Screening for Endophytic Fungi from Turmeric Plant (Curcuma longa 
 L.) of Sukabumi and Cibinong with Potency as Antioxidant Compounds Producer

Bustanussalam,; Rachman, Fauzy; Septiana, Eris; Lekatompessy, Sylvia; Widowati, Tiwit; Sukiman, Harmastini; Simanjuntak, Partomuan

doi:10.3923/pjbs.2015.42.45

Cited by 11 publications

(2 citation statements)

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“…The production of bioactive and pharmacologically active or high value compounds through bioconversion/biotransformation processes is known to be green chemistry and has great industrial interest due to its large scale production feasibility (Bérdy 2005; Bustanussalam et al 2015). Endophytic microbes are known to play a pivotal in the biosynthesis of some medicinally important compounds (Ding et al 2006; Wijeratne et al 2006; Lösgen et al 2007; Khumkomkhet et al 2009).…”

Section: Discussionmentioning

confidence: 99%

“…Recently, biotransformation processes have become quite promising due to the large diversity of microbes available and its wide scope in chemical reaction (Chang 2008). However, there are a few limitations associated with the biotransformation such as substrates, enzymes and stability in operation which may also be overcome through synthetic biology approach and exploration of microbial biodiversity (Bérdy 2005; Bustanussalam et al 2015). Endophytes are the bacterial or fungal microorganisms that colonize within the plant tissues (intercellularly and/or intracellularly) without harming the host plants and reported to play a pivotal role in metabolism and biotransformation processes of certain chemical compounds involving in the biosynthetic pathways (Bacon and White 2000; Debbab et al 2009).…”

Section: Introductionmentioning

confidence: 99%

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Bioconversion of curcumin into calebin-A by the endophytic fungus Ovatospora brasiliensis EPE-10 MTCC 25236 associated with Curcuma caesia

Majeed

Thajuddin

et al. 2019

AMB Expr

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Calebin-A is a curcuminoid compound reported to be present in Curcuma longa rhizome. The current study was aimed to isolate and characterize calebin-A from Curcuma caesia rhizome and its production through biotransformation approach using endophytic fungus. C. caesia rhizomes of different ages were subjected to analysis in order to investigate the age at which maximum calebin-A content is present. HP-TLC profiles, HPLC retention times and mass spectrometry detector confirmed the occurrence of calebin-A in C. caesia rhizomes of 12 to 14 months of age but not in rhizomes younger to 12 months. Furthermore, an endophytic fungus strain, EPE-10 that was isolated from the medicinal plant C. caesia was identified as Ovatospora brasiliensis based on morphological and molecular characteristics. This strain O. brasiliensis was deposited to the culture collected centre, MTCC Chandigarh, India under the Budapest treaty and was designated with the Accession Number MTCC 25236. Biotransformation process was carried out at 37 ± 0.5 °C with shaking for 7 days after addition of 0.01% w/v curcumin. Extraction of biotransformed products was done by following partition method and the extracts obtained were analyzed using HPTLC, HPLC and LCMS. The data of the study suggested that O. brasiliensis MTCC 25236 was found to convert curcumin to calebin-A in a time dependant manner with optimum conversion at 48 h. Furthermore, O. brasiliensis MTCC 25236 was found to be positive for the Baeyer–Villiger monooxygenase (BVMOs) enzyme activity which could possibly be the mechanism of this bioconversion. The results of this study for the first time indicated that the endophytic fungus identified as O. brasiliensis MTCC 25236 isolated from the C. caesia rhizome could be a possible source for naturally producing calebin-A.

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