Screening and Identification of Amylase Producing Bacteria from Marakkanam Saltpan Environment, Tamil Nadu, India.

Shanmugasundaram, S.; Annadurai, Eswar; Mayavu, P.; Surya, Maryati; Anbarasu, R.

doi:10.15272/ajbps.v5i48.732

Cited by 7 publications

(2 citation statements)

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“…Congo Red Agar (CRA) Cellulose Gupta et al [11] Xylan agar medium Xylanase Nagar et al [12] Starch agar medium Amylase Shanmugasundaram et al [13] Skim Milk Agar (SMA) Protease Dutta et al [14] Phenotypic bacteria identification Phenotypic bacteria identification is an extensive phenotypic investigation which includes colony morphology and Gram staining. Colony morphology for each bacteria strain was observed under stereomicroscope (Leica) and colony characteristics were described based on Bergey's manual of Systematic Bacteriology [15].…”

Section: Selective Medium Substrate Referencementioning

confidence: 99%

Identification and characterization of potential compost degrading bacteria from agro-waste

Khalif¹,

Nordin²,

Alias

2021

Mal. J. Fund. Appl. Sci.

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Agricultural industry plays a significant role in the global economic growth. It is estimated more than 15% of total waste in Asia are contributed by agro waste. Bacteria is known as one of the useful organisms actively found to surround the waste industry. They are considered as chemical decomposers and act as driving agent of composting that changes the chemistry of organic waste to simple compounds. Thus, this study was conducted to isolate and identify potential compostdegrading bacteria from agriculture waste at several sampling areas in Besut district, Terengganu, Malaysia. A total of 49 bacteria strains were isolated using Tryptic Soya Agar (TSA) from seven groups of raw agro wastes (paddy husk, paddy straw, paddy soil, rock melon waste, rock melon soil, corn waste, and corn soil). Primary screening for potential enzyme production was carried out using selective media containing different substrates (sucrose, xylan, starch, skim milk, and pectin). Only 13 bacterial strains were found positive for protease, nine bacteria strains positive for xylanase, and three bacteria strains were found positive for amylase. Identification of bacteria strains were performed using phenotypic, biochemical tests, and genotypic approaches by 16S rRNA gene sequence. Based on NCBI BLAST analysis, we have identified several bacteria strains: Bacillus cereus (strain B), Alcaligenes faecalis (strain C), Micrococcus sp. (strain D), Pseudomonas stutzeri (strain E), Enterobacter cloacae (strain G), and Serratia marcescens (strain J). Strain F and strain H were identified under distinct family of Enterobacteriaceae, while strain I was identified from Pseudomonadeles order which might represent a new type of proteobacteria strain. These potential waste degrading bacteria could be further analyzed and studies for their true potential in many areas including agriculture and industrial waste management as an approach to reduce waste accumulation in eco-friendly way.

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Section: Selective Medium Substrate Referencementioning

confidence: 99%

Identification and characterization of potential compost degrading bacteria from agro-waste

Khalif¹,

Nordin²,

Alias

2021

Mal. J. Fund. Appl. Sci.

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“…Utilization of highly specific enzymes for various sectors and applications are gaining a momentum because of their ability to replace harsh organic/inorganic chemicals currently used in all industries (Dhiman et al, 2008). The source of enzymes is animal, plant and microorganisms; however, the industrial applications of commercial enzymes, microorganisms are the foremost vital source of assorted enzymes because they are inexpensive to produce and their enzyme contents are more expected, convenient to handle and reliable (Shanmugasundaram et al, 2015). Enzymes are mainly performed in the conversion of macromolecules to body energy and new materials, also for growth, repair and cell maintenance.…”

Section: Introductionmentioning

confidence: 99%

Production and Purification of Amylase from Bacillus subtilis Isolated from Soil

Kalyani¹,

Rajesh²

2018

IJEMR

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In spite of progress in biotechnology and enzymology, the enzymes have been industrialized in recent years for the mounting up the product development in various arena. The ultimate goal of this study comprises the production and purification the amylase enzyme from the bacterial strain. A powerful amylase producer, Bacillus subtilis ISOLATE-4 was isolated, screened and identified from the soil sample. In order to produce extracellular amylase, various physico-chemical parameters were optimized. During optimization, the maximal production of amylase by the isolate at 48 hrs of incubation in 100 rpm was found to be 6.93U/ml, 5.94U/ml, 6.0U/ml at 45ºC, pH 6 with 1% substrate concentration respectively. Ammonium sulphate fractionation was done for rapid precipitation of the amylase at a concentration of 60% and exposed to dialysis showed the 25% purification fold of an enzyme. The dialyzed product was further subjected to DEAE-Cellulose column chromatography resulted in an increase up to 75% purification fold than crude enzyme. The amylase enzyme might be suitable for the liquefaction of starch, detergent, textile and several additional industrial applications.

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Morphological Characterization of Hydrogels

Rahman¹,

Islam²,

Islam³

et al. 2018

Polymers and Polymeric Composites: A Reference Series

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Screening and Identification of Amylase Producing Bacteria from Marakkanam Saltpan Environment, Tamil Nadu, India.

Cited by 7 publications

References 0 publications

Identification and characterization of potential compost degrading bacteria from agro-waste

Identification and characterization of potential compost degrading bacteria from agro-waste

Production and Purification of Amylase from Bacillus subtilis Isolated from Soil

Morphological Characterization of Hydrogels

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