Screening and evaluation of active compounds in polyphenol mixtures by HPLC coupled with chemical methodology and its application

Zhao, Yue; Wang, Ying; Jiang, Zi‐Tao; Li, Rong

doi:10.1016/j.foodchem.2017.01.085

Cited by 26 publications

(24 citation statements)

References 33 publications

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“…OH radical scavenging assay was performed following the method of previous literature (Zhao et al, ) with a slight modification. An aliquot of 0.5 ml of FeSO 4 (0.2 mol/L) solution and 1.0 ml of REE solution (1.0 mg/ml) were added into a 10 ml volumetric tube and mixed well.…”

Section: Methodsmentioning

confidence: 99%

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Antioxidant activity evaluation of rosemary ethanol extract and their cellular antioxidant activity toward HeLa cells

Nie

Wang

et al. 2019

J Food Biochem

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Rosemary ethanol extract (REE) from Rosmarinus officinalis was identified by LC‐ESI‐MS/MS and 12 compounds were found. Among them, rosmarinic acid (389.78 μg/mg in REE), luteolin‐3ʹ‐O‐glucuronide (325.58 μg/mg), luteolin‐5‐O‐glucuronide (120.92 μg/mg), and geniposide (120.83 μg/mg) are the major components. The antioxidant activity evaluation of REE by off‐line HPLC methods indicated that among the 12 compounds, rosmarinic acid had the strongest scavenging activities in both DPPH· and ·OH. The cytotoxicity experiment showed that REE with the concentration ranges from 1 to 100 µg/ml did not significantly affect the cell viability of HeLa, while inhibitory rate reduced to 62.3% when the concentration was increased to 1,000 µg/ml. The results of intracellular antioxidation assay showed that the ability of REE in reducing the reactive oxygen species (ROS) in HeLa cells was higher than rosmanol, and lower than rosmarinic acid without cell toxicity. Practical applications Plant polyphenols are essential components of functional foods, due to their antioxidant and enzyme inhibition activities. This paper is the first study about the quantification of antioxidant compounds, antioxidant activity evaluation, and their cellular antioxidant activity of polyphenols extract from R. officinalis toward HeLa cells. We aimed to elucidate the chemical composition and recognition of antioxidant components with DPPH and OH free radicals scavenging activity. In addition, the polyphenols dose‐response correlations with cellular antioxidant activity were also determined. These results indicated that off‐line HPLC method with DPPH and OH free radicals as markers is available for screening antioxidant activity of polyphenols from the mixture.

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Section: Methodsmentioning

confidence: 99%

“…OH radical scavenging assay was performed following the method of previous literature (Zhao et al, 2017) with a slight modification.…”

Section: Hplc-oh Radical Scavenging Assaymentioning

confidence: 99%

Antioxidant activity evaluation of rosemary ethanol extract and their cellular antioxidant activity toward HeLa cells

Nie

Wang

et al. 2019

J Food Biochem

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“…strong antioxidant activity among extracts from the five Moraceae species or hybrids, and 23 phenolic substances were identified via UPLC-QTOF-MS/MS. We next used DPPH/ABTS-guided HPLC assays, a rapid method for screening active antioxidant compounds from complex mixture extracts 36 , to screen effective antioxidants and evaluate the contribution of individual compounds to total antioxidant activity within a complex mixture extract 21 . When an antioxidant compound reacts with a radical such as DPPH• or ABTS• + , the result is a redox reaction; i.e., the molecular structure of the antioxidant changes 37 , with the peak area (PA) becoming significantly smaller in the chromatogram 38 .…”

Section: Screening Of Active Antioxidants By Dpph/abts-guided Hplc Anmentioning

confidence: 99%

Antioxidant evaluation-guided chemical profiling and structure-activity analysis of leaf extracts from five trees in Broussonetia and Morus (Moraceae)

Cao

Yang

Xue

et al. 2020

Sci Rep

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Morus and Broussonetia trees are widely used as food and/or feed. Among 23 phenolics identified from leaves of five Moraceae species using UPLC-QTOF-MS/MS, 15 were screened using DPPH/ABTSguided HPLCs, including seven weak (flavonoids with one hydroxyl on B-ring) and eight strong (four caffeoylquinic acids and four flavonoids, each with a double hydroxyl on B-ring) antioxidants. We then determined the activity and synergistic effects of individual antioxidants and a mixture of the eight strongest antioxidants using DPPH-guided HPLC. Our findings revealed that (1) flavonoid glucuronide may have a more negative effect on antioxidant activity than glucoside, and (2) other compounds in the mixture may exert a negative synergistic effect on antioxidant activity of the four flavonoids with B-ring double hydroxyls but not the four caffeoylquinic acids. In conclusion, the eight phenolics with the strongest antioxidant ability reliably represented the bioactivity of the five extracts examined in this study. Moreover, the Morus alba hybrid had more phenolic biosynthesis machinery than its cross-parent M. alba, whereas the Broussonetia papyrifera hybrid had significantly less phenolic machinery than B. papyrifera. This difference is probably the main reason for livestock preference for the hybrid of B. papyrifera over B. papyrifera in feed.Morus and Broussonetia tree species (family: Moraceae) have high economic value; among other uses, their leaves are widely used as feed to improve meat quality. The genus Broussonetia comprises five species: B. papyrifera, B. kazinoki, B. zeylanica, B. kaempferi, and B. kurzii 1 , some of which are used as folk medicinal plants and/or for making bark paper 2 . The bark, fruits, leaves, and roots of B. papyrifera and B. kazinoki exhibit antioxidant, anti-inflammatory, antinociceptive, and anti-tyrosinase activity 3-6 . In China, B. papyrifera and B. kazinoki leaves, from which several bioactive components such as polyphenols, flavonoids and alkaloids have been isolated 7 , are widely used to feed livestock for meat quality improvement. Methanol extracts from B. papyrifera leaves have greater inhibitory effects on mushroom tyrosinase and 2,2-diphenyl-1-picrylhydrazyl/(DPPH)• scavenging activity 8 , and ethanol extracts from B. papyrifera roots inhibit both acetyl-and butyl-cholinesterases 2 . Papyriflavonol A, isolated from the root bark of B. papyrifera, exhibits strong antimicrobial activity against different pathogenic bacteria and fungi 9 , whereas a 70% ethanol leaf extract of B. kazinoki was reported to dramatically reduce ear and dorsal skin thickness and other clinical symptoms in mice with atopic dermatitis, possibly via the downregulation of immunoglobulin E (IgE) and interleukin-4 (IL-4) plasma levels 5 . Broussonone A extracted from B. kanzinoki stem bark displays noncompetitive inhibitory activity against pancreatic lipase 10 . However, data on the antioxidant activity and bioactive composition of B. kanzinoki remains limited.The genus Morus comprises 10-16 species 11 , of whic...

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“…Polyphenols are plant secondary metabolites that have a wide range of structure‐related bioactivities (Márcio, Morales, & Ferreira, ; Zhao, Ying, Zi, & Rong, ). Studies have indicated that they exhibit anti‐inflammatory effects, inhibit α ‐glucosidase and have antioxidant and antimicrobial effects.…”

Section: Introductionmentioning

confidence: 99%

“…In addition, polyphenols of plant origin are becoming popular choices for food supplements (Gautam et al, ; Ghanemi et al, ). Therefore, researchers in various fields, including pharmaceuticals, food industries, cosmetics and agricultural sectors, are showing increased interest in the study of polyphenols (Gautam et al, ; Márcio et al, ; Zhao et al, ). Humulus japonicus Siebold & Zucc is a perennial medicinal herb from the Cannabaceae family (also called the Hemp family; Čeh, Milica, Iztok, & Veronika, ).…”

Section: Introductionmentioning

confidence: 99%

LC‐MS/MS profiling of polyphenol‐enriched leaf, stem and root extracts of Korean Humulus japonicus Siebold & Zucc and determination of their antioxidant effects

Choi

Desta

Lee

et al. 2018

Biomedical Chromatography

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Polyphenols from ethyl acetate extracts from the leaves, stems and roots of Korean Humulus japonicus were comprehensively profiled using liquid chromatography-electrospray ionization-tandem mass spectrometry. A total of 36 polyphenols were detected, of which 26 were structurally characterized based on their [M - H] peak, tandem mass spectrometry fragmentation pattern, UV-vis absorption and published data. Validation data provided satisfactory results for the evaluated parameters. The determination coefficients were ≥0.9812. The limits of detection and quantification were 0.017-0.573 and 0.056-1.834 mg/L, respectively, indicating good performance limits. The accuracy (expressed as percentage recovery) at 50 and 100 mg/L was 71.4-99.7 and 75.1-105.1%, with precisions (expressed as relative standard deviation) of 1.5-7.3 and 0.8-4.1%, respectively, indicating acceptable accuracy and precision values. The leaves were rich in total polyphenols (3089.9 ± 6.4 mg/kg of fresh sample) followed by the stems (1313.9 ± 6.4 mg/kg of fresh sample) and roots (655.2 ± 2.7 mg/kg of fresh sample). Antioxidant activity, determined by α,α-diphenyl-β-picrylhydrazyl, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) scavenging activity and ferric reducing antioxidant power assay, revealed the lowest EC value for the leaf extracts, indicating a higher scavenging activity in this tissue followed by the roots and stems. Overall, the results indicated that H. japonicus is rich in polyphenols and could be a potential alternative to Humulus lupulus (hop plant) in the brewery industry.

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Screening and evaluation of active compounds in polyphenol mixtures by HPLC coupled with chemical methodology and its application

Cited by 26 publications

References 33 publications

Antioxidant activity evaluation of rosemary ethanol extract and their cellular antioxidant activity toward HeLa cells

Antioxidant activity evaluation of rosemary ethanol extract and their cellular antioxidant activity toward HeLa cells

Antioxidant evaluation-guided chemical profiling and structure-activity analysis of leaf extracts from five trees in Broussonetia and Morus (Moraceae)

LC‐MS/MS profiling of polyphenol‐enriched leaf, stem and root extracts of Korean Humulus japonicus Siebold & Zucc and determination of their antioxidant effects

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