Screening active fractions from Pinus massoniana pollen for inhibiting ALV-J replication and their structure activity relationship investigation

Cui, Wenping; Huang, Jin; Niu, Xiangyun; Shang, Hongqi; Zhou, Sha; Miao, Yongqiang; Wang, Huan; Chen, Ruichang; Wei, Kai; Zhu, Rui‐Liang

doi:10.1016/j.vetmic.2020.108908

Cited by 10 publications

(4 citation statements)

References 32 publications

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“…Following necropsy, the major avian immune organ (bursa offabricius,spleen, thymus) were sampled and weighed to determine the immune organs' relative weights. At 1, 3, 5, 8, 11 and 15 wpi, viremia was determined in 10 birds from each group, blood samples were obtained for virus isolation, and plasma was separated as described previously [ 16 ]. Briefly, the plasma was inoculated into the DF-1 cells grown in a 96-well plate and then the cultures were grown for 7 d before they were used for ALV-P27 antigen detection with an ELISA kit (Biochek, Holland).…”

Section: Methodsmentioning

confidence: 99%

The coinfection of ALVs causes severe pathogenicity in Three-Yellow chickens

Wang,

Wang

et al. 2024

BMC Vet Res

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The coinfection of ALVs (ALV-J plus ALV-A or/and ALV-B) has played an important role in the incidence of tumors recently found in China in local breeds of yellow chickens. The study aims to obtain a better knowledge of the function and relevance of ALV coinfection in the clinical disease of avian leukosis, as well as its unique effect on the pathogenicity in Three-yellow chickens. One-day-old Three-yellow chicks (one day old) were infected with ALV-A, ALV-B, and ALV-J mono-infections, as well as ALV-A + J, ALV-B + J, and ALV-A + B + J coinfections, via intraperitoneal injection, and the chicks were then grown in isolators until they were 15 weeks old. The parameters, including the suppression of body weight gain, immune organ weight, viremia, histopathological changes and tumor incidence, were observed and compared with those of the uninfected control birds. The results demonstrated that coinfection with ALVs could induce more serious suppression of body weight gain (P < 0.05), damage to immune organs (P < 0.05) and higher tumor incidences than monoinfection, with triple infection producing the highest pathogenicity. The emergence of visible tumors and viremia occurred faster in the coinfected birds than in the monoinfected birds. These findings demonstrated that ALV coinfection resulted in considerably severe pathogenic and immunosuppressive consequences.

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Section: Methodsmentioning

confidence: 99%

The coinfection of ALVs causes severe pathogenicity in Three-Yellow chickens

Wang,

Wang

et al. 2024

BMC Vet Res

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“…The lentinan had a good antitumor activity with triple-helical stereo-configuration, but when the stereo-configuration was destroyed, the anti-tumor activity disappeared as consequence ( 58 ). The antiviral activity could be affected by the change of triple-helical structure of pollen polysaccharides in Pinus massoniana ( 59 ), which might be related to the chain rigidity and exposure sites of the triple-helical structure. Furthermore, the polysaccharide chain with triple-helical conformation is more rigid and easier to be recognized by the receptor ( 60 ).…”

Section: Structure-activity Relationshipmentioning

confidence: 99%

Structural Elucidation, Modification, and Structure-Activity Relationship of Polysaccharides in Chinese Herbs: A Review

et al. 2022

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Chinese herbal polysaccharides (CHPs) are natural polymers composed of monosaccharides, which are widely found in Chinese herbs and work as one of the important active ingredients. Its biological activity is attributed to its complex chemical structure with diverse spatial conformations. However, the structural elucidation is the foundation but a bottleneck problem because the majority of CHPs are heteropolysaccharides with more complex structures. Similarly, the studies on the relationship between structure and function of CHPs are even more scarce. Therefore, this review summarizes the structure-activity relationship of CHPs. Meanwhile, we reviewed the structural elucidation strategies and some new progress especially in the advanced structural analysis methods. The characteristics and applicable scopes of various methods are compared to provide reference for selecting the most efficient method and developing new hyphenated techniques. Additionally, the principle structural modification methods of CHPs and their effects on activity are summarized. The shortcomings, potential breakthroughs, and developing directions of the study of CHPs are discussed. We hope to provide a reference for further research and promote the application of CHPs.

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“…2A and B). Given the potential PPPS cytotoxicity to most normal cells reported in previous studies, 14,18 we chose two safe and effective PPPS concentrations, 400 μg mL −1 and 800 μg mL −1 , for the following assays in vitro.…”

Section: Ppps Inhibits the Cell Growth And Proliferation Of Hct-116 A...mentioning

confidence: 99%

Anti-tumor activity of polysaccharides extracted fromPinus massonianapollen in colorectal cancer-in vitroandin vivostudies

et al. 2022

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Screening active fractions from Pinus massoniana pollen for inhibiting ALV-J replication and their structure activity relationship investigation

Cited by 10 publications

References 32 publications

The coinfection of ALVs causes severe pathogenicity in Three-Yellow chickens

The coinfection of ALVs causes severe pathogenicity in Three-Yellow chickens

Structural Elucidation, Modification, and Structure-Activity Relationship of Polysaccharides in Chinese Herbs: A Review

Anti-tumor activity of polysaccharides extracted fromPinus massonianapollen in colorectal cancer-in vitroandin vivostudies

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