Antifungal activity of Bacillussp. Gn-A11-18isolated from decomposing solid green household waste in water and soil against Candida albicans and Aspergillus niger

Abstract

Candida albicans an opportunistic pathogenic fungus causes many infections in humans. Whereas Aspergillus niger is a fungus that can produce ochratoxins, a group of extremely dangerous secondary metabolites that are classified as potentially carcinogenic to humans and also causing deterioration in grapes, strawberries, etc. The purpose of the current study is to isolate, purify, identify and characterize new microorganisms associated with solid green household waste for the control of C. albicans and A. niger. The antifungal activity of bacterial isolates was carried out in vitro by the agar plug diffusion method, the disk and well diffusion method. The isolate that showed promising activity has been identified by those macroscopic, microscopic and biochemical characteristics. The results obtained in the course of this study showed the isolation of an isolate named Gn-A11-18, which was shown to have significant inhibitory activity with a 42.66% inhibition percentage against A. niger and a 44.66 mm inhibition diameter against C. albicans compared to the controls. The identification of Gn-A11-18 isolate has shown that this isolate belongs to the genus Bacillus with a similarity to Bacillus subtilis and Bacillus tequilensis. In the light of the results of this study, we can suggest that the bioactive compound of Bacillus sp. Gn-A11-18 could become a biological alternate that could have an important role to fight against C. albicans and A. niger.

Chemical Analysis and Antioxidant and Antimicrobial Activity of Essential oils from Artemisia negrei L. against Drug-Resistant Microbes

“…These results are in agreement with investigated elsewhere [ 45 ], which showed that oil from Borojoa patinoi Cuatrec exhibited an inhibitory effect towards C. albicans since both share some common chemicals. Moreover, numerous studies place priority natural products especially essential oil for controlling fungal strains including F. oxysporum , A. niger , A. flavus, and C. albicans [ 26 ]. Several epidemiological studies have been conducted on yeast infections indicated that Candida is responsible for many diseases [ 46 ].…”

“…Next, Whatman paper disks (6 mm in diameter) impregnated with 10 μ L of essential oils were placed on the surface of Petri dishes before being incubated at 30°C in the darkness. The inhibition diameter, as well as inhibition percentage, was determined after 48 h of incubation for C. albicans and after 7 days for A. niger, A. flavus, and F. oxysporum [ 26 , 27 ].…”

Tangerine, banana and pomegranate peels valorisation for sustainable environment: A review

“…TPW, PPW and BPW have a relatively high microbial biodiversity [ 90 , 283 ]. Over the last few years, numerous research studies have focused on the recovery of microorganisms isolated from these wastes in the medical, agronomic and industrial fields.…”

“…Also, Maeno et al [ 289 ] have isolated Pseudofructophilic Leuconostoc citreum Strain F192-5 from TPW able to produce mainly lactate and acetate, with trace amounts of ethanol, from d -glucose metabolism. For PPW, El Barnossi et al [ 283 ] have isolated and identified a Bacillus sp. strain Gn-A11-18 from pomegranate waste that has shown a significant antifungal activity against Candida albicans and A. niger .…”

Wild Chamomile (Matricaria recutita L) from the Taounate Province, Morocco: Extraction and Valorisation of the Antibacterial Activity of its Essential Oils

“…Wattman paper disks (6 mm in diameter) were deposited on the surface of the inoculated media and impregnated with 20 μL EOs extracted from M. recutita. 22 The inoculated petri dishes were incubated at 37 °C in the dark. The inhibition diameter was determined after 24 hrs of incubation.…”