Screening and analyzing the potential bioactive components from Shaofu Zhuyu decoction, using human umbilical vein endothelial cell extraction and high‐performance liquid chromatography coupled with mass spectrometry

Abstract

In this paper, a useful method for screening and analyzing the potential bioactive components in bioassay-guided fraction (SF-11) from Shaofu Zhuyu decoction was developed using human umbilical vein endothelial cell (HUVEC) extraction and high-performance liquid chromatography coupled with Q-TOF/MS spectrometry. In addition, the protective effects on HUVEC damage induced by adrenaline in vitro were also investigated. The results showed that SF-11 significantly inhibited the endothelin (ET) release and reversed the NO secretion of HUVEC (p < 0.05), and promoted the PGI(2) release of HUVEC (p < 0.05). Two effective components, paeoniflorin and typhaneoside, from SF-11 were screened and identified using live cell extract and HPLC coupled with Q-TOF/MS spectrometry. The compounds, paeoniflorin and typhaneoside, showed significantly inhibiting effects on the ET release and reversing of NO secretion of HUVEC (p < 0.05), with similar effects to SF-11, and promoting the PGI(2) release of HUVEC at the concentration of 0.208 and 0.013 micromol/mL, respectively (p < 0.05). These data indicated that the method of live cell extraction coupled with HPLC-MS technology is feasible, rapid and useful for screening and analyzing potential bioactive components from TCMs.

Ultra-performance liquid chromatography–tandem mass spectrometry analysis of the bioactive components and their metabolites of Shaofu Zhuyu decoction active extract in rat plasma

“…The final filtrates were separated by gradient elution with different concentrations of ethanol (10-90%) from macroporous adsorptive resins and the different fractions were obtained. The active fraction (SF-11), 40% ethanol elution fraction, was obtained by evaluating its efficacy in treating dysmenorrhea [3][4][5][6][7][8]. The active fraction was dissolved in 5 mL methanol for UPLC-MS/MS analysis.…”

“…The activity and active components of SFZYD volatile oil have also been investigated [5]. The bioassay-guided separation of an aqueous extract of SFZYD led to the isolation of an active fraction, SF-11 which had obvious multiple activities of inhibition on isolated uterine contractions induced by Oxytocin, inhibition of platelet aggregation induced by ADP and PAF, and protection against HUVE cell damage induced by H 2 O 2 [2,6].…”

Simultaneous determination of twelve bioactive constituents in Buyang Huanwu decoction by HPLC‐DAD‐ELSD and HPLC‐TOF/MS

“…The methods coupled on-line, such as HPLC-DAD-ELSD (Qi et al, 2006), possess the advantages of complementarities and cost-effectiveness, and seem to be highly suited for routine analysis and quality evaluation. LC-MS methods, on the other hand, can achieve high sensitivity and provide more information on the constituents of interest (Cao et al, 2008;Su et al, 2008;Yang et al, 2007;Wei et al, 2007;Zhou et al, 2008). Therefore, a comprehensive analytical method involving both HPLC-DAD-ELSD and LC-MS would appear to be a good approach to analyzing the bioactive components of herbal prescription and their products.…”

Antioxidant capacity of Typha angustifolia extracts and two active flavonoids

“…According to the pharmacological effects of these compounds, it has been suggested that flavonoids may play a major role as an antioxidant (Bouhlel et al 2009(Bouhlel et al , 2010. Besides, the constituents in the pollen have diverse biological effects, such as analgesic activity (Ma et al 2011), inhibition of the contractile activity of isolated uterus (Su et al 2010), and protection of the human umbilical vein endothelial cells against adrenaline-induced damage (Su et al 2008).…”