Screening and characterization of an Agrobacterium tumefaciens mutant strain producing high level of coenzyme Q10

Kim, Tae Su; Yoo, Ji Hyun; Kim, Sang Yong; Pan, Cheol Ho; Kalia, Vipin Chandra; Lee, Jung-Kul

doi:10.1016/j.procbio.2014.10.024

Cited by 8 publications

(5 citation statements)

References 38 publications

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“…Random mutagenesis and selection with menadione and sodium azide as inhibitors of the respiratory system generated mutants that overcame the growth inhibition with increased CoQ 10 production. Thus, titers up to 350 mg/L were reached in pH–stat fed-batch fermentations using these classically obtained mutants (Kim et al 2015 ). A. tumefaciens was used for two up-scaling steps (300 L and 5000 L) and produced CoQ 10 to a cellular content of 8.54 mg/g DCW and a titer of 458 mg/L (Ha et al 2007a ).…”

Section: Strategies To Improve Ubiquinone-related Production In Micro...mentioning

confidence: 99%

Recent advances in the metabolic pathways and microbial production of coenzyme Q

Pierrel

Burgardt

Lee

et al. 2022

World J Microbiol Biotechnol

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Coenzyme Q (CoQ) serves as an electron carrier in aerobic respiration and has become an interesting target for biotechnological production due to its antioxidative effect and benefits in supplementation to patients with various diseases. Here, we review discovery of the pathway with a particular focus on its superstructuration and regulation, and we summarize the metabolic engineering strategies for overproduction of CoQ by microorganisms. Studies in model microorganisms elucidated the details of CoQ biosynthesis and revealed the existence of multiprotein complexes composed of several enzymes that catalyze consecutive reactions in the CoQ pathways of Saccharomyces cerevisiae and Escherichia coli. Recent findings indicate that the identity and the total number of proteins involved in CoQ biosynthesis vary between species, which raises interesting questions about the evolution of the pathway and could provide opportunities for easier engineering of CoQ production. For the biotechnological production, so far only microorganisms have been used that naturally synthesize CoQ10 or a related CoQ species. CoQ biosynthesis requires the aromatic precursor 4-hydroxybenzoic acid and the prenyl side chain that defines the CoQ species. Up to now, metabolic engineering strategies concentrated on the overproduction of the prenyl side chain as well as fine-tuning the expression of ubi genes from the ubiquinone modification pathway, resulting in high CoQ yields. With expanding knowledge about CoQ biosynthesis and exploration of new strategies for strain engineering, microbial CoQ production is expected to improve.

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Section: Strategies To Improve Ubiquinone-related Production In Micro...mentioning

confidence: 99%

Recent advances in the metabolic pathways and microbial production of coenzyme Q

Pierrel

Burgardt

Lee

et al. 2022

World J Microbiol Biotechnol

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“…Neither A. tumefaciens nor R. sphaeroides produce sufficient quantities of CoQ 10 to meet current market demands, and this has led to higher prices of CoQ 10 [ 38 ]. Furthermore, rather than optimizing the hosts, recent efforts in the field have been to develop toolkit pieces, such as promoter-regulated vectors [ 98 , 99 ], or to determine ways to select for particular strains after mutagenesis [ 105 ]; only a few studies have attempted to harness metabolic engineering (to increase gene expression) or protein engineering [ 83 , 87 ]. Other efforts garnered toward a more immediate solution have had to rely on the addition of precursors to increase yield, and this comes at a higher cost and therefore remains less feasible [ 106 , 107 ].…”

Section: Introductionmentioning

confidence: 99%

Cellular factories for coenzyme Q10 production

et al. 2017

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Coenzyme Q10 (CoQ10), a benzoquinone present in most organisms, plays an important role in the electron-transport chain, and its deficiency is associated with various neuropathies and muscular disorders. CoQ10 is the only lipid-soluble antioxidant found in humans, and for this, it is gaining popularity in the cosmetic and healthcare industries. To meet the growing demand for CoQ10, there has been considerable interest in ways to enhance its production, the most effective of which remains microbial fermentation. Previous attempts to increase CoQ10 production to an industrial scale have thus far conformed to the strategies used in typical metabolic engineering endeavors. However, the emergence of new tools in the expanding field of synthetic biology has provided a suite of possibilities that extend beyond the traditional modes of metabolic engineering. In this review, we cover the various strategies currently undertaken to upscale CoQ10 production, and discuss some of the potential novel areas for future research.

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“…Moreover, this approach is attractive to the industry because the process is easy to control at a relatively low production cost (Tokdar et al, 2014). However, due to the limits of CoQ10 accumulation in cells, strain improvements have been made using genetic engineering (using recombinant nucleic acid technology), chemical mutagenesis and high hydrostatic pressure treatment (Kim et al, 2015).…”

Section: Microbial Sources Of Coq10mentioning

confidence: 99%

Coenzyme Q10 and its Effective Sources

Vaghari¹,

Vaghari²,

Jafarizadeh‐Malmiri³

et al. 2016

American Journal of Biochemistry and Biotechnology

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Coenzyme Q10 (2,3-dimethoxy, 5-methyl, 6-decaprenyl benzoquinone, CoQ10) is naturally present in many organisms. It has key roles in several biochemical pathways. CoQ10, as an electron and proton carrier for energy coupling leads to Adenosine Triphosphate (ATP) formation. Furthermore, in medicine, the pharmacological use of CoQ10 has attracted more attention due to its benefits in treating cardiovascular and degenerative neurologic diseases. CoQ10 can be produced by chemical synthesis, extraction from biological tissues and microbial fermentation. It is found in plants such as soya bean, peanut, palm oil and litchi pericarp and in animals such as pelagic fish, beef and pork hearts. Various analytical methods have been published for the extraction and analysis of CoQ10 from different matrices. Biological production of CoQ10 offers an environmentally benign option based on the enzymatic catalysis at the cellular level. Moreover, this process due to ease of control and low production costs offers more advantages over the existing technologies.

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Screening and characterization of an Agrobacterium tumefaciens mutant strain producing high level of coenzyme Q10

Cited by 8 publications

References 38 publications

Recent advances in the metabolic pathways and microbial production of coenzyme Q

Recent advances in the metabolic pathways and microbial production of coenzyme Q

Cellular factories for coenzyme Q10 production

Coenzyme Q10 and its Effective Sources

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