Screening and selection of new microbial anti-Candida

Moussaid, Fatimazahrae; Barnossi, Azeddin El; Chahmi, Naima; Housseini, Abdelilah Iraqi

doi:10.1016/j.matpr.2019.04.070

Cited by 2 publications

(7 citation statements)

References 14 publications

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“…Neutral pH is the optimal pH of the production of antifungal metabolites of our isolate, this is in line with the studies of Bamidele et al [28]which have shown that the neutral pH gives the best antifungal activity against C. albicans. The temperature of 30°C is the optimal temperature for the production of antifungal metabolites of Gn-A11-18 isolate against C. albicans and A. niger, our results are in accordance with those of [19,25]which showed that the optimal temperature of antifungal activity is 30°C, and as opposed to Bamidele et al [28]which showed in their studies that the temperature 37°C is the optimal temperature for the production of antifungal substances from lactic acid bacteria to control C. albicans.…”

Section: In Vitro Inhibitory Effects Of Gn-a11-18 Isolatesupporting

confidence: 92%

“…The inoculated petri dishes have been incubated at 30°C in the dark and in an atmosphere saturated with moisture. The inhibition diameter and inhibition percentage have been determined after 48 hrs and 7 days of incubation for C. albicans and A. niger respectively [25,26].…”

Section: Agar Plug Diffusion Methodsmentioning

confidence: 99%

“…Petri dishes containing the MEA medium have been sown with C. albicans and inoculated with A. niger. Wattman paper disks (6 mm in diameter) have been deposited on the surface of the inoculated media and impregnated with 20 μl filtrate [25]and wells (6 mm in diameter) have been made by a sterile die on the inoculated media, then filled with 100 µl filtrate [28]. The inoculated petri dishes have been incubated at 30°C in the dark.…”

Section: Disk and Well Diffusion Methodsmentioning

confidence: 99%

“…The results obtained are shown in figure (4) and (Table. 2), which shows that the bioactive substance which has been present antifungal activity of the Gn-A11-18 isolate is thermoresistant. Several scientific research studies have focused on the control of C. abicans and A. niger by the use of essential oils and substances of bacterial and fungal origin including Moussaid et al [25], which have shown the isolation of an F27 isolate, the filtrate of this isolate has an antifungal activity with an inhibition diameter of 14.7 mm against C. albicans.Balouiri et al [19]have been shown in this research that Bacillus spp isolated from Calotropis Procera Ait rhizosphere has shown an antifungal activity against C. albicans ATCC 102031 with an inhibition diameter of 36.33 mm on the YM (Yeast and Malt extract) medium. The study by Bamidele et al [28]showed that the six strains of lactic acid bacteria showed varying degrees of anti-Candida activity, especially Pediatric pentosaceus BTA 51 cucumber showed the largest inhibition zone of 14 mm at neutral pH.…”

Section: In Vitro Inhibitory Effects Of Gn-a11-18 Isolatementioning

confidence: 99%

“…1N and H 3 PO 4 1N, growth at different temperatures(25,30, 37, 44 and 50 °C) and growth in the NB medium containing different NaCl concentrations (0, 2, 4, 6, 8 and 10% (w/v)) have also been tested[30].2.6. Statistics analysisAll tests and experiments have been repeated three times.…”

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confidence: 99%

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Antifungal activity of Bacillussp. Gn-A11-18isolated from decomposing solid green household waste in water and soil against Candida albicans and Aspergillus niger

2020

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Candida albicans an opportunistic pathogenic fungus causes many infections in humans. Whereas Aspergillus niger is a fungus that can produce ochratoxins, a group of extremely dangerous secondary metabolites that are classified as potentially carcinogenic to humans and also causing deterioration in grapes, strawberries, etc. The purpose of the current study is to isolate, purify, identify and characterize new microorganisms associated with solid green household waste for the control of C. albicans and A. niger. The antifungal activity of bacterial isolates was carried out in vitro by the agar plug diffusion method, the disk and well diffusion method. The isolate that showed promising activity has been identified by those macroscopic, microscopic and biochemical characteristics. The results obtained in the course of this study showed the isolation of an isolate named Gn-A11-18, which was shown to have significant inhibitory activity with a 42.66% inhibition percentage against A. niger and a 44.66 mm inhibition diameter against C. albicans compared to the controls. The identification of Gn-A11-18 isolate has shown that this isolate belongs to the genus Bacillus with a similarity to Bacillus subtilis and Bacillus tequilensis. In the light of the results of this study, we can suggest that the bioactive compound of Bacillus sp. Gn-A11-18 could become a biological alternate that could have an important role to fight against C. albicans and A. niger.

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Section: In Vitro Inhibitory Effects Of Gn-a11-18 Isolatesupporting

confidence: 92%

Section: Agar Plug Diffusion Methodsmentioning

confidence: 99%

Section: Disk and Well Diffusion Methodsmentioning

confidence: 99%

Section: In Vitro Inhibitory Effects Of Gn-a11-18 Isolatementioning

confidence: 99%

mentioning

confidence: 99%

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Antifungal activity of Bacillussp. Gn-A11-18isolated from decomposing solid green household waste in water and soil against Candida albicans and Aspergillus niger

2020

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SALURAN PENCERNAAN UDANG JERBUNG, Penaeus merguiensis

Mulianto

Widyowati²,

Raharja³

et al. 2022

Jurnal Riset Akuakultur

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Upaya untuk meningkatkan pemanfaatan protein dalam pakan dapat dilakukan dengan penambahan bakteri proteolitik. Penelitian ini bertujuan untuk skrining bakteri proteolitik pada saluran pencernaan udang Jerbung (Penaeus merguiensis) sebagai kandidat probotik. Skrining bakteri dilakukan dengan mengisolasi bakteri dari saluran pencernaan udang yang diperoleh dari tangkapan nelayan di pesisir pantai Pasuruan, Jawa Timur. Dari hasil isolasi diperoleh lebih dari 30 koloni yang tumbuh pada media, selanjutnya dipilih lima isolat yang dengan karakteristik morfologi yang berbeda. Lima isolat terpilih selanjutnya diuji aktivitas proteolitiknya pada media skim milk agar (SMA). Hasil penelitian menunjukkan aktivitas proteolitik terbesar pada isolat UD-1 dengan nilai aktivitas proteolitik sebesar 2,49 ± 0,9 cm; UD-2 sebesar 2,33± 0,24 cm; UD-3 sebesar 1,85 ± 0,07 cm; UD-4 sebesar 1,11 ± 0,43 cm; dan UD-5 sebesar 1,36 ± 0,07 cm. Tiga isolat dengan nilai aktivitas proteolitik tertinggi kemudian diuji pewarnaan gram, uji ketahanan terhadap kondisi asam (pH 3), uji penempelan bakteri, uji antagonistik terhadap bakteri Vibrio harveyi, uji patogenitas dan pengamatan pertumbuhan bakteri. Hasil uji pewarnaan gram dengan hasil gram positif pada ketiga isolat. Ketiga isolat mampu bertahan hidup pada kondisi asam (pH 3) selama delapan jam dengan nilai kepadatan (OD ë 620 nm) pada isolat tertinggi UD-1 (0,875). Uji antagonistic menunjukkan isolat UD-1, UD-2, dan UD-3 mampu menghambat pertumbuhan bakteri Vibrio harveyi dengan membentuk zona hambat di sekeliling isolat. Nilai antagonistik tertinggi pada isolat UD-1 sebesar 12,3 mm. Uji patogenitas yang dilakukan menunjukkan bahwa isolat tidak bersifat patogen pada udang budidaya. Berdasarkan hasil penelitian dapat disimpulkan bahwa isolat (UD-1, UD-2, dan UD3) yang diisolasi dari saluran pencernaan udang termasuk bakteri proteolitik dan memenuhi syarat sebagai bakteri kandidat probiotik.Efforts to increase the utilization of protein in feed can be made by adding proteolytic bacteria. This study aimed to screen proteolytic bacteria in the digestive tract of Banana shrimp (Penaeus merguiensis) as probiotic candidates. The bacterial screening was carried out by isolating bacteria from the digestive tract of shrimp obtained from fishermen’s catch on the coast of Pasuruan, East Java. From the isolation results, more than 30 colonies grew on the media, then five isolates were selected with different morphological characteristics. The five selected isolates were then tested for their proteolytic activity on Skim Milk Agar (SMA) media. The results showed the most excellent proteolytic activity in isolate UD-1 with a proteolytic activity value of 2.49 ± 0.9 cm; UD-2 is 2.33 ± 0.24 cm; UD-3 is 1.85 ± 0.07 cm; UD-4 is 1.11 ± 0.43 cm; and UD-5 is 1.36 ± 0.07 cm. The three isolates with the highest proteolytic activity values were tested for gram-positive staining, acid resistance test (pH 3), bacterial attachment test, antagonistic test against Vibrio harveyi bacteria, pathogenicity test, and bacterial growth observation. Gram stain test results with gram-positive results on the three isolates. The three isolates were able to survive in acidic conditions (pH 3) for eight hours with the highest density (OD ë 620 nm) value of UD-1 (0.875). The antagonistic test showed that isolates UD-1, UD-2, and UD-3 could inhibit the growth of Vibrio harveyi bacteria by forming an inhibitory zone around the isolates. The highest antagonistic value in the UD-1 isolate was 12.3 mm. The pathogenicity test carried out showed that the isolates were not pathogenic in cultured shrimp. Based on the study results, it can be concluded that the isolates (UD-1, UD-2, and UD-3) isolated from the shrimp’s digestive tract are proteolytic bacteria and qualify as probiotic candidate bacteria.

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Screening and selection of new microbial anti-Candida

Cited by 2 publications

References 14 publications

Antifungal activity of Bacillussp. Gn-A11-18isolated from decomposing solid green household waste in water and soil against Candida albicans and Aspergillus niger

Antifungal activity of Bacillussp. Gn-A11-18isolated from decomposing solid green household waste in water and soil against Candida albicans and Aspergillus niger

SALURAN PENCERNAAN UDANG JERBUNG, Penaeus merguiensis

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