It is concluded that the ability of bacteriocin in inhibiting a wide-range of pathogenic bacteria is of potential interest for food safety and may have future applications in food preservative.

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A study was carried out multiple antibiotic resistance of total heterotrophic bacteria isolated from sediment samples in mangrove environment of Muthupettai, Southeast coast of India was during the period of April 2006-March 07. Six stations at different area of the mangrove sites were selected for sampling. The results of the present study revealed that resistance of heterotrophic bacteria strains (six hundred and eighty) were isolated and identified from mangrove environment. The levels of resistance of bacteria to various antibiotics differed considerably. Among these 6 species (22 strains) multiple antibiotic resistance bacteria were identified from all isolates such as Escherichia coli (6 strains), Vibrio parahaemolyticus (5 strains), Vibrio vulnificus (3 strains), Pseudomonas fluotescens (4 strains), Pseudomonas cepacia (2 strains) and Proteus v ulgaris (2 strains). The all strains were also able to resistance concentration of antibiotics up to 150 µg mLG. The isolated strains were screened 1 for plasmid DNA by agarose gel electrophoresis and tested for susceptibility to 10 antibiotics by the agar dilution method. Twenty two strains belonging to 6 species have been found to Muthupettai mangroves 1-5 plasmids, with sizes ranging from 8-137 kb.

The effect of amylase on growth performance, survival rate and proximate composition of Perinereis cultrifera was investigated. The amylase produced by Bacillus sp. was added to polychaete basal diets at three concentrations such as 50, 100 and 150 mg kg )1 in experiments I, II and III, respectively. After 90 days, polychaete receiving the diets supplemented with enzymes at 150 mg kg )1 showed significantly better growth performance and survival rate than those fed the basal diet (Control). Among these experimental animals, the maximum protein, lipid, carbohydrate and ash contents were recorded in experiment III, whereas moisture content was maximum in control and minimum in experiment III. The essential fatty acids such as palmitic acid (C16:0), margaric acid (C17:0), stearic acid (C18:0) and arachidonic acid (C20:4x-6) occurred at higher concentration in experiment group than in control. Thus, the amylase produced by Bacillus sp. significantly influenced growth, survival rate and proximate composition of P. cultrifera which has importance in aquaculture industry. KEY WORDS