Screening and quantification of anticancer compounds in traditional Chinese medicine by hollow fiber cell fishing and hollow fiber liquid/solid‐phase microextraction

Self Cite

A novel stirrer-liquid/solid microextraction method was developed for the separation and enrichment of trace levels of curcumin, bisdemethoxycurcumin, and demethoxycurcumin in Rhizoma Curcumae Longae, Radix Curcumae, and Rhizoma Curcumae before their analysis by high-performance liquid chromatography with ultraviolet detection. In the proposed approach, a magnetic stirrer was immersed in decanol to coat its surface completely with decanol, which was used as an extraction platform. The stirrer coated with decanol is not only a power to agitate the sample solution to constantly update the sample on the stirrer surface but also it can adsorb and extract the target analytes. Some effective parameters, including suitable superficial area of stirrer, extraction solvent, sample phase pH, NaCl concentration, stirring rate, extraction time, sample phase volume, were analyzed and selected. Under the optimal conditions, the linearities are 0.0044-2.20 μg/mL, detection limits are 0.3-0.6 ng/mL, and the extraction content per unit length and enrichment factors of the target analytes are 6.24-9.71/mm and 589-917, respectively. Also, the stirrer-liquid/solid microextraction mechanism for the extraction and enrichment of the target analytes was analyzed and expounded. The results showed that stirrer-liquid/solid microextraction is a simple, rapid sample pretreatment approach with a high enrichment factor.

Section: Resultsmentioning

confidence: 99%

“…Over the past years, a wide range of pharmacological activities of curcuminoids have been extensively studied, such as anti‐inflammatory , anti‐oxidant , anti‐cancer , anti‐viral, and wound healing . The trace levels of curcumin, DMC and BDMC quantified simultaneously in traditional Chinese medicine (TCM) are rarely reported .…”

Section: Introductionmentioning

confidence: 99%

Development of a novel stirrerliquid/solid microextraction method for the separation and enrichment of trace levels of active compounds in traditional Chinese medicine

Wang

Chen

et al. 2016

Self Cite

“…Wang et al. used the hollow fiber liquid/solid‐phase microextraction with salt in sample phase for extraction of the phenolic acids in TCMs.…”

Section: Introductionmentioning

confidence: 99%

Ballpoint connector‐protected salt‐oil‐salt liquid phase microextraction for concentration and enrichment of trace anthraquinone compounds in rhubarb

Chen

et al. 2019

Self Cite

This study proposed a new ballpoint connector-protected salt-oil-salt liquid phase microextraction for extraction and enrichment of trace rhein and chrysophanol in rhubarb prior to determination of the analytes by high performance liquid chromatography. In this study, a handy ballpoint connector (between ballpoint tip and ink chamber) was used as extraction device, in which its cavity was filled with n-octanol, and the bare n-octanol in its two opening ends was covered with a thin layer of sodium chloride film. The design subtly assembled salt film onto ballpoint connector for extraction and enrichment, which greatly improved the enrichment factors of the target analytes. Moreover, the novel procedure and its extraction mechanism were described and analyzed, and several crucial parameters reflecting the extraction effect were investigated and optimized. Under optimum conditions, high enrichment factors (247 and 127), good linearities with r ≥ 0.9998, limits of detection (0.6-1.1 ng/mL), relative standard deviations of intra-and interday (2.2-8.8% and 4.3-8.9%), and average recoveries (97.6-98.1%), were obtained, respectively. The proposed method can not only eliminate the negative effects from viscosity and ion strength at high salt concentration of sample phase, but also make salting-out effect be focused on small area so as to maximize the extraction effect.

“…Thus, the development of sensitive and specific analytical procedure for the separation and quantification of TAM is necessary. Several sample pretreatment techniques such as SPE , online extraction , hollow fiber/solid‐phase microextraction and SPE have been reported for isolation and preconcentration of TAM from different matrices. Among these techniques, SPE is a common surface‐dependent method for preconcentration and purification of TAM.…”

Section: Introductionmentioning

confidence: 99%

Electrospun polyethylene terephthalate/graphene oxide nanofibrous membrane followed by HPLC for the separation and determination of tamoxifen in human blood plasma

Sereshti

Bakhtiari

Najarzadekan

et al. 2017

An electrospun polyethylene terephthalate/graphene oxide nanofibrous mat was fabricated and used as an effective and novel membrane for the solid-phase extraction of tamoxifen in human blood plasma samples before detection by high-performance liquid chromatography. The membrane was characterized by some identification techniques, such as FTIR spectroscopy, X-ray diffraction, and scanning electron microscopy. The effective variables of the extraction procedure including desorption condition (type and volume of the eluent), adsorbent dose, pH of sample solution, salt concentration, and sample loading time were investigated and their optimum values were obtained using one factor at a time methodology. Under the optimized conditions, the results showed wide linear concentration range of 5-2000 ng/mL with a determination coefficient of 0.992. The limits of detection and limits of quantification were 1.3 and 5.0 ng/mL, respectively. The intra-day and inter-day precisions were 3.4 and 4.6%, respectively. The method was successfully applied to determination of tamoxifen in the blood plasma samples and satisfactory relative recoveries (92.6-98.3 %) were achieved.