Regulation mechanism of magnetic field on pectinase and its preliminary application in postharvest sapodilla (Manilkara zapota)

Regulation effect of magnetic field combined with low temperature storage on postharvest quality and cell wall pectic-polysaccharide degradation of Clausena lansium (Lour.) Skeels

“…5 , there seemed to be no obvious pattern in terms of magnetic field effect on monosaccharide compositions of pectic-polysaccharides except fluctuation of contents in certain monosaccharides. In our previous study, it was proved that magnetic field (3 mT, 30 min) could inhibit activity of pectinase as well as alter its secondary structure and then delayed fruit softening of postharvest sapodilla, which was also prefect explanation of present results ( Sun et al, 2023 ).…”

“…The intuitive and measurable changes were shifting enzymic structure with activity alteration for these enzymes mentioned above. And our previously published research also reported that magnetic field treatment could change the polarity of aromatic amino acid residues and cause the modification of functional groups as well as secondary structure in pectinase, thus altering pectinase activity ( Sun et al, 2023 ). Therefore, altering enzymic activity is one of directly reasons to degrade cell wall pectic-polysaccharide and disassemble fruit′s cell wall construction, which led to the delay of fruit softening.…”

Production, Kinetic/Thermodynamic Study, and Evaluation of the Influence of Static Magnetic Field on Kinetic Parameters of β-Fructofuranosidase from Aspergillus tamarii Kita UCP 1279 Produced by Solid-State Fermentation

“…Similar qualitative behavior for K m was observed by Liu et al [ 14 ] when the immobilized α-amylase kinetic parameters in the presence of 0.15 T were evaluated. However, other authors, such as Albuquerque et al [ 17 ], did not observe significant differences in K m and V max for fibrinolytic protease from Mucor subtilissimus and a negative impact of SMF was evidenced by increase in K m and decrease in V max , as reported by Sun et al [ 31 ] for a vegetable pectinase. Among the different mechanisms involved in modifications of biocatalyst activity by exposure to SMF, the radical pair recombination has been the preferred explanation, though it requires conditions at moderate magnetic fields to be effective [ 17 , 32 ], which is the case of the present experiment.…”