Screening and Evaluation of Biosurfactant-Producing Strains Isolated from Oilfield Wastewater

Liu, Jianghong; Yi-tong, Chen; Xu, Ran; Yun-peng, Jia

doi:10.1007/s12088-013-0379-y

Cited by 45 publications

(24 citation statements)

References 16 publications

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“…Though hydrocarbon contaminated sites are the most promising source for the isolation of biosurfactant producers, there are few reports which elucidated the isolation of biosurfactant producers from diverse environments [20]. It has been reported that microorganisms from marine origin have shown to produce novel biosurfactants with excellent bioactivity [21].…”

Section: Introductionmentioning

confidence: 99%

Antibiofilm Activity of Biosurfactant Producing Coral Associated Bacteria Isolated from Gulf of Mannar

Padmavathi

Pandian

2014

Indian J Microbiol

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Coral Associated Bacteria (CAB) (N = 22) isolated from the mucus of the coral Acropora digitifera were screened for biosurfactants using classical screening methods; hemolysis test, lipase production, oil displacement, drop collapse test and emulsifying activity. Six CAB (U7, U9, U10, U13, U14, and U16) were found to produce biosurfactants and were identified by 16S ribosomal RNA gene sequencing as Providencia rettgeri, Psychrobacter sp., Bacillus flexus, Bacillus anthracis, Psychrobacter sp., and Bacillus pumilus respectively. Their cell surface hydrophobicity was determined by Microbial adhesion to hydrocarbon assay and the biosurfactants produced were extracted and characterized by Fourier Transform Infrared spectroscopy. Since the biosurfactants are known for their surface modifying capabilities, antibiofilm activity of positive isolates was evaluated against biofilm forming Pseudomonas aeruginosa ATCC10145. Stability of the active principle exhibiting antibiofilm activity was tested through various temperature treatments ranging from 60 to 100°C and Proteinase K treatment. CAB isolates U7 and U9 exhibited stable antibiofilm activity even after exposure to higher temperatures which is promising for the development of novel antifouling agents for diverse industrial applications. Further, this is the first report on biosurfactant production by a coral symbiont.

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Section: Introductionmentioning

confidence: 99%

Antibiofilm Activity of Biosurfactant Producing Coral Associated Bacteria Isolated from Gulf of Mannar

Padmavathi

Pandian

2014

Indian J Microbiol

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“…The oil spreading method is another effective technique to detect the presence of biosurfactants. In this method, if the biosurfactant is present in the supernatant, the oil is displaced and a clearing zone is formed (Liu et al 2013). The diameter of this clearing zone on the oil surface correlates with surfactant activity, also called oil displacement activity.…”

Section: Discussionmentioning

confidence: 99%

Biosurfactant production by Trichoderma sp. MK116452 and its possible application in oil recovery

Matussin¹,

Shivanand

Lim

2020

Preprint

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Abstract Surfactants are amphiphatic compounds containing both hydrophilic and hydrophobic groups, which are capable of lowering surface or interfacial tension. Considering the advantages of using biosurfactants produced by microorganisms, the aim of this study was to develop and characterise a biosurfactant produced by Trichoderma sp. MK116452 isolated from Seri Cermin filling station, Brunei Darussalam, and to examine its potential application in microbial enhanced oil recovery (MEOR). The microorganism was cultured in a minimal salt medium containing 1% crude oil as a carbon source and a cell-free culture broth was used to screen its efficiency in producing biosurfactants. Characterisation of the biosurfactant showed the presence of glycosides and fatty acids, suggesting its glycolipidic nature. The isolated biosurfactant showed no toxicity to the micro-crustacean Artemia salina or to red bean ( Vigna angularis ). The extracted biosurfactant was effective at recovering up to 60% of crude oil from sand using the sand pack column method, which is similar to the recovery rate for a chemical surfactant (SDS) (65%). These findings highlight the potential use of Trichoderma sp. MK116452 biosurfactant in the oil industry.

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“…After 7 days, crude oil was dehydrated. Then the viscosity of crude oil was measured by a Rotating Viscometer (NDS-8S, China) that was set at 50 °C and 12 s -1 shear rate 7 .…”

Section: Methodsmentioning

confidence: 99%

Microbial Paraffin-Removal Technology Using Paraffin-Degrading and Biosurfactant-Producing Strain

Liu¹,

Chen²,

Xu³

et al. 2014

Asian J. Chem.

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Paraffin deposition on the walls of wells and collection pipelines, which causes problems for the production and transportation of crude oil, is a common phenomenon 1 .Several methods have been used to overcome the problem of paraffin deposition, including mechanical, thermal, chemical and microbial methods 2,3 . For mechanical treatment, which is not harm for the strata, but the stability is not strong, also is time-consuming. Thermal treatment, which is improved on paraffin removal, results in the formation damage by concentrating heavier ends of the oil and paraffin, which can be mobilized by the heat available through hot oiling, The chemical treatment, which needs less investment in equipment, is costly and highly toxic 4,5 . Microbial treatment has many outstanding advantages: simple construction, low operating costs, the role of a long cycle does not affect the quality of the oil strata without any damage.In this work, paraffin-degrading strain was obtained by separation and purification experiment from paraffinic oil production wells in Daqing Oilfield. The paraffin-degrading strain was named as P1 and biosurfactant-producing strain was named as S1. As an indicator of the degradation of paraffin, strain P1 and strain S1 were added in different proportions and then the optimum proportion was obtained. Research of mixed The paraffin-degrading strain, isolated from waxy oil production wells in Daqing oilfield, were obtained by separation and purification experiment. The paraffin-degrading strain P1 was identified through 16S rDNA sequence analysis, which exhibited the highest similarities to Bacillus anthracis strain XFB-BN. The biosurfactant-producing strain was obtained by laboratory saved. After paraffin-degrading and biosurfactant-producing strain treatment, the paraffin degradation rate could reach to 68.2 %, the paraffin prevention rate could reach to 80.7 %, the reduction rate of crude oil viscosity was 62.4 %. By analyzing the data, the paraffin-degrading and biosurfactant-producing strain had better effect on microbial prevention of wax deposition.

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Screening and Evaluation of Biosurfactant-Producing Strains Isolated from Oilfield Wastewater

Cited by 45 publications

References 16 publications

Antibiofilm Activity of Biosurfactant Producing Coral Associated Bacteria Isolated from Gulf of Mannar

Antibiofilm Activity of Biosurfactant Producing Coral Associated Bacteria Isolated from Gulf of Mannar

Biosurfactant production by Trichoderma sp. MK116452 and its possible application in oil recovery

Microbial Paraffin-Removal Technology Using Paraffin-Degrading and Biosurfactant-Producing Strain

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