“…Briefly, 1 mL of the previously withdrawn solutions for the release profile was added to 10 mm diameter holes cut into agar gel containing gram negative bacteria (Escherichia coli ATCC 25922) or gram positive bacteria ( Enterococcus faecalis ATCC 29212) at a bacterial concentration of 10 8 cells/mL. Plates were then incubated for 24 h at 37 °C [62] .…”