Screening Concepts for the Isolation of Biosurfactant Producing Microorganisms

Walter, Vanessa; Syldatk, Christoph; Hausmann, Rudolf

doi:10.1007/978-1-4419-5979-9_1

Cited by 244 publications

(210 citation statements)

References 51 publications

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“…Although biosurfactant-producing strains are most often retrieved from hydrocarbon enrichment cultures [74], this capacity has been frequently found among surfactant-resistant isolates [20,54]. A higher fraction of biosurfactant-producing strains could probably be achieved with different culture conditions that, in the present case, were more directed to select for surfactant resistance.…”

Section: Isolation Of Surfactant-resistant Bacteriamentioning

confidence: 77%

“…Biosurfactant production is often associated with the presence of hydrophobic substrates, because it plays a natural role in enhancing their bioavailability [58]. However, hydrophobic substrates are not a prerequisite for biosurfactant production [74], and biosurfactant-producing microorganisms have been isolated with other carbon sources, namely, Zobell medium [21], nutrient agar [1], minimal medium with glucose [13,25], and PY medium amended with synthetic surfactants [54]. Some of the attempts to use hydrophobic carbon sources in selective or enrichment media have also achieved modest percentages of positive results, despite intense screening efforts: less than 10% of the isolates from oil-contaminated soils [48,72,77], 4% from oil-spilled seawater [46], and 9.2% from terrestrial and marine samples [7].…”

Section: Isolation Of Surfactant-resistant Bacteriamentioning

confidence: 99%

See 1 more Smart Citation

Isolation of Surfactant-Resistant Pseudomonads from the Estuarine Surface Microlayer

Louvado

Coelho²,

Domingues³

et al. 2012

J. Microbiol. Biotechnol.

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Section: Isolation Of Surfactant-resistant Bacteriamentioning

confidence: 77%

Section: Isolation Of Surfactant-resistant Bacteriamentioning

confidence: 99%

Isolation of Surfactant-Resistant Pseudomonads from the Estuarine Surface Microlayer

Louvado

Coelho²,

Domingues³

et al. 2012

J. Microbiol. Biotechnol.

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“…In terms of screening the activity of biosurfactant produced by bacteria, the principle aim is to find new compounds with strong interfacial activity, low critical micelle concentration (CMC), high emulsion capacity, good solubility and activity in a broad-pH range [34]. The biosurfactantproducing bacteria need to be easily cultivated and producing high yield of biosurfactant to ensure economical and sustainable commercialization.…”

Section: Discussionmentioning

confidence: 99%

Isolation and Characterization of Biosurfactant-Producing Bacteria Isolated From Petroleum Contaminated Sites With the Potential to Be Used in Bioremediation

Zainal¹,

Omar²,

Ashaari³

2017

Sci. herit. j.

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Surfactants have been widely used to facilitate biodegradation of petroleum pollutant; particularly those are being produced by microorganisms which are also known as biosurfactant. Six bacterial isolates from petroleum contaminated sites were found to be potential hydrocarbon degrader candidates based on their ability to grow on minimal media supplemented with petroleum crude oil as sole carbon source. In addition, they also found to be having potential in producing extracellular biosurfactant based on the results of screening for biosurfactant activity. These isolates were further identified and characterized. Morphological and biochemical characteristics of these isolates have been examined and molecular identification was done by amplifying 16S rRNA gene. Three isolates were identified as the member of the genus Pseudomonas; another two isolates were the member of genus Comamonas and one isolate from the genus Stenotrophomonas. Several qualitative screening methods (microplate assay, oil displacement test, emulsification assay and drop-collapse test) showed variation of results; suggesting the need to support these findings with quantitative screening based on measurement of surface activity. BSP 6 which was found to be closely related to Comamonas aquatica was the most promising biosurfactant producer found in this study based on two positive results out of four qualitative screening methods performed.

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“…Many researchers have been previously reported about the significance of multiple screening tests for the isolation of most potential biosurfactant producers and chosen methods were standard techniques for the effective isolation of biosurfactant producer (Mani et al, 2016a;Khopade et al 2012;Kiran et al 2009). Similarly, Walter et al, 2010 described that the combination of different methods is appropriate for a successful screening of biosurfactant producing bacteria.…”

Section: Discussionmentioning

confidence: 99%

A glycolipid biosurfactant from a native halophilic Bacillus species of Porto Novo, India and its potential microbially enhanced oil recovery properties

2016

Int. J. Adv. Res. Biol. Sci.

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A potential biosurfactant producing halophilic bacterium was isolated from the saltpan environment of Porto Novo, Tamilnadu, India. The potential bacterium showed 69.9 % emulsification activity, 56.7 units/ml lipase activity and 22 mm oil displacement in the biosurfactant multiple screening methods. This halophilic bacterium was identified as Bacillus species using 16S rRNA molecular sequencing method. This strain showed growth dependent biosurfactant production, which was partially purified and weighed at 0.945 ±0.021 g/L on dry weight basis. The isolated biosurfactant was biochemically identified as glycolipid using biochemical methods and FT-IR functional group analysis. Further, the glycolipid surfactant showed consistent oil recovery efficiency than the tested synthetic surfactant at the temperature up to 50°C and pH ranged from 5 to 8. Based on these observations, the isolated biosurfactant using this halophilic bacterium revealed promising microbially enhanced oil recovery and bioremediation purposes at a wide range of environmental conditions.

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Screening Concepts for the Isolation of Biosurfactant Producing Microorganisms

Cited by 244 publications

References 51 publications

Isolation of Surfactant-Resistant Pseudomonads from the Estuarine Surface Microlayer

Isolation of Surfactant-Resistant Pseudomonads from the Estuarine Surface Microlayer

Isolation and Characterization of Biosurfactant-Producing Bacteria Isolated From Petroleum Contaminated Sites With the Potential to Be Used in Bioremediation

A glycolipid biosurfactant from a native halophilic Bacillus species of Porto Novo, India and its potential microbially enhanced oil recovery properties

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