical Assaf lactation and compared with those of the Awassi breed. Factors affecting age at first lambing also were studied. An average Assaf ewe kept under this intensive management regimen was found to produce 334 L of milk during a 173-d lactation. Mean litter size was 1.57 lambs/ewe lambing, and lambing interval was 272 d. Milk production was affected by litter size, with twin- and triplet-bearing ewes producing approximately 20 L more milk per lactation than single-bearing ewes. Day length was the major environmental variable influencing milk yield. The difference between midsummer and midwinter day lengths accounted for a difference in daily milk yield of 0.44 L in favor of summer. Ewe lambs that were mated for the first time at later ages produced more lambs and more milk due to greater early lactation characteristics. Milk production was found to be negatively associated with subsequent reproductive performance. Comparing these results with those from an earlier study in the Awassi breed, the Assaf was found to produce less milk during a shorter lactation than the Awassi, but its greater litter size made it a more profitable breed.

No abstract

Regulation of foetal development in sheep depends on interactions between the intrinsic capacity of the foetus for growth and the maternal environment. Lambs born in multi-foetus litters have relatively small placentae with fewer cotelydons, and lower birth weights. Litter-size-dependent intrauterine growth restriction (IUGR) is evident at mid gestation when metabolic needs of the conceptus are moderate, and overnutrition of ewes with multiple foetuses does not promote growth of their foetuses to the size of singletons. Those observations suggest that placental and conceptus growth in multi-foetus pregnancies is reprogrammed at mid gestation by an as yet undefined mechanism to attenuate foetal growth. This may protect the foetus from severe nutritional insult during late gestation, when its daily growth rate is at a maximum. In that way, lambs born in large litters with relatively lower birth weights may not experience the long-term physiological insults that can be observed in small lambs born to undernourished ewes.

Despite milk being an important product from sheep, there are very few reports of milk production from the complete lactation of dairy sheep. The Improved Awassi in Israel is kept under an intensive system of management with lambs being weaned soon after birth. Records from one such flock were analysed to investigate the suitability of various mathematical functions for describing milk yield from the complete lactations of dairy sheep. This included a consideration of whether the functions could cope with short lactations, a characteristic of dairy sheep, and a limited number of test-day records per lactation.Four non-linear mathematical functions were investigated (Wood, Morant, Grossman and Pollott), two of which could also be fitted in a linear and a linear weighted form (Wood and Morant). These functions were fitted to weekly data from a ‘typical Awassi lactation curve’, represented by least squares means of daily milk yield from each week of a 40-week lactation derived from an analysis of 25605 test day records. Characteristics of the lactation were calculated from the functions, such as total milk yield, day and level of peak yield and persistency. These functions were also fitted to 1416 individual lactation records of up to 10 test-day records per lactation. The value of the functions was investigated using the residual mean square (RMS) of the fitted curve as an indicator of how well each function described the lactation. Forms of these functions with a reduced number of parameters were also investigated.The non-linear functions always fitted the data with a lower RMS than their linear equivalent and the weighted form of the linear functions always had a lower RMS than the unweighted form. Of the linear functions, Morant fitted better than Wood. Of the non-linear functions Grossman, Morant and Pollott (additive and multiplicative) fitted the data equally as well but better than Wood. The various functions predicted characteristics of the lactation curve differently; the Wood functions tended to overestimate yield in early lactation and the Morant functions underestimated peak yield.No function was better suited to short lactations than another. However the three-parameter function of Morant, Pollott multiplicative and Pollott additive were considered to be the most suitable for describing the complete lactation of dairy sheep.

This study investigated the factors affecting milk production and lactation curve parameters from the complete lactations of Awassi dairy sheep. The animals were kept in a single flock under intensive management and milked twice daily starting at lambing. Lambs were removed from the ewes at birth into an artificial rearing unit. The results of the analyses of 3740 complete lactations showed a mean litter size of 1·28 lambs born per ewe lambing and average total milk yield of 506 l from lactations 214 days in length and with an average lambing interval of 330 days. Mean lactation number was 3·71 and ewes conceived on average in the 6th month of the lactation. The lactations peaked on day 45 at a yield of 3·44 l. The maximum milk secretion potential of the ewes was 3·9 l/day, with milk yield increasing at 62 g/day mid way between lambing and peak yield and declining at 16·5 g/day mid way between peak and the end of lactation. Age at first lambing, lactation number, litter size, month of lambing and month of conception during the lactation had significant effects (P < 0·05) on some or all of the lactation parameters investigated. Relatively high milk yield was obtained in lactations starting in the January to March period. The monthly effect on milk production was explained by significant (P < 0·05) heat load and photoperiod effects. High milk production was found to have a significant (P < 0·05) adverse effect on reproductive performance. Conception followed a significant (P < 0·05) short term variation in milk production.