Screening for frost tolerance in wheat using the expression of dehydrine genes <i>Wcs120</i> and <i>Wdhn13</i> at 17°C

Holková, Ludmila; Prášil, Ilja Tom; Bradáčová, Marta; Vítámvás, Pavel; Chloupek, O.

doi:10.1111/j.1439-0523.2008.01606.x

Cited by 20 publications

(18 citation statements)

References 19 publications

(17 reference statements)

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“…This set of genotypes was analyzed in three laboratories (CRA-GPG, Fiorenzuola, Italy; Plant Breeding and hardening is associated with the accumulation of COR (cold regulated) proteins. For some of them, for example, COR14 in barley and WCS120 in wheat, a genetic relation between protein accumulation and FT was demonstrated (Fowler et al, 1996;Giorni et al, 1999;Francia et al, 2004;Vítámvás et al, 2007;Kosová et al, 2008;Holková et al, 2009).…”

Section: Methodsmentioning

confidence: 99%

Diversity in the Response to Low Temperature in Representative Barley Genotypes Cultivated in Europe

Rizza¹,

Pagani²,

Gut

et al. 2011

Crop Science

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Barley (Hordeum vulgare L.) production in many regions is constrained by low temperatures. Numerous screening methods have been proposed for studies of freezing tolerance (FT) and winter hardiness (WH). Here, we compared the response to low temperature of 54 barley genotypes released in Europe. Major components of WH were analyzed in fi eld and growth chamber experiments under different hardening and freezing conditions. Phenotype screening of freezing injury (lethal temperature for 50% of the plants [LT50], plant survival, and chlorophyll fluorescence) and indirect evaluations (molecular-marker-based analysis of vernalization requirement) were used. The maximum quantum yield of the photosystem II photochemistry measured as the variable (Fv) to maximal (Fm) fluorescence ratio (Fv:Fm) analysis was confirmed as a reliable method of screening geneticdiversity for FT in plants at early growth stages. Variability for FT was also found after shorter acclimation at optimal (3/1°C day/night) or suboptimal (12/7°C day/night) hardening temperatures. High levels of FT and WH were found in both winter and facultative growth habits. Facultative genotypes were more responsive to early hardening than spring or winter types. Some of them coped best with frost in both laboratory and field experiments. The most tolerant winter genotypes coped best with prolonged exposure to low temperature in the fi eld experiments. A conservative estimation of the role of FT in WH implies that at least 50% of WH was associated with the level of FT. A combined analysis using physiological and molecular tools is proposed for phenotyping WH in large populations

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Section: Methodsmentioning

confidence: 99%

Diversity in the Response to Low Temperature in Representative Barley Genotypes Cultivated in Europe

Rizza¹,

Pagani²,

Gut

et al. 2011

Crop Science

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“…Malz showed the highest expression of both genes after 7 days of stress while in the more tolerant Tadmor it was observed a week later. This finding does not correspond to the generally held notion since more tolerant genotypes exhibit not only a higher expression of stress genes but also an earlier onset of activation of thee genes and a response to lower stress levels [7,13]. Tadmor was repeatedly found to exhibit a later onset of expression of stress genes than other less tolerant varieties.…”

Section:  3 Results and Discussionmentioning

confidence: 59%

“…U odrůdy Malz byly nejvyšší hodnoty exprese obou genů naměřeny po 7 dnech trvání stresu, zatímco u tolerantnější odrůdy Tadmor až o týden později. Tento výsledek neodpovídá obecné představě, protože tolerantnější genotypy vykazují nejen vyšší hladinu exprese stresových genů, ale i dřívější nástup aktivace těchto genů a reakce na nižší hladinu stresu [7,13]. U odrůdy Tadmor zaznamenáváme opakovaně pozdější nástup exprese stresových genů oproti jiným méně tolerantním odrůdám.…”

Section:  3 Výsledky a Diskuseunclassified

Possibility of evaluation of drought tolerance in barley.

Holková¹,

Melišová²,

Bradáčová³

et al. 2010

Kvasny Prum

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Šlechtění odrůd tolerantních vůči suchu je stále důležitější z hlediska zlepšení výnosů a zdravotního stavu rostlin zvláště u hospodářsky významných plodin. Je založeno na hodnocení a účinné selekci tolerantních genotypů. Hodnocení citlivosti rostlin vůči suchu však není jednoduché, vzhledem ke komplexnímu charakteru této vlastnosti. Cílem této práce bylo porovnat stresové reakce pěti různě citlivých genotypů ječmene (Tadmor, Malz, Amulet, Bojos a Jersey) vystavených stresu suchem pomocí molekulárně biologické metody založené na hodnocení aktivity dvou dehydrinových genů (Dhn1 a Dhn4) a ověřit účinnost této metody nejen v laboratorních podmín-kách, ale i v nádobovém pokusu, kdy byla regulována pouze zálivka. Přesnější hodnocení bylo prováděno na rostlinách pěstovaných v kultivačním boxu hydroponicky v podmínkách fyziologického sucha (-0,3 MPa). Transkripční aktivita obou genů byla hodnocena v prů-běhu 14 dnů ve vzorcích z listových tkání v závislosti na citlivosti vůči stresovým podmínkám. U tolerantnějších genotypů (Tadmor a Malz) byla zaznamenána nižší ztráta vody z pletiv a vyšší exprese obou genů. Srovnatelné výsledky pro jednotlivé odrůdy byly získány i v ná-dobovém pokusu. Opět se projevila závislost mezi aktivitou těchto genů a citlivostí odrůd k suchu, zvláště ve fázi metání a plnění zrna. U testovaného souboru odrůd byla dále hodnocena aktivita obou genů v závislosti na působení exogenně aplikovaného fytohormonu ABA. Ukázalo se, že tímto fytohormonem byla aktivována exprese pouze genu Dhn4 a výrazné zvýšení jeho exprese bylo pozorováno pouze u odrůdy Malz. Srovnáním výsledků ze všech tří experimentů jsme ověřili nejen vhodnost použití této metody jako nástroje pro stanovení tolerance ječmene vůči suchu, ale u tolerantnějších odrůd Tadmor a Malz jsme zaznamenali dva odlišné mechanismy aktivace stresových obranných reakcí, které by mohly být způsobeny rozdílnou mírou zapojení fytohormonu ABA v jejich regulaci. Breeding of drought-tolerant varieties, which is steadily gaining in importance for improving crop yields and health condition of economically important crops, is based on evaluation and efficient selection of tolerant genotypes. The assessment of plant sensitivity to drought is not simple owing to the complex character of this trait. We compared the stress responses of five different barley genotypes with different sensitivity (Tadmor, Malz, Amulet, Bojos and Jersey) exposed to drought stress by using a molecular biological method based on evaluating the activity of two dehydrin genes, Dhn1 and Dhn4. The efficiency of the method was verified in laboratory conditions and also in a pot experiment in which only watering was regulated. More detailed evaluation was done on plants grown hydroponically in a cultivation box under conditions of physiological drought (-0.3 MPa). Transcription activity of both genes, as dependent on stress sensitivity, was evaluated over 14 days in leaf tissue samples. The more tolerant genotypes (Tadmor and Malz) exhibited lower water losses from tissues and higher expressio...

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“…The level of expression at low temperatures was related to wheat frost tolerance and the expression at relatively high temperatures of 17°C and 25°C (Holková et al. ). Further, we discriminated barley lines for seed vigour when grown in years suitable for high trait levels and low variation in the trait.…”

Section: Discussionmentioning

confidence: 99%

Use of barley seed vigour to discriminate drought and cold tolerance in crop years with high seed vigour and low trait variation

2013

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Seed vigour is a precondition for early and homogenous field emergence of barley, in addition to effective malting. This study aimed to assess the selection of barley varieties by using seed vigour as the indicator. Seed vigour of barley (quantified as the germination percentage) was evaluated under drought (−0.2 MPa) and temperature stress (10°C). At two locations over a 3‐year period, 1 population of 133 Derkado × B83‐12/21/5 doubled haploid (DH) lines (and parents) was evaluated for seed vigour, of which 108 DH lines were assessed for three malting parameters. The relatively high values of vigour during the 3‐year period (overall average 94–95%) probably impeded high variations in genetic potential. A total of 27 DH lines of the 133 evaluated showed transgression for vigour (up to 98%) in comparison with the parents (Derkado: 96%; B83: 92%). In conclusion, caution should be applied when selecting for seed vigour, even in good crop years with high levels of seed vigour and low trait variations. Such selection might improve vigour, particularly in crop years with unsuitable weather conditions.

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Screening for frost tolerance in wheat using the expression of dehydrine genes Wcs120 and Wdhn13 at 17°C

Cited by 20 publications

References 19 publications

Diversity in the Response to Low Temperature in Representative Barley Genotypes Cultivated in Europe

Diversity in the Response to Low Temperature in Representative Barley Genotypes Cultivated in Europe

Possibility of evaluation of drought tolerance in barley.

Use of barley seed vigour to discriminate drought and cold tolerance in crop years with high seed vigour and low trait variation

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