Screening and antimicrobial activity of actinomycetes isolated from the rhizosphere of Clitoria ternatea

Abstract

The air dried rhizosphere soil samples pretreated with calcium carbonate was employed for the isolation of actinomycete strains. Serial dilution plate technique was used for the isolation of actinomycetes. A total of 20 actinomycete strains designated as BS1-BS20 were isolated from the rhizosphere of medicinal plant Clitoria ternatea. All the 20 strains were subjected to primary screening for antimicrobial activity. Among the 20 strains screened, 10 strains exhibited high antimicrobial spectrum against Staphylococcus aureus, Escherichia coli and Candida albicans.

New biodegradable nanoparticles-in-nanofibers based membranes for guided periodontal tissue and bone regeneration with enhanced antibacterial activity

“…Briefly, 1 mL of the previously withdrawn solutions for the release profile was added to 10 mm diameter holes cut into agar gel containing gram negative bacteria (Escherichia coli ATCC 25922) or gram positive bacteria ( Enterococcus faecalis ATCC 29212) at a bacterial concentration of 10 8 cells/mL. Plates were then incubated for 24 h at 37 °C [62] .…”