Screen-printed biosensors for the control of wine quality based on lactate and acetaldehyde determination

Avramescu, Alina; Noguer, Thierry; Avramescu, M.E.; Marty, Jean‐Louis

doi:10.1016/s0003-2670(01)01580-x

Cited by 76 publications

(41 citation statements)

References 14 publications

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“…Íàé÷àñò³øå äëÿ ðîçðîáêè ëàêòàòíèõ àìïå-ðîìåòðè÷íèõ á³îñåíñîð³â, ïðèçíà÷åíèõ äëÿ àíàë³ç³â âèíà, âèêîðèñòîâóþòüñÿ òðè ôåðìåí-òè -ëàêòàòäåã³äðîãåíàçà (ËÄ) [3,6,8,9], ëàê-òàòîêñèäàçà (ËOÄ) [4,5,7,10,11] àáî ôëàâîöè-òîõðîì b 2 (ÔÖ b 2 ) [12].…”

Section: âñòóïunclassified

Intact Recombinant Cells of the Yeast Hansenula Polymorpha, Over-Producing Formaldehyde Dehydrogenase, as the Sensitive Bioelements for Amperometric Assay of Formaldehyde

Paryzhak

Demkiv

Schuhmann³

et al. 2008

SEMST

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Section: âñòóïunclassified

Intact Recombinant Cells of the Yeast Hansenula Polymorpha, Over-Producing Formaldehyde Dehydrogenase, as the Sensitive Bioelements for Amperometric Assay of Formaldehyde

Paryzhak

Demkiv

Schuhmann³

et al. 2008

SEMST

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“…A estratégia de incorporação do MBRS mostrou-se bastante eficiente para a confecção dos eletrodos, uma vez que, nessa forma, foram evitadas perdas provocadas por desprendimentos da camada do mediador, provavelmente em virtude da diminuição da solubilidade do MB, o que ocasionou maior estabilidade de resposta. Vasilescu et al 27 , baseados em estudos anteriores 36,37 , cujo objetivo era a utilização do MB como modificador eletroquímico para sensores NAD + -dependentes, investigaram diversas formas de incorporação desse mediador em eletrodos serigrafados, seja adicionando diretamente o composto ao grafite, seja realizando sua polimerização eletroquímica, ou mesmo produzindo o MBRS antes da incorporação ao eletrodo de grafite. Os autores encontraram melhores respostas quando utilizaram o MB não-combinado, porém a proporção MBRS/grafite testada foi de 2:98 (m/m).…”

Section: Resposta Eletroquímica Dos Biossensoresunclassified

“…Apesar da elevada sensibilidade (LD = 1,5 μg L -1 ), o sensor apresentou reprodutibilidade bastante baixa. Posteriormente, diferentes sensores e técnicas de imobilização foram testados, com o objetivo de melhorar a estabilidade desses tipos de sensores, porém baseados em sistemas unienzimáticos [27][28][29]36,37 .…”

Section: Otimização Dos Teores Do Nad + Do Substrato E Da Enzima Aldhunclassified

Biossensor enzimático para detecção de fungicidas ditiocarbamatos: estudo cinético da enzima aldeído desidrogenase e otimização do biossensor

Lima¹,

Nunes²,

Noguer³

et al. 2007

Quím. Nova

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Recebido em 13/7/05; aceito em 3/4/06; publicado na web em 11/8/06 ENZYMATIC BIOSENSOR FOR THE DETECTION OF DITHIOCARBAMATE FUNGICIDES. KINETIC STUDY OF ALDEHYDE DEHYDROGENASE ENZYME AND BIOSENSOR OPTIMIZATION. Initially, all major factors that affect the rate of the AldH-catalyzed reaction (enzyme concentration, substrate concentration, temperature and pH) were investigated. Optimal activity was observed between pH values of 7.5 and 9.5 in the temperature range of 25 to 50 o C. Kinetic parameters, such as K m (2.92 μmol L -1 ) and V max (1.33 10 -2 μmol min -1 ) demonstrate a strong enzyme-substrate affinity. The sensors were based on screen-printed electrodes modified with the Meldola BlueReinecke salt (MBRS) combination. Operational conditions (NAD + and substrate contents, enzyme loading and response time) were optimized. Also, two enzyme immobilization procedures were tested: entrapment in poly(vinyl alcohol) bearing styrylpyridinium groups (PVA-SbQ) and crosslinking with glutaraldehyde. Chronoamperometry was employed to observe the biosensor responses during enzymatic hydrolysis of propionaldehyde and also to construct inhibition curves with maneb and zineb fungicides. Best results were found with the following conditions:; enzyme loading = 0.8 U per electrode; response time = 10 min, and inhibition time = 10 min. Current intensities around 103 ± 13 nA with the sensors and good stability was obtained for both immobilization procedures. Detection limits, calculated using 10% inhibition were 31.5 μg L -1 and 35 μg L -1 for maneb and zineb, respectively. Results obtained with other MBRS-modified electrodes consisting of mono and bi-enzymic sensors were compared. The ability to catalyze NADH oxidation by MB was also highlighted.Keywords: AldH-based biosensors; dithiocarbamate fungicides; enzymatic kinetic. INTRODUÇÃOA investigação dos pesticidas constitui um caso delicado. A necessidade de analisar resíduos de pesticidas depara-se com problemas analíticos complexos, devido à gama de substâncias existentes em classes distintas e que devem ser determinadas em diferentes tipos de matrizes, sejam elas ambientais ou de alimentos 1 . Entre os agrotóxicos mais difundidos atualmente, encontram-se os fungicidas ditiocarbamatos (DTC's). Tais compostos formam a mais importante classe de pesticidas empregados no controle de diversas doenças fungais em sementes, frutos e vegetais. Embora a toxidade aguda dos DTC's seja relativamente baixa, existe uma urgente necessidade de se encontrar um método de detecção sensível, confiável, e que permita rápidas tomadas de decisão, no caso de intoxicação em trabalhadores rurais e/ou contaminação ambiental ou alimentar. Isto devido ao fato de que, dependendo dos compostos encontrados, estes podem vir a ser carcinogênicos 2 , goitrogênicos 2 , mutagênicos 3-5 , teratogênicos 3,6,7 , provocar distúrbios neurológicos 8 e, também, afetar a biota aquática 9 . Há uma grande preocupação com relação a certos compostos como, por ex., os fungicidas maneb e ditane, que contêm manganês, precursor d...

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“…Based on the previous extensive studies, an essential principle of the development of SPE thick-film biosensors is enzyme immobilization [23,24]. Several methods for this have been investigated, for example, adsorption, cross linking, using solgel matrixes, and entrapment with polymer [25][26][27][28][29]. Despite these studies, there are many problems concerning simplification of fabrication, correct maintenance of enzymes on the biosensor surface, maintaining its activity, and ultimately increasing the life of the biosensors.…”

Section: Introductionmentioning

confidence: 99%

Amperometric Hydrogen Peroxide Biosensor Based on Horseradish Peroxidase Entrapped in Titania Sol-Gel Film on Screen-Printed Electrode

Sabzi¹,

Rasouli²,

Kheiri³

2013

AJAC

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We report the fabrication of disposable and flexible Screen-Printed Electrodes (SPEs). This new type of screen-printed electrochemical platform consists of Ag nanoparticles (AgNPs) and graphite composite. For this purpose, silver nanoparticles were first synthesized by a chemical reduction method. The morphology and structure of the AgNPs were analyzed using a Scanning Electron Microscope (SEM) and UV-Visible spectroscopy. Graphite was chosen as the working electrode material for the fabrication of a thick-film. The fabrication of a screen-printed hydrogen peroxide biosensor consisting of three electrodes on a polyethylene terephthalate (PET) substrate was performed with a spraying approach (working, counter and reference: enzyme electrode, graphite, pseudo reference: Ag/AgCl). This biosensor was fabricated by immobilizing the peroxidase enzyme (HRP) in a Titania sol-gel membrane which was obtained through a vapor deposition method. The biosensor had electrocatalytic activity in the reduction of H 2 O 2 with linear dependence on H 2 O 2 concentration in the range of 10 −5 to 10 −3 M; the detection limit was 4.5 × 10 −6 M.

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Screen-printed biosensors for the control of wine quality based on lactate and acetaldehyde determination

Cited by 76 publications

References 14 publications

Intact Recombinant Cells of the Yeast Hansenula Polymorpha, Over-Producing Formaldehyde Dehydrogenase, as the Sensitive Bioelements for Amperometric Assay of Formaldehyde

Intact Recombinant Cells of the Yeast Hansenula Polymorpha, Over-Producing Formaldehyde Dehydrogenase, as the Sensitive Bioelements for Amperometric Assay of Formaldehyde

Biossensor enzimático para detecção de fungicidas ditiocarbamatos: estudo cinético da enzima aldeído desidrogenase e otimização do biossensor

Amperometric Hydrogen Peroxide Biosensor Based on Horseradish Peroxidase Entrapped in Titania Sol-Gel Film on Screen-Printed Electrode

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