Analysis of protein expression changes of the Vero E6 cells infected with classic PEDV strain CV777 by using quantitative proteomic technique

Abstract

Recent outbreaks of porcine epidemic diarrhea virus (PEDV) have caused widespread concern. The identification of proteins associated with PEDV infection might provide insight into PEDV pathogenesis and facilitate the development of novel antiviral strategies. We analyzed the differential protein profile of PEDV-infected Vero E6 cells using mass spectrometry and an isobaric tag for relative and absolute quantification. A total of 126 proteins were identified that were differentially expressed between the PEDV-infected and mock-infected groups (P<0.05, quantitative ratio ≥1.2), among which the expression of 58 proteins was up-regulated and that of 68 proteins was down-regulated in the PEDV-infected Vero E6 cells, involving in integrin β2/β3, cystatin-C. The Gene Ontology analysis indicated that the molecular function of the differentially expressed proteins (DEPs) was primarily related to binding and catalytic activity, and that the biological functions in which the DEPs are involved included metabolism, organismal systems, cellular processes, genetic information processing, environmental information processing, and diseases. Among the disease-related functions, certain anti-viral pathways and proteins, such as the RIG-I-like receptor, Rap1, autophagy, mitogen-activated protein kinase, PI3K-Akt and Jak-STAT signaling pathways, and integrin β2/β3 and cystatin-C proteins, represented potential factors in PEDV infection. Our findings provide valuable insight into PEDV-Vero E6 cell interactions.

IL-22 suppresses the infection of porcine enteric coronaviruses and rotavirus by activating STAT3 signal pathway

“…The Vero cell-adapted PEDV CV777 strain, kindly provided by Maurice Pensaert at Ghent University (Merelbeke, Belgium), was propagated as previously described (Hofmann and Wyler, 1988;Sun et al, 2015). Briefly, Vero cells were inoculated with the virus at a multiplicity of infection (MOI) of 1 and cultured in serum-free DMEM for 72 h at 37 C with 5% CO 2 .…”

Cellular protein GLTSCR2: A valuable target for the development of broad-spectrum antivirals

“…Enterovirus A71 (EV-A71) strain BC08 , avian influenza virus (AIV) strain H9-WD (Wang et al, 2016b), canine distemper virus (CDV) strain MD77 (Lan et al, 2005), Newcastle disease virus (NDV) strain Lasota and infectious bronchitis virus (IBV) strain M41 (Li et al, 2013), porcine epidemic diarrhea virus (PEDV) strain CV777 (Sun et al, 2015), VSV strain Indiana (Wang et al, 2016a), HSV-1 strain F (Wang et al, 2011) and HCV strain JFH-1 (Xu et al, 2014) are reproduced in RD, MDCK, Vero, HeLa, Vero, HeLa, HEK293T, HeLa and Huh7.5.1 cells respectively, according to the reports indicated above. GFP-NDV strain NA-1 is generated by inserting an additional transcription cassette coding for the enhanced GFP between the P and M genes of the NDV genome (Wang et al, 2015).…”

Porcine Epidemic Diarrhea Virus Induces Autophagy to Benefit Its Replication

“…A previous proteomic study indicated that more differentially expressed proteins were mapped to the autophagy pathway, and the microtubule-associated protein 1B, a useful biomarker protein for autophagy was up-regulated in PEDV-infected Vero cells [18]. In addition, our previous study demonstrated that mTOR (the mammalian target of rapamycin) pathway, which was closely associated with cellular autophagy, was down-regulated, and that the autophagy associated protein ATG5 was up-regulated during PEDV infection [19].…”