Screening and characterization of protease producing actinomycetes from marine saltern

Suthindhiran, K.; Jayasri, M. A.; Dipali, Dipa; Prasar, Apurva

doi:10.1002/jobm.201300563

Cited by 28 publications

(12 citation statements)

References 55 publications

(74 reference statements)

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“…Exploitation of either unexplored or less explored ecosystems for such actinomycetes is highly necessary. Despite Indian marine ecosystem is considered as an important source for the microbial expedition, the studies on the diversity of actinomycetes and bioactive metabolite’s production from Indian peninsula are scanty and need to be explored [10].…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of bioactive metabolites producing marine Streptomyces parvulus strain sankarensis-A10

Shaik

Sankar

Iswarya

et al. 2017

Journal of Genetic Engineering and Biotechnology

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The significance and frequency of marine microorganisms as producers of bioactive metabolites-a natural source of drug discovery had varied significantly during the last decades, making marine ecosystem a huge treasure trove of novel isolates and novel compounds. Among the twelve actinomycetes isolated from marine sediment sample (Lat. 17°41′962″N, Long. 83°19′633″E), amylase, protease, lipase and cellulase activities were exhibited by 8,7,4,3 isolates respectively. Five isolates exhibited l-asparaginase activity, while 5, 6, 2 isolates exhibited antibacterial, antifungal and antimicrobial activities respectively. One isolate VMS-A10 efficiently producing alpha-amylase (25.53 ± 0.50 U/mL), protease (19.26 ± 0.25 U/mL), lipase (36.25 ± 0.10 U/mL), cellulase (14.43 ± 0.513 U/mL), l-asparaginase (0.125 ± 0.004 U/mL), antimicrobial metabolites against B. subtilis (503.33 ± 5.77 U/mL), S. aureus (536.66 ± 5.77 U/mL), E. coli (533.33 ± 5.77 U/mL), P. aeruginosa (500.00 ± 10.0 U/mL), MRSA (538.33 ± 5.77 U/mL), C. albicans (353.33 ± 11.54 U/mL) and A. niger (443.33 ± 15.27 U/mL) was selected, identified on the basis of morphological, cultural, physiological, and biochemical properties together with 16S rDNA sequence, designated as Streptomyces parvulus strain sankarensis-A10 and sequencing product (1490 bp) was deposited in the GenBank database under accession number KT906299, Culture Deposit No: NCIM-5601. Isolation and characterization of each potential actinobacteria having immense industrial and therapeutic value on an unprecedented scale from marine sediments of Visakhapatnam coast will have a burgeoning effect.

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Section: Introductionmentioning

confidence: 99%

Isolation and characterization of bioactive metabolites producing marine Streptomyces parvulus strain sankarensis-A10

Shaik

Sankar

Iswarya

et al. 2017

Journal of Genetic Engineering and Biotechnology

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“…However, the Actinomycetes diversity of this extreme environment is not adequately studied (Suthindhiran et al . ). Research on the diversity of Actinomycetes from the southern coast of India is still understudied and indefinite.…”

Section: Resultsmentioning

confidence: 97%

Marine Actinomycetes as potential source for histone deacetylase inhibitors and epigenetic modulation

Varghese

Jayasri

Suthindhiran

2015

Lett Appl Microbiol

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Histone deacetylases (HDAC) are epigenetic enzymes that regulate the deacetylation in lysine group on a histone, and thus regulate the gene expression. The HDAC inhibitors are reported to promote apoptosis on tumour cells, thus become clinically important drug target. Several studies have addressed the identification of putative HDAC inhibitors as therapeutic agents for cancer and until now those cleared phase III human trials are very limited. This study attempts to investigate the chemical diversity found in marine Actinomycetes towards negative HDAC modulation, which could be used individually or in combination as anti-cancerous and other therapeutic measure.

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“…The protein present in the supernatant was precipitated using acetone at different concentrations (50%, 75%, and 100%). The precipitated protein was further characterized as described earlier (Suthindhiran et al 2014).…”

Section: Enzyme Production and Puri Cationmentioning

confidence: 99%

Isolation and Bioprocess Optimization of Halophilic and Alkaline Protease From Marine Streptomyces and Its Use As Contact Lens Cleaner

Sarkar

Suthindhiran

2020

Preprint

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Abstract In the present study, an alkaline protease was isolated from marine Streptomyces sp. GS – 3. The strain was isolated from the backwaters of Puthuvypeen, Kerala, and identified as Streptomyces sp. by polyphasic taxonomy. The media for protease production was optimized by response surface methodology (RSM) using the Box-Behnken model. The maximum yield (357 U/ml) was observed using wheat bran as substrate (5.5%), pH 5.5, and the incubation period of 9 days at 28 ᴼC. The optimum temperature and pH conditions for the maximum enzyme activity were 45 °C, and 9 respectively. The Km and Vmax values of the enzyme were determined as 5.88%, and 38.46 µmol l− 1 min− 1 mg− 1, respectively, using 1% casein as substrate. The enzyme was isolated by solvent extraction with acetone, and the crude enzyme was characterized by Sodium Dodecyl Sulphate Poly-Acrylamide Gel Electrophoresis (SDS-PAGE) and Circular Dichroism (CD) studies. The enzyme sustained at high temperature (50 °C for 6 h), and alkaline pH (10) conditions, and was active in the presence of metal ions, and organic solvents. The purified enzyme was inhibited by phenylmethylsulphonyl fluoride (PMSF) suggesting it belong to serine protease. Further to exploit its application, contact lenses were incubated with the enzyme solution, and the protein debris on it was found to be scrubbed at an optimum time of 60 minutes. Therefore, it increases the transmittance of the contact lens indicating its use as a potential contact lens cleansing agent.

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Screening and characterization of protease producing actinomycetes from marine saltern

Cited by 28 publications

References 55 publications

Isolation and characterization of bioactive metabolites producing marine Streptomyces parvulus strain sankarensis-A10

Isolation and characterization of bioactive metabolites producing marine Streptomyces parvulus strain sankarensis-A10

Marine Actinomycetes as potential source for histone deacetylase inhibitors and epigenetic modulation

Isolation and Bioprocess Optimization of Halophilic and Alkaline Protease From Marine Streptomyces and Its Use As Contact Lens Cleaner

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