Screening and Estimation of Allelic Differentiation in Indian mustard Using SSR Markers for Background Selection

Singh, Shailja; Singh, V. V.; Ambawat, Supriya; Dubey, Monika; Singh, Dhiraj

doi:10.20546/ijcmas.2017.609.308

Cited by 11 publications

(10 citation statements)

References 27 publications

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“…Here, in this study, PIC values ranged from 0.33 (PSMP 2059) to 0.76 (PSMP 2203) ( Table 1) with an average of 0.55. In some of the previous reports, PIC values varying between 0.02 to 0.97 were reported [31,27,[35][36][37]. An average PIC value of 0.55 observed in the present study is similar to 0.56 and 0.58 reported by other investigators [27,29], respectively.…”

Section: Molecular Characterization and Ssr Analysissupporting

confidence: 89%

“…High PIC values may be observed due to the use of large number of informative markers [40]. Similar reports were observed in several other studies in different crops like pearl millet [12,27,28,29], rice [30,36] and brassica [31].…”

Section: Molecular Characterization and Ssr Analysissupporting

confidence: 87%

“…Such observations could be attributed to diverse world collection of germplasm. Similar results regarding effectiveness of SSR markers in monitoring genetic diversity have also been reported by other investigators [12,15,27,[29][30][31]. Several reports have been observed on estimation of genetic diversity among parental lines of pearl millet on the basis of molecular profiling [14,15,16,11,17,27].…”

Section: Molecular Characterization and Ssr Analysissupporting

confidence: 85%

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Molecular Characterization and Genetic Diversity Analysis of Released Hybrids and Varieties of Pearl Millet [Pennisetum glaucum (L.) R. Br.]

Ambawat¹,

Satyavathi²,

Khandelwal³

et al. 2020

CJAST

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Pearl millet is a climate-resilient crop which is most widely grown in the arid and semi-arid tropics of Asia and Africa over 26 mha. It is a highly nutritious cereal crop and rightly termed as nutricereal. This crop requires low inputs and delivers high cost-effective benefits. Development of high yielding hybrids is the major target of pearl millet researchers globally. The understanding of genetic diversity is very important and must for developing superior hybrids and crop improvement programs. In the present study, we evaluated the diversity among 30 different released hybrids and varieties of pearl millet using 125 Simple Sequence Repeat (SSR) markers. Out of these, 61 polymorphic SSRs were reported giving 191 alleles with an average of 3.13 alleles per primer. Polymorphic Information Content (PIC) varied from 0.33 to 0.76 with an average of 0.55 PIC value. The cluster analysis based on these SSR markers categorized the genotypes into four major clusters viz., I, II, III, IV with similarity coefficient ranging from 0.58 to 0.73. The results depicted that sufficient genetic variability exists among the different hybrids and varieties used in the study which can further prove useful for pearl millet improvement programs. The study also reveals that SSR markers are proficient and may be used efficiently for genetic diversity studies in pearl millet. It is also anticipated that findings of this study may be further used for DNA fingerprinting and varietal identification.

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Section: Molecular Characterization and Ssr Analysissupporting

confidence: 89%

Section: Molecular Characterization and Ssr Analysissupporting

confidence: 87%

Section: Molecular Characterization and Ssr Analysissupporting

confidence: 85%

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Molecular Characterization and Genetic Diversity Analysis of Released Hybrids and Varieties of Pearl Millet [Pennisetum glaucum (L.) R. Br.]

Ambawat¹,

Satyavathi²,

Khandelwal³

et al. 2020

CJAST

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“…Thus, PSMP2066 is the most informative and best marker for characterization followed by PSMP2077, CTM21 and PSMP2072 markers while maker IPES0218 was reported to be the least powerful marker. PIC values ranging from 0.02 to 0.97 were reported by many researchers [21,[23][24][25][26]. An average PIC value of 0.53 observed in the present study is similar to 0.55, 0.56 and 0.58 reported by other investigators [10,21,27], respectively.…”

Section: Molecular Characterization and Ssr Analysissupporting

confidence: 89%

“…An average PIC value of 0.53 observed in the present study is similar to 0.55, 0.56 and 0.58 reported by other investigators [10,21,27], respectively. PIC values can range between 0 and 0.5 because of bi-allelic nature of SNPs while it can go above 0.5 in case of SSRs due to their mutli-allelic nature as reported by other researchers [10,17,23]. Thus, SSR markers are the most preferred and efficient markers due to their ability to detect multiallelic loci, easy to use, co-dominance, higher reproducibility, high polymorphism with vast ability to differentiate the genotypes [28].…”

Section: Molecular Characterization and Ssr Analysismentioning

confidence: 99%

Molecular Characterization of Drought Tolerant Genotypes of Pearl Millet [Pennisetum glaucum (L.) R. Br.] for A1 Zone

Ambawat

Satyavathi

Meena

et al. 2021

IJECC

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Pearl millet is a widely grown, climate resilient rainfed cereal crop cultivated on 29 million ha in the arid and semi-arid tropical regions of Asia and Africa accounting for almost half of global millet production. It is useful for minimizing the adverse effect of climate change, hence facilitating income and food security among farming communities. It has deep root system and exhibit climate-resilient features including adaptation to a wide range of ecological conditions, less irrigational requirements, better growth and productivity in low nutrient input conditions, less dependent on synthetic fertilizers and minimum vulnerability to environmental stresses and thus can survive in harsh climatic conditions, less fertile soil under water scarcity. Breeding of drought tolerant varieties and selecting genotypes for better water use efficiency is important in pearl millet to mitigate the changing climatic scenario. In this study, 24 genotypes of pearl millet which are drought tolerant and specific for A1 zone were characterized using 15 drought specific SSR primers. All the 15 SSRs amplified products of varying sizes ranging between 90-550 bp. A total of 40 alleles were obtained in this study and the number of alleles per locus varied between 2 to 5 with an average of 2.67 alleles. Polymorphic Information Content (PIC) varied from 0.34 to 0.76 with an average of 0.53 PIC value. This study will be useful for developing high yielding, dual purpose cultivars for low rainfall areas i.e. A1 zone and increasing pearl millet productivity.

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Investigation of genetic diversity of different spring rapeseed (Brassica napus L.) genotypes and yield prediction using machine learning models

Norouzi,

Ahangar,

Payghamzadeh

et al. 2024

Genet Resour Crop Evol

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Screening and Estimation of Allelic Differentiation in Indian mustard Using SSR Markers for Background Selection

Cited by 11 publications

References 27 publications

Molecular Characterization and Genetic Diversity Analysis of Released Hybrids and Varieties of Pearl Millet [Pennisetum glaucum (L.) R. Br.]

Molecular Characterization and Genetic Diversity Analysis of Released Hybrids and Varieties of Pearl Millet [Pennisetum glaucum (L.) R. Br.]

Molecular Characterization of Drought Tolerant Genotypes of Pearl Millet [Pennisetum glaucum (L.) R. Br.] for A1 Zone

Investigation of genetic diversity of different spring rapeseed (Brassica napus L.) genotypes and yield prediction using machine learning models

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