Screening bioactive compounds from natural product and its preparations using capillary electrophoresis

Abstract

Screening bioactive compounds from natural product and its preparations using capillary electrophoresisBioactive ingredients of natural products can protect human body from harm, as well as prevent and treat disease. Screening bioactive compounds from natural products is attracting particular attention. It is a great challenge to separate and detect the active compounds from complex matrix natural products. CE plays a vital role in screening active compounds because of its unique features such as high-efficiency separation, short-analysis time, minimal sample consumption, and ease to realize automatization etc. Additionally, CE has been developed various modes owing to its abundant advantages in analysis and separation of compounds. The purpose of this work is to review previous developments and applications of CE in screening bioactive compounds derived from natural products from 2007 to 2017. This review does not only summarize the traditional methods of detecting active ingredients but also briefly introduces some novel assays. The trends in the application of CE in active compounds screening are addressed in the article.

Antioxidant Activity of Flavonoid Rich Fraction of Ligaria cuneifolia (Loranthaceae)

“…It has been widely applied to plant analysis due to its excellent efficiency, short analysis time, minute sample requirements, and use of few organic reagents. [20,22] The biological activity screening technology is a method that combines biological activity test with separation of chemical compounds from a complex matrix and capillary electrophoresis proved to be an efficient tool for the screening of bioactive components. In this case, we applied a CD-MEKC methodology in precolumn mode where there is a reaction between DPPH and active ingredients followed by analysis and detection using capillary electrophoresis.…”

“…In this case, we applied a CD-MEKC methodology in precolumn mode where there is a reaction between DPPH and active ingredients followed by analysis and detection using capillary electrophoresis. [22] Given the complex nature of plant extracts and the coexistence of a large number of chemicals that might exert an antioxidant effect with variable efficiency, it is critical to identify the most active ones under different experimental conditions. Moreover, instead of studying isolated antioxidant compounds, it is also important to consider this effect in the context of the whole plant extract, where synergy and/or antagonism might occur.…”

Modern separation techniques coupled to high performance mass spectrometry for glycolipid analysis

“…As a microfluidic technique, CE realizes the separation and electromigration of the analytes in narrow‐bore capillaries by the application of high electric fields, up to 0.6 kV/cm; hence, a remarkable efficiency of the separation can be achieved. Besides, CE is highly recommended for the screening of biologically active compounds because of its unsurpassed sensitivity, short‐analysis time, reduced sample consumption, and the compatibility with automation and miniaturization . The utility of CE towards glycolipid profiling and identification can be greatly enhanced by ESI MS detection since by combined CE ESI MS, not only molecular masses of the separated species can be directly measured, but also specific fragment ions may be generated by MS/MS from individual components in a complex mixture, to deduce the structure of the lipid and the attached glycan moiety.…”

Herbal medicines on the spot: back to the green essentials

“…This provides an additional separation mechanism, based on analyte partition in the micelle and electromigration. 21,22 An example of the latter is related to the analysis of Ligaria cuneifolia extracts by a novel dual cyclodextrin MEKC-DAD methodology. 23 Microemulsion electrokinetic chromatography (MEEKC) applies microemulsions as the background electrolyte to improve the solubility of highly lipophilic compounds.…”