Antioxidant capacity of Typha angustifolia extracts and two active flavonoids

Abstract

Context: The pollen of Typha angustifolia L. (Typhaceae) has been used as a traditional Chinese medicine for improving the microcirculation and promoting wound healing. Flavonoids are the main constituent in the plant, but little is known about the antioxidant activity of the principal constituent of the pollen in detail. Objectives: To assess the antioxidant activities of ethanol and water extracts and two constituents of the pollen. Materials and methods: Plant material (1 g) was extracted by 95% ethanol and water (10 mL Â 2, 1 h each), respectively. The extracted activities (0.8-2.6 mg/mL) were measured by DPPH and the reducing activity of ferric chloride (1.7-2.6 mg/mL). Typhaneoside and isorhamnetin-3-O-neohesperidoside (I3ON) (2.8-70 lmol/L) were investigated on the relationship between NO, MDA and SOD in HUVECs treated with 100 lg/mL of LPS for 24 h. Results: Nine compounds were identified by UPLC-MS. Ethanol extract showed IC 50 values in DPPH (39.51 ± 0.72) and Fe 3þ reducing activity (82.76 ± 13.38), higher than the water extract (50.85 ± 0.74) and (106.33 ± 6.35), respectively. Typhaneoside and I3ON promoted cell proliferation at the respective concentration range of 2.8 to 70 lmol/L (p < 0.01). This two compounds decreased MDA (1.91 ± 0.10, 1.80 ± 0.34, p < 0.05) and NO levels (14.64 ± 0.08, 13.10 ± 0.88, p < 0.01), respectively, and increased SOD level (22.94 ± 2.48, 23.57 ± 2.38, p < 0.01) at the concentration of 70 lmol/L compared with LPS group. Conclusions: The constituents from Typha angustifolia could be a novel therapeutic strategy for LPSinduced inflammation. ARTICLE HISTORY

Citrus aurantium Naringenin Prevents Osteosarcoma Progression and Recurrence in the Patients Who Underwent Osteosarcoma Surgery by Improving Antioxidant Capability

“…C. aurantium is rich in flavonoids [ 18 , 19 ], which have antioxidants [ 20 , 21 ] and anti-inflammation properties [ 22 ]. Reactive oxygen species (ROS) are closely associated with oxidative stress, which is ubiquitous in a biological system.…”

Protective role of Pollen Typhae total flavone against the palmitic acid-induced impairment of glucose-stimulated insulin secretion involving GPR40 signaling in INS-1 cells

“…Moreover, kaempferol was found to protect INS-1E cells and human islets against palmitate-induced apoptosis and improve palmitate-induced dysfunction via upregulation of the PDX-1/cAMP/PKA/CREB signaling cascade (50). It was confirmed that increased FFAs induce oxidative stress to impair GSIS in pancreatic islets (42,43), and typhaneoside and isorhamnetin-3-O-neohesperidoside have antioxidant capacity against lipopolysaccharide in human umbilical vein endothelial cells (12). These two compounds may mediate insulin secretion in pancreatic β cells.…”

“…We previously reported that PTF contains typhaneoside and other components (16). Moreover, Pollen Typhae extract was further determined to contain several flavonoids including typhaneoside, isorhamnetin, naringenin, kaempferol, quercetin and isorhamnetin-3-O-neohesperidoside (12,13,48). Studies indicate that naringenin and quercetin enhance GSIS in INS-1E cells (49), while kaempferol improves the impairment of GSIS induced by palmitate in INS-1E cells and human islets (50), which are in line with the findings of the present study.…”

“…Pollen Typhae, a Chinese herbal medicine, contains flavonoids such as naringenin, kaempferol, isorhamnetin, isorhamnetin-3-O-neohesperidoside, typhaneoside, and other constituents (12,13). According to previous reports, Pollen Typhae has anticoagulant activity (14) and functions to treat trauma, coronary heart diseases and myocardial infarction (15).…”

Discovery of bioactive compounds in the Chinese herbal formula NaoShuanTong Capsule (NSTC) against hemorheological disorders

“…Typhaneoside, isorhamnetin-3-Oneohesperidoside and benzoyl-oxypaeoniflorin were associated with more than one effect and could thus be regarded as the core bioactive compounds against hemorheological disorders. It has been reported that isorhamnetin-3-O-neohesperidoside and typhaneoside have close association with antioxidant capacity [20]. Benzoyl-oxypaeoniflorin might have anti-inflammatory and anti-oxidant effects [21].…”