Screening differentially expressed proteins of coronary heart disease with congenital cold syndrome based on tandem mass tag (TMT) technology

Abstract

In our previous studies, we discovered the congenital cold syndrome (CCS), which is characterized by ‘qi deficiency and qi stagnation, mixed cold and heat.’ And there is a type of syndrome with special incidence characteristic. However, the diagnosis of CCS still lacks an objective basis. In this study, we performed Tandem Mass Tag (TMT) based on quantitative proteomics technology to screen the significantly differentially expressed proteins (DEPs) in serum of patients with coronary heart disease (CHD) patients with CCS, patients with heart and kidney yang deficiency, and healthy people. A total of 22 DEPs (nine upregulated and 13 downregulated) were identified between patients with CCS and healthy subjects. Next, we performed GO and KEGG pathway enrichment analysis, we found the primary functions of DEPs of CCS were binding, catalytic activity, and molecular function regulator. These DEPs were mainly involved in important biological processes, such as cellular process, response to stimulus, localization, metabolic process, and biological regulation. The KEGG analysis revealed that the DEPs showed significant changes in fructose and mannose metabolism, Pentose phosphate pathway, and Arrhythmogenic right ventricular cardiomyopathy. After parallel reaction monitoring (PRM) verification, four upregulated target proteins (ALDOA, PCYOX1, Crisp3 and IGLV4-69) and three downregulated proteins (ALDOC, ADAMTSL-2 and C3) were accurately identified. These proteins were mainly related to immune response and glucose metabolism. These DEPs could be the marker proteins of coronary heart disease with CCS. This findings help to reveal the pathogenesis of CHD with CCS and provide potential therapeutic targets.

Multi-gene models for survival prognosis of colorectal cancer patients

“…IGLV4-69 was predicted to be associated with an immune response, and it is thought that IGLV4-69 is part of the immunoglobulin complex and is active extracellularly [41] . Screening for differentially expressed proteins in coronary artery disease congenital cold syndrome based on tandem mass-labeling techniques revealed upregulated expression of IGLV4-69 [42] . IGSF9 is active in axons and plasma membranes and plays a role upstream of dendrite development or in the regulation of dendrite organization [43] .…”

Lysophosphatidylcholine promotes intercellular adhesion molecule-1 and vascular cell adhesion molecule-1 expression in human umbilical vein endothelial cells via an orphan G protein receptor 2-mediated signaling pathway

“…Levels of LDL particles, which are composed of triglycerides, phospholipids, and cholesterol, are directly associated with the severity of atherosclerotic disease. While passive LDL retention was originally thought to drive the progression of atherosclerosis, more recent research has instead shown that LDL can undergo an array of oxidative modifications within the vasculature including esterification and lipid peroxidation, yielding oxLDL [ 57 ]. In addition to being potentially antigenic, oxLDL can directly promote inflammation and the activation of innate and adaptive immune responses [57].…”

“…Atherosclerosis is currently the leading cause of death throughout the world [ 1–4 ]. While elevated overall cholesterol levels have long been known to be associated with the risk of atherosclerotic disease, there is also growing evidence that low-density lipoprotein (LDL) oxidation is closely related to this pathogenic condition [ 5–7 ]. While atherosclerosis and related diseases can exhibit a diverse array of clinical and pathological manifestations, inflammation is a hallmark of all forms of these diseases, and a number of bioactive lipid mediators have been linked to these inflammatory processes [ 8–10 ].…”

ADAMTS Proteins and Vascular Remodeling in Aortic Aneurysms

“…Adamtsl2 was found to be overexpressed in microvascular endothelial cells of ventricles in a murine model of transverse aortic constriction [ 72 ]. Using mass spectroscopy on healthy hearts and hearts presenting coronary diseases, ADAMTSL2 was found to be under-expressed in the unhealthy heart [ 73 ].…”