Extraction, structural characterization, and antioxidant and immunomodulatory activities of a polysaccharide from Notarchus leachii freeri eggs

Pan, Qiting; Sun, Yulin; Li, Xuyan; Zeng, Buyan; Chen, Daohai

doi:10.1016/j.bioorg.2021.105275

Cited by 15 publications

(9 citation statements)

References 64 publications

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“…37 The absorption peaks at 1160 cm −1 , 1075 cm −1 , and 1045 cm −1 could be responsible for the stretching vibration of the C–O bond on the pyranose ring. 38,39 Furthermore, the absorption peaks between 800 and 1000 cm −1 demonstrated that GAP-2 possessed both α- and β-configurations, 40 which was confirmed by NMR results. The above results indicated that GAP-2 showed the characteristic absorption peaks of polysaccharides.…”

Section: Resultssupporting

confidence: 62%

Structural features and in vitro antitumor activity of a water-extracted polysaccharide from Ganoderma applanatum

et al. 2023

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Section: Resultssupporting

confidence: 62%

Structural features and in vitro antitumor activity of a water-extracted polysaccharide from Ganoderma applanatum

et al. 2023

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“…NO is a gaseous molecule that enhances the lysis and phagocytosis of macrophages and is an excellent biomarker for evaluating macrophage activation [ 63 ]. TNF-α is the earliest mediator of inflammatory reactions and can promote the activity of macrophages [ 64 ]. IL-1β plays a vital role in macrophage activation and acts with TNF-α in inflammation [ 65 ].…”

Section: Discussionmentioning

confidence: 99%

Isolation, Structural Characterization and Macrophage Activation Activity of an Acidic Polysaccharide from Raspberry Pulp

et al. 2022

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The discovery of safe and effective plant polysaccharides with immunomodulatory effects has become a research hotspot. Raspberry is an essential commercial fruit and is widely distributed, cultivated, and consumed worldwide. In the present study, a homogeneous acidic polysaccharide (RPP-2a), with a weight-average molecular weight (Mw) of 55582 Da, was isolated from the pulp of raspberries through DEAE-Sepharose Fast Flow and Sephadex G-200 chromatography. RPP-2a consisted of rhamnose, arabinose, galactose, glucose, xylose, galacturonic acid and glucuronic acid, with a molar ratio of 15.4:9.6:7.6:3.2:9.1:54.3:0.8. The results of Fourier transform infrared spectroscopy (FT-IR), gas chromatography-mass spectrometer (GC-MS), 1D-, and 2D-nuclear magnetic resonance (NMR) analyses suggested that the backbone of RPP-2a was primarily composed of →2)-α-L-Rhap-(1→, →2,4)-α-L-Rhap-(1→, →4)-α-D-GalAp-(1→, and →3,4)-α-D-Glcp-(1→ sugar moieties, with side chains of α-L-Araf-(1→, α-L-Arap-(1→, and β-D-Galp-(1→3)-β-D-Galp-(1→ residues linked to the O-4 band of rhamnose and O-3 band of glucose residues. Furthermore, RPP-2a exhibited significant macrophage activation activity by increasing the production of nitric oxide (NO), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-1β (IL-1β), and the expression of inducible nitric oxide synthase (iNOS) and cytokines at the transcriptional level in RAW264.7 cells. Overall, the results indicate that RPP-2a can be utilized as a potential natural immune-enhancing agent.

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“…Thus, it is an excellent biomarker for evaluating macrophage activation [ 52 ]. TNF-α is the earliest and a potent pro-inflammatory mediator secreted by activated macrophages, which exerts a variety of biological effects, such as cell proliferation, differentiation, and multiple pro-inflammatory effects [ 53 ]. IL-1β acts with TNF-α in inflammatory processes and plays a vital role in the activation of macrophages [ 54 ].…”

Section: Discussionmentioning

confidence: 99%

“…The scavenging ability of hydroxyl radicals was determined by the previously reported Fenton method [ 53 ]. Briefly, 1 mL of polysaccharide solution was mixed with 1 mL of ferrous sulfate solution (9 mM), salicylic acid-ethanol solution (9 mM), and H 2 O 2 solution (8.8 mM) at various concentrations (0.05, 0.1, 0.2, 0.4, 0.8, 1.6 and 3.2 mg/mL).…”

Section: Methodsmentioning

confidence: 99%

Structural Characteristics, Antioxidant, and Immunostimulatory Activities of an Acidic Polysaccharide from Raspberry Pulp

et al. 2022

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The extraction and characterization of new bioactive plant-derived polysaccharides with the potential for use as functional foods and medicine have attracted much attention. In the present study, A novel acidic polysaccharide (RPP-3a) with a weight-average molecular weight (Mw) of 88,997 Da was isolated from the raspberry pulp. RPP-3a was composed of rhamnose, arabinose, galactose, glucose, mannose, and galacturonic acid at a molar ratio of 13.1:28.6:16.8:1.4:6.2:33.9. Structural analysis suggested that the RPP-3a backbone was composed of repeating units of →4)-β-Galp-(1→3,4)-α-Rhap-(1→[4)-α-GalAp-(1→4)-α-GalAp-(1→]n with branches at the C-4 position of rhamnose. The side chain of RPP-3a, containing two branch levels, was comprised of α-Araf-(1→, →5)-α-Araf-(1→, →3,5)-α-Araf-(1→, →3)-β-Galp-(1→, →3,6)-β-Galp-(1→, →4)-β-Glcp-(1→, and →2,6)-α-Manp-1→ residues. RPP-3a exhibited moderate reducing power and strong hydroxyl and superoxide anion radical scavenging abilities. RPP-3a significantly promoted the viability of RAW264.7 macrophages by increasing the production of nitric oxide (NO), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and interleukin-1β (IL-1β) at both the expression and transcriptional levels. In summary, the immunostimulatory and antioxidant activities make RPP-3a a viable candidate as a health-beneficial functional dietary supplement.

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Extraction, structural characterization, and antioxidant and immunomodulatory activities of a polysaccharide from Notarchus leachii freeri eggs

Cited by 15 publications

References 64 publications

Structural features and in vitro antitumor activity of a water-extracted polysaccharide from Ganoderma applanatum

Structural features and in vitro antitumor activity of a water-extracted polysaccharide from Ganoderma applanatum

Isolation, Structural Characterization and Macrophage Activation Activity of an Acidic Polysaccharide from Raspberry Pulp

Structural Characteristics, Antioxidant, and Immunostimulatory Activities of an Acidic Polysaccharide from Raspberry Pulp

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