Screening and Antiviral Analysis of Phages That Display Peptides with an Affinity to Subunit C of Porcine Aminopeptidase

Guo, Donghua; Zhu, Qinghe; Feng, Li; Sun, Dongbo

doi:10.1089/mab.2013.0038

Cited by 3 publications

(2 citation statements)

References 19 publications

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“…Sun et al [ 101 ] reported construction and evaluation of a murine scFv library against the common receptor porcine aminopeptidase N (pAPN) of porcine coronaviruses TGEV and PEDV using the T7 phage display system [ 101 ]. Using recombinant rpAPN-C subunit and applying epitope mapping lead to identification of a peptide sequence with a potential role as a small molecular therapeutic agent against TGEV infection [ 102 ]. Identification of Neural Cell Adhesion Molecule (NCAM) as a novel interacting partner of the porcine hemagglutinating encephalomyelitis virus (PHE-CoV) using its S protein for biopanning a T7 phage display cDNA library was performed [ 103 ].…”

Section: Antibody Selection Against Coronaviruses Using Phage Displaymentioning

confidence: 99%

Phage Display Technique as a Tool for Diagnosis and Antibody Selection for Coronaviruses

et al. 2021

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Phage display is one of the important and effective molecular biology techniques and has remained indispensable for research community since its discovery in the year 1985. As a large number of nucleotide fragments may be cloned into the phage genome, a phage library may harbour millions or sometimes billions of unique and distinctive displayed peptide ligands. The ligand–receptor interactions forming the basis of phage display have been well utilized in epitope mapping and antigen presentation on the surface of bacteriophages for screening novel vaccine candidates by using affinity selection-based strategy called biopanning. This versatile technique has been modified tremendously over last three decades, leading to generation of different platforms for combinatorial peptide display. The translation of new diagnostic tools thus developed has been used in situations arising due to pathogenic microbes, including bacteria and deadly viruses, such as Zika, Ebola, Hendra, Nipah, Hanta, MERS and SARS. In the current situation of pandemic of Coronavirus disease (COVID-19), a search for neutralizing antibodies is motivating the researchers to find therapeutic candidates against novel SARS-CoV-2. As phage display is an important technique for antibody selection, this review presents a concise summary of the very recent applications of phage display technique with a special reference to progress in diagnostics and therapeutics for coronavirus diseases. Hopefully, this technique can complement studies on host–pathogen interactions and assist novel strategies of drug discovery for coronaviruses.

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Section: Antibody Selection Against Coronaviruses Using Phage Displaymentioning

confidence: 99%

Phage Display Technique as a Tool for Diagnosis and Antibody Selection for Coronaviruses

et al. 2021

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“…The PEDV belongs to the genus Alphacoronavirus, in the family Coronaviridae and order Nidovirales (Belouzard et al, 2012). Previous studies have investigated various control measures to protect against PEDV infection, such as vaccines, diagnostic tools, and therapeutic drugs (Sun et al, 2008;Ren et al, 2011;Sun et al, 2012;Zhu et al, 2013;Guo et al, 2013;Kim and Lee, 2013). Various aspects of PEDV infection remain unclear, for example, swine testis (ST) cells expressing porcine aminopeptidase N of PEDV receptor were not susceptible to PEDV infection.…”

Section: Introductionmentioning

confidence: 99%

Analysis of protein expression changes of the Vero E6 cells infected with classic PEDV strain CV777 by using quantitative proteomic technique

Sun

Shi

Guo

et al. 2015

Journal of Virological Methods

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Recent outbreaks of porcine epidemic diarrhea virus (PEDV) have caused widespread concern. The identification of proteins associated with PEDV infection might provide insight into PEDV pathogenesis and facilitate the development of novel antiviral strategies. We analyzed the differential protein profile of PEDV-infected Vero E6 cells using mass spectrometry and an isobaric tag for relative and absolute quantification. A total of 126 proteins were identified that were differentially expressed between the PEDV-infected and mock-infected groups (P<0.05, quantitative ratio ≥1.2), among which the expression of 58 proteins was up-regulated and that of 68 proteins was down-regulated in the PEDV-infected Vero E6 cells, involving in integrin β2/β3, cystatin-C. The Gene Ontology analysis indicated that the molecular function of the differentially expressed proteins (DEPs) was primarily related to binding and catalytic activity, and that the biological functions in which the DEPs are involved included metabolism, organismal systems, cellular processes, genetic information processing, environmental information processing, and diseases. Among the disease-related functions, certain anti-viral pathways and proteins, such as the RIG-I-like receptor, Rap1, autophagy, mitogen-activated protein kinase, PI3K-Akt and Jak-STAT signaling pathways, and integrin β2/β3 and cystatin-C proteins, represented potential factors in PEDV infection. Our findings provide valuable insight into PEDV-Vero E6 cell interactions.

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Antiviral peptides against Coronaviridae family: A review

et al. 2021

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Screening and Antiviral Analysis of Phages That Display Peptides with an Affinity to Subunit C of Porcine Aminopeptidase

Cited by 3 publications

References 19 publications

Phage Display Technique as a Tool for Diagnosis and Antibody Selection for Coronaviruses

Phage Display Technique as a Tool for Diagnosis and Antibody Selection for Coronaviruses

Analysis of protein expression changes of the Vero E6 cells infected with classic PEDV strain CV777 by using quantitative proteomic technique

Antiviral peptides against Coronaviridae family: A review

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