Screening and functional prediction of differentially expressed circular RNAs in human glioma of different grades

Abstract

Circular RNAs (circRNAs) have a critical regulatory function in human glioma. However, novel circRNAs related to different pathological grades of glioma and their crucial potential function are worth screening and prediction. CircRNA expression profiling was performed for 6 paired high- and low-grade glioma tissues and 5 adjacent normal brain tissues through next-generation sequencing. Quantitative real-time PCR (qRT-PCR) was conducted to validate circRNA expression. Bioinformatics analysis was performed, and circRNA-miRNA-mRNA networks were constructed. The expression and survival data of miRNAs and target genes were examined by GEPIA, Chinese Glioma Genome Atlas (CGGA), ONCOMINE, and cBioPortal databases. The RNA binding proteins (RBPs), open reading frames (ORFs) and N6-methyladenosine (m 6 A) modifications of the identified circRNAs were also predicted. Through multilevel research screening, 4 circRNAs (hsa_circ_0000915, hsa_circ_0127664, hsa_circ_0008362, and hsa_circ_0001467) were associated with glioma of different pathological grades and could be preferred candidates for subsequent functional analysis. Therefore, circRNAs are associated with the different pathological grades of glioma and reveal their potential critical regulatory function. CircRNAs might provide vital molecular biomarkers and potential therapeutic targets for glioma.

circKCNN2 suppresses the recurrence of hepatocellular carcinoma at least partially via regulating miR‐520c‐3p/methyl‐DNA‐binding domain protein 2 axis

“…Furthermore, the low level of circKCNN2 was significantly associated with unfavorable OS and RFS of HCC. circKCNN2 was previously reported to be associated with the development of glioma 22 . These data, together with our results, indicate that circKCNN2 may function as a tumor suppressor in multiple cancer types.…”

“…Fifteen potentially functional circRNAs that had at least one significantly enriched gene set were selected from the 53 circRNA candidates (Figure 1A, right). Among the 15 circRNAs, circKCNN2 (also known as hsa_circ_0127664) was previously reported to be significantly downregulated in brain tissue of patients with progressive glioma, suggesting it may be a tumor suppressor 22 . Therefore, circKCNN2 was selected for further investigation as a potential suppressor for HCC recurrence.…”

Identification and characterization of N6‐methyladenosine modification of circRNAs in glioblastoma

“…Many studies have suggested that epigenetic modification plays a crucial role in the pathogenesis of GBM. 20,21 Recently, N6methyladenosine modification has attracted extensive attention, but the specific mechanism of this novel RNA modification in the occurrence and development of GBM has not been fully studied, especially in circRNA. 11 We used RNA N6-methyladenosine sequencing to explore the state of N6-methyladenosine-circRNA modifications in GBM tissues.…”

Molecular mechanism of m6A methylation of circDCL1 mediated by RNA methyltransferase METTL3 in the malignant proliferation of glioma cells

“…Glioma presents a challenging clinical situation that causes notable mortality and morbidity [1]. circRNAs make a signi cant contribution to the regulation of cell cycle, angiogenesis, metastasis, and drug resistance of glioma cells [33]. This study elucidated that METTL3-mediated circDCL1 promoted CTNNBIP1 transcription by sponging miR-671-5p, thus repressing the malignant proliferation of glioma cells (Fig.…”