Microbial chitinases and their relevance in various industries

Thakur, Deepali; Chauhan, Anjali; Jhilta, Prakriti; Kaushal, Rajesh; Dipta, Bhawna

doi:10.1007/s12223-022-00999-w

Cited by 4 publications

(1 citation statement)

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“…Chitin, a highly prevalent natural amino polysaccharide, is generated annually in quantities nearly equal to cellulose (Makino et al, 2006). It is mainly found in the cell walls of fungi, nematodes, mollusk shells, and exoskeletons of arthropods but is absent in vertebrates (Thakur et al, 2023). In insects, chitin is a major component of the exoskeleton and peritrophic matrix (PM) (Zhu et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Alternative splicing of chitin deacetylase 2 regulates chitin and fatty acid metabolism in Asian citrus psyllid, Diaphorina citri

Zhang,

Xia,

Wang

et al. 2023

Arch Insect Biochem Physiol

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Chitin plays an important role in the development and molting of insects. The key genes involved in chitin metabolism were considered promising targets for pest control. In this study, two splice variants of chitin deacetylase 2 (CDA2) from Diaphorina citri were identified, including DcCDA2a and DcCDA2b. Bioinformatics analysis revealed that DcCDA2a and DcCDA2b encoded 550 and 544 amino acid residues with a signal peptide, respectively. Spatio‐temporal expression patterns analysis showed that DcCDA2a and DcCDA2b were highly expressed in D. citri wing and nymph stages. Moreover, DcCDA2a and DcCDA2b expression levels were induced by 20‐hydroxyecdysone (20E). Silencing DcCDA2a by RNA interference (RNAi) significantly disrupted the D. citri molting and increased D. citri mortality and malformation rate, whereas inhibition of DcCDA2b resulted in a semimolting phenotype. Furthermore, silencing DcCDA2a and DcCDA2b significantly suppressed D. citri chitin and fatty acid metabolism. Our results indicated that DcCDA2 might play crucial roles in regulating D. citri chitin and fatty acid metabolism, and it could be used as a potential target for controlling D. citri.

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Section: Introductionmentioning

confidence: 99%