Amperometric Hydrogen Peroxide Biosensor Based on Horseradish Peroxidase Entrapped in Titania Sol-Gel Film on Screen-Printed Electrode

Abstract

We report the fabrication of disposable and flexible Screen-Printed Electrodes (SPEs). This new type of screen-printed electrochemical platform consists of Ag nanoparticles (AgNPs) and graphite composite. For this purpose, silver nanoparticles were first synthesized by a chemical reduction method. The morphology and structure of the AgNPs were analyzed using a Scanning Electron Microscope (SEM) and UV-Visible spectroscopy. Graphite was chosen as the working electrode material for the fabrication of a thick-film. The fabrication of a screen-printed hydrogen peroxide biosensor consisting of three electrodes on a polyethylene terephthalate (PET) substrate was performed with a spraying approach (working, counter and reference: enzyme electrode, graphite, pseudo reference: Ag/AgCl). This biosensor was fabricated by immobilizing the peroxidase enzyme (HRP) in a Titania sol-gel membrane which was obtained through a vapor deposition method. The biosensor had electrocatalytic activity in the reduction of H 2 O 2 with linear dependence on H 2 O 2 concentration in the range of 10 −5 to 10 −3 M; the detection limit was 4.5 × 10 −6 M.

A mediated turnip tissue paper-based amperometric hydrogen peroxide biosensor

“…Despite the simplicity of the present biosensor, it produced a lower LOD compared to other highly complex plant tissue-based H 2 O 2 biosensors fabricated with carbon paste matrices [29], nanorods [31] and nanoparticles [32]. Moreover, the linear range of this simple low-cost biosensor is comparable with other more complex H 2 O 2 biosensors [20,22,28,29,32]. …”

Amperometric Biosensor and Front-End Electronics for Remote Glucose Monitoring by Crosslinked PEDOT-Glucose Oxidase

“…The amperometric planar electrode platform used in the 161 present study consisted of the rectangular working electrode 162 (WE; 2 mm × 18 mm) placed in between quasi-reference 163 electrode (2 mm × 18 mm) and the L-shaped counter electrode 164 (CE; long arm: 22 mm; short arm: 7 mm; width: 2 mm) 165 deposited onto the flexible PET substrates [24]- [26]. The 166 dimensions were chosen so as to reach a compromise between 167 the fabrication method we exploited and the noise generated 168 by these electrodes.…”

A bimetallic nanocoral Au decorated with Pt nanoflowers (bio)sensor for H2O2 detection at low potential

“…In the last years the use of nanostructured-based modified electrodes able to drastically lower the applied potential gave new interest on the use of these biodevices [1,2]. In fact, although the first generation biosensors are still widely used, the principal drawback is that H 2 O 2 is sensed at a high potential (+0.70 V vs. Ag|AgCl) on most electrode materials and possible interfering components present in biological fluids such as ascorbic acid (AA), uric acid (UA) and acetaminophen (APAP) can be oxidized at this high potential and generate a faradic current that interferes in the measurement of the analyte [3,4].…”