Screening and Optimization of Newly Isolated Thermotolerant Lysinibacillus fusiformis Strain SK for Protease and Antifungal Activity

Khadka, Sujan; Adhikari, Sanjib; Thapa, Alina; Panday, Raju; Adhikari, Manjila; Regmi, Ramesh Sharma; Adhikari, Namita Paudel; Proshad, Ram; Koirala, Niranjan

doi:10.1007/s00284-020-01976-7

Cited by 10 publications

(13 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Gram-positive, catalase-positive, urea-positive and inability to hydrolyse starch are the biochemical characteristics of Lysinibacillus sphaericus reported in the study. These characteristics were also reported by [ 26 ].…”

Section: Discussionsupporting

confidence: 86%

See 1 more Smart Citation

In vitro antibacterial activities and molecular characterization of bacterial species isolated from farmlands against selected pathogens

Onajobi

Idowu

Adeyemi

et al. 2020

Biotechnology Reports

View full text Add to dashboard Cite

Highlights Screening bacterial isolates for antagonistic potential. Molecular methods for identification of isolates. Six potent antibacterial isolates against the test pathogenic species. Pseudomonas was susceptible to antagonizing strains. Necessity for new effective antibacterial formulation.

show abstract

Section: Discussionsupporting

confidence: 86%

“…A series of dilution were carried out in order to obtain numerable number of colony [ 26 ]. These dilutions were evenly spread on the solidified nutrient agar (NA) and incubated for 2 days at 30 °C.…”

Section: Methodsmentioning

confidence: 99%

In vitro antibacterial activities and molecular characterization of bacterial species isolated from farmlands against selected pathogens

Onajobi

Idowu

Adeyemi

et al. 2020

Biotechnology Reports

View full text Add to dashboard Cite

show abstract

“…Colonies obtained were subjected to Gram's staining and relevant biochemical tests (catalase, oxidase, Voges–Proskauer, gelatin hydrolysis, casein hydrolysis, citrate utilization, and sugars fermentation) for confirmation of Bacillus sp. [ 8 ]. For the extraction of genomic DNA from the isolates, the cells were boiled for 15 min before being centrifuged at 10,000 rpm for 20 min at 4 °C.…”

Section: Methodsmentioning

confidence: 99%

“…is one of the most investigated microbial groups because they can produce varieties of biotechnological applicable substances such as γ–PGA, amylase, protease, etc. [ 6 , 7 , 8 , 9 ].…”

Section: Introductionmentioning

confidence: 99%

Screening and characterization of potent poly glutamic acid producing Bacillus sp. isolated from Kinema, water and soil samples

Thapa

Khadka

et al. 2021

Heliyon

Self Cite

View full text Add to dashboard Cite

Microbially produced gamma poly glutamic acid (γ–PGA) is a commercially important biopolymer with many applications in foods and various other substances and are abundantly used in different parts of the world. With an aim to study the potent γ–PGA producing Bacillus species, a total of 47 different samples ( Kinema , soil, and water) were randomly collected from different locations across the country, and Bacillus sp. were selectively isolated, screened, and characterized by performing physiological, biochemical, morphological, and 16S rRNA gene sequencing. The microbial production of γ–PGA was assayed with the selected isolates on the PGA medium and the metabolite obtained was recovered by ethanol precipitation method and further characterized by thin-layer chromatography (TLC). Thermotolerance (25–60 °C), pH tolerance (4–9), and NaCl tolerance (1–9%) tests were performed to optimize the bacterial growth and γ–PGA production and its viscosity were measured by Ostwald's viscometer. Out of 145 randomly selected colonies, 63 isolates were Gram-positive, rods, and endospore producers and were presumptively confirmed as genus Bacillus. Higher growth of γ–PGA producers were reported in 22 isolates and was found at optimum conditions such as temperature (30–37 °C), pH (6.5–7), incubation time (3 days), and NaCl concentration (3%) and γ–PGA thus produced was further verified by TLC with the retention factor (RF) value 0.27. The potent isolates were closely similar to Bacillus subtilis subsp . stercoris , Bacillus cereus , Bacillus paranthracis, and Bacillus licheniformis etc . Based on the findings of the study, B . licheniformis is the most potent γ–PGA producing Bacillus sp. which can further be used for the commercial production of γ–PGA. To the best of our knowledge, there is yet no published research from Nepal showing the production of the γ–PGA although microbially produced γ–PGA are the major constituents in some popular foods in particular communities of the country.

show abstract

“…[8][9][10] Recent studies reported that Lysinibacillus fusiformis displayed strong antifungal activity against aflatoxigenic Aspergillus flavus and Aspergillus parasiticus. 11 Lysinibacillus isolate MK212927 is a novel bacterial isolate recovered through a screening program and shown to produce a metabolite with promising antifungal activities against human fungal pathogens. Upon characterization and structure elucidation, it was found to be the allylamine antifungal (terbinafine).…”

Section: Introductionmentioning

confidence: 99%

<p>Optimized Production of the Allylamine Antifungal “Terbinafine” by <em>Lysinibacillus</em> Isolate MK212927 Using Response Surface Methodology</p>

El-Sayed

El-Housseiny

Abdelaziz

et al. 2020

IDR

View full text Add to dashboard Cite

Purpose: We aimed to optimize the factors affecting the production of the allylamine antifungal, terbinafine, by Lysinibacillus isolate MK212927, a natural producer of this broadspectrum fungicidal compound. Methods: We employed a central composite design to optimize the five most important variables influencing the production of terbinafine which were carbon source, nitrogen source, temperature, pH and agitation. Results: The optimum conditions were found to be starch 5 g/L, ammonium chloride 5 g/L, temperature 32°C, agitation 150 rpm and pH 7. The actual response (inhibition zone diameter) was highly comparable to the value predicted by the model, indicating a valid model. Using the standard calibration curve of terbinafine, the optimized conditions resulted in an increase in the antifungal metabolite production (terbinafine) by about 1.6-fold (1814.662 µg/mL compared to 1165.550 µg/mL under standardized conditions). Conclusion: This is the first report, to the best of our knowledge, on optimized production of terbinafine by Lysinibacillus species. Hence, these findings may be useful as baseline data for scaling up the production of terbinafine from a natural microbial source.

show abstract

Screening and Optimization of Newly Isolated Thermotolerant Lysinibacillus fusiformis Strain SK for Protease and Antifungal Activity

Cited by 10 publications

References 40 publications

In vitro antibacterial activities and molecular characterization of bacterial species isolated from farmlands against selected pathogens

In vitro antibacterial activities and molecular characterization of bacterial species isolated from farmlands against selected pathogens

Screening and characterization of potent poly glutamic acid producing Bacillus sp. isolated from Kinema, water and soil samples

<p>Optimized Production of the Allylamine Antifungal “Terbinafine” by <em>Lysinibacillus</em> Isolate MK212927 Using Response Surface Methodology</p>

Contact Info

Product

Resources

About