Screen of Functional Activity of Polysaccharide and Glycosaminoglycan from Sea Hare (Aplysia kurodai) by Cell Line

Abstract

In this study, we extracted a whole polysaccharide fraction from the sea hare, Aplysia kurodai, and screened its functional properties using cell lines. The functionalities of polysaccharide and glycosaminoglycan (GAG) were investigated with RAW 264.7 cell lines. The crude polysaccharides and GAG purified DEAE-Sepharose chromatography did not show the toxicity on RAW 264.7 cell line in the range of 10～200 μg/mL, whereas they increased the cell growth rate. The crude polysaccharides and purified GAG also increased the production of NO, interleukin-6 and tumor necrosis factor-α on RAW 264.7 cell. Particularly, the purified GAG inhibited the proliferation of stomach cancer cell line, AGS, up to 40% for 72 hr incubation, but not the intestinal epithelial IEC-6 cell lines.

Aplysia kurodai -derived glycosaminoglycans increase the phagocytic ability of macrophages via the activation of AMP-activated protein kinase and cytoskeletal reorganization in RAW264.7 cells

“…Compared to mammals, sea hares are safer and good materials for massive production of GAGs. Although GAGs have been isolated and identified from the sea hare Aplysia kurodai (A. kurodai), an East-Asian species of marine gastropod (Hong, Park, Yoon, Choi, & Choi, 2011), little is known regarding the role of GAGs isolated from the sea hare A. kurodai in phagocytosis.…”