Screening and identification of a Bacillus amyloliquefaciens strain for aqueous enzymatic extraction of medium-chain triglycerides

Zeng, Cheng; Zhao, Rongbin; Wen, Xiaobo; Yu, Ping; Zeng, Zheling; Deng, Qiang; Gong, Deming

doi:10.1016/j.foodcont.2017.02.031

Cited by 6 publications

(3 citation statements)

References 18 publications

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“…CCSK samples were found to have high oil contents, with a mean value of 54.59 g/100 g, among which Chongqing was the highest (62.08 g/100 g), followed by Anhui (61.79 g/100 g) and Guizhou (57.89 g/100 g), and Hunan was the lowest (46.29 g/100 g) ( Table 3 ). The oil contents in CCSKs were consistent with the previous reports [ 3 , 21 ]. As a natural medium-chain triglyceride (MCFA > 95%), the oil showed great potential in preventing obesity, inflammation, cardiovascular disease and diabetes [ 9 , 10 , 22 ].…”

Section: Resultssupporting

confidence: 92%

Geographic Pattern of Variations in Chemical Composition and Nutritional Value of Cinnamomum camphora Seed Kernels from China

Yan

Gong

Zeng

et al. 2023

Foods

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Cinnamomum camphora (camphor tree) is an important non-conventional edible plant species found in East Asia. Here, a detailed characterization for the chemical composition and nutritional value of C. camphora seed kernels (CCSKs) collected from different regions in China is provided. The results showed that there were significant differences among the CCSK samples in weights (1000 fruits, 1000 seeds and 1000 kernels), proximate composition, minerals, phenolics, flavonoids and amino acid contents. The highest contents of oil (62.08%) and protein (22.17%) were found in the CCSK samples collected from Chongqing and Shanghai, respectively. The highest content of mineral in the CCSK samples was K (4345.05–7186.89 mg/kg), followed by P (2735.86–5385.36 mg/kg), Ca (1412.27–3327.37 mg/kg) and Mg (2028.65–3147.32 mg/kg). The CCSK sample collected from Guizhou had the highest levels of total phenolic and flavonoid contents (TPC and TFC), while that from Chongqing had the lowest levels. In addition, the most abundant fatty acid in the CCSK samples was capric acid (57.37–60.18%), followed by lauric acid (35.23–38.29%). Similarities in the fatty acid composition among the CCSK samples were found. The CCSK sample collected from Guizhou had the highest percentage (36.20%) of essential amino acids to total amino acids, and Chongqing had the lowest value (28.84%). These results indicated that CCSK may be developed as an excellent source of plant-based medium-chain oil, protein, dietary fiber, minerals, phytochemicals and essential amino acids.

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Section: Resultssupporting

confidence: 92%

Geographic Pattern of Variations in Chemical Composition and Nutritional Value of Cinnamomum camphora Seed Kernels from China

Yan

Gong

Zeng

et al. 2023

Foods

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“…The organism was B. amyloliquefaciens NCU116 identified by our laboratory (Zeng et al 2017 ). Corn flour, bran and soybean meal were purchased from Nanchang Jingke Co. (Nanchang, China).…”

Section: Methodsmentioning

confidence: 99%

“…NPST-AK15 (Ibrahim et al 2015 ), Bacillus subtilis (Pant et al 2015 ) and Bacillus licheniformis RP1 (Haddar et al 2011 ). On the other hand, the lipase activity of NCU116 was very low (Zeng et al 2017 ), which makes it a suitable strain for CCSKO extraction by AEM. Although the proteinase secreted by B. amyloliquefaciens NCU116 exhibited promising properties, and the proteinase production was increased to 6984.3 U/ml through optimizing cultivation conditions, the yield of proteinase by NCU116 was unsatisfactory for CCSKO extraction by AEM.…”

Section: Introductionmentioning

confidence: 99%

Mutagenesis and characterization of a Bacillus amyloliquefaciens strain for Cinnamomum camphora seed kernel oil extraction by aqueous enzymatic method

Zeng

Zhao

et al. 2017

AMB Expr

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The purpose of the present study was to increase the proteinase activity of the strain NCU116 by combining ultraviolet irradiation and N-methyl-N′-nitro-N-nitroso guanidine treatment, in order to enhance the efficiency of Cinnamomum camphora seed kernel oil (CCSKO) extraction by aqueous enzymatic method (AEM). The mutated strain, designated as NCU116-1, was screened out by the ratio of hydrolytic zone diameter to colony diameter on skim milk plate. The proteinase activity (9116.1 U/ml) of NCU116-1 was increased by 31.9% compared with the parental strain. The extracellular enzymes produced by NCU116-1 included proteinase, pectase, glucoamylase, cellulase and amylase. The proteinase had the maximum activity at 50 °C. Its optimum temperature and pH value were approximately 45 °C and 8.0 respectively. Mn2+ was an activator of neutral proteinase. The glucoamylase had the maximum activity at 35 °C, and was activated by Cu2+, Fe3+ and Mn2+. Its optimum temperatures and pH value were 35 °C and 8.0 respectively. The pectinase had the maximum activity at 40 °C, and was activated by Ca2+ and Mn2+. Its optimum temperatures and pH value were 35–40 °C and 6.0 respectively. The optimum conditions of CCSKO extraction by AEM were also investigated. The results suggested that the best amount of enzyme solution and enzymolysis time were 20% (v/v) and 4 h, respectively. The oil extraction rate was 95.2% under these conditions. Thus, a suitable mutated strain was selected for CCSKO extraction by AEM and the optimum extraction conditions were determined.

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Regulation mechanism of magnetic field on pectinase and its preliminary application in postharvest sapodilla (Manilkara zapota)

et al. 2023

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Screening and identification of a Bacillus amyloliquefaciens strain for aqueous enzymatic extraction of medium-chain triglycerides

Cited by 6 publications

References 18 publications

Geographic Pattern of Variations in Chemical Composition and Nutritional Value of Cinnamomum camphora Seed Kernels from China

Geographic Pattern of Variations in Chemical Composition and Nutritional Value of Cinnamomum camphora Seed Kernels from China

Mutagenesis and characterization of a Bacillus amyloliquefaciens strain for Cinnamomum camphora seed kernel oil extraction by aqueous enzymatic method

Regulation mechanism of magnetic field on pectinase and its preliminary application in postharvest sapodilla (Manilkara zapota)

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