Screening and Identification of Chitinolytic Actinomycetes and Study on the Inhibitory Activity against Turfgrass Root Rot Disease Fungi

Abstract

Objective: This study aimed to separate and pure chitinase producing actinomycetes with stronger activity from lawn soils of Beijing. Method: Morphology and culture characteristics were observed. physiological and biochemical characteristics were detected. 16SrDNA sequence was analyzed. Result: The highest chitinase activity strain was Bn035, whose relative diameter of transparent circle K2 was 3.42 at which the chitinase activity of fermentation broth reached a maximum value of 1.23 U/mL in the 6th day. Crude enzyme solution and bacteria cake of strain Bn035 generated stronger bacterial resistance to all root rot fungi which indicated that the strain had a strong broad-spectrum resistance to root rot fungi. Conclusion: The strain was identified as Streptomyces scabrisporus. It will be a potential biological control function.

Genome mining of Streptomyces scabrisporus NF3 reveals symbiotic features including genes related to plant interactions

“…There is a high diversity of amino acid posttranslational modification genes, which means that they can produce extremely highly modified peptides, including D-amino acids, methylated amino acids and dehydrated amino acids and dozens of modified residues. The capacity to grow without needing external amino acids can be an advantage in poor nutrient environments and implies that the SS NF3 strain is likely to, at least in some segments of its life cycle, grow in soil communities; growth of another SS strain on various nitrogen sources was reported for peptone, yeast extract, (NH4)2SO4, NH4NO3 and L-asparagine[ 35 ].…”

“…Endophytic Streptomyces spp. are documented to promote plant growth[ 25 , 30 ], enable nutrient procurement[ 31 ], phytohormone synthesis[ 32 – 34 ], antibiosis and local competition against pathogens[ 31 , 35 ], and induction of systemic defense responses[ 24 , 29 , 36 ].…”

“…While few strains of the species have been isolated and characterized in the past (mostly from soils) [ 35 , 41 – 43 ], only one strain of S . scabrisporus was sequenced and published so far: DSM 41855, isolated from soil from Japan.…”

Actinomycetes from the Soil of Chilli Plantation in Yogyakarta Showing an Antagonism to Fusarium oxysporum FU3

“…All assays were done in triplicate. The inhibitory percentage was calculated using the following formula (2): (Wang et al 2015)…”

“…Quorum sensing occurs naturally in biofilm formation, virulence, bioluminescence, symbiosis, conjugative plasmid transfer, competence, motility, sporulation, peptide synthesis and antimicrobials (Kavitha et al, 2014). Wang et al (2015) compared the inhibition of actinomycetes (Bn035) against F. oxysporum in liquid and solid media. In the study, the author used 0.2 mL crude chitinase enzyme produced by Bn035.…”