α‐Asarone alleviates allergic asthma by stabilizing mast cells through inhibition of ERK/JAK2‐STAT3 pathway

Abstract

Asthma is a heterogeneous disease related to numerous inflammatory cells, among which mast cells play an important role in the early stages of asthma. Therefore, treatment of asthma targeting mast cells is of great research value. α‐Asarone is an important anti‐inflammatory component of the traditional Chinese medicine Acorus calamus L, which has a variety of medicinal values. To investigate whether α‐asarone can alleviate asthma symptoms and its mechanism. In this study, we investigated the effect of α‐asarone on mast cell activation in vivo and in vitro. The release of chemokines or cytokines, AHR (airway hyperresponsiveness), and mast cell activation were examined in a mast cell‐dependent asthma model. Western blot was performed to determine the underlying pathway. α‐Asarone inhibited the degranulation of LAD2 (laboratory allergic disease 2) cells and decreased IL‐8, MCP‐1, histamine, and TNF‐α in vitro. α‐Asarone reduced paw swelling and leakage of Evans blue, as well as serum histamine, CCL2, and TNF‐α in vivo. In the asthma model, α‐asarone showed an inhibitory effect on AHR, inflammation, mast cells activation, infiltration of inflammatory cells, and the release of IL‐5 and IL‐13 in lung tissue. α‐Asarone decreased the levels of phosphorylated JAK2, phosphorylated ERK, and phosphorylated STAT3 induced by C48/80. Our findings suggest that α‐asarone alleviates allergic asthma by inhibiting mast cell activation through the ERK/JAK2‐STAT3 pathway.

Biodegradable Mesoporous Organosilica-Based Nanostabilizer Targeting Mast Cells for Long-Term Treatment of Allergic Diseases

“…In addition, Evans blue exudation images on paws showed a consistent trend with dorsal skin tissue (Figure d,g). Typically, a certain degree of inflammatory reaction will happen along with allergic stimulation, which can induce the thickness of paws to increase . By measuring the thickness changes of paws before and after treatment, as shown in Figure f, the swelling of paws was reduced after the nanocarriers group.…”

“…Typically, a certain degree of inflammatory reaction will happen along with allergic stimulation, which can induce the thickness of paws to increase. 66 By measuring the thickness changes of paws before and after treatment, as shown in Figure 4f, the swelling of paws was reduced after the nanocarriers group. The thickness of the paws almost returned to a normal level after the treatment of Se MSNs@CS@Ap.…”

Icariin attenuates asthmatic airway inflammation via modulating alveolar macrophage activation based on network pharmacology and in vivo experiments

“…Up to now, several studies have shown that it promotes the symptoms of asthma. Jak2 mRNA and p‐JAK2 protein significantly increase following OVA or PM2.5 exposure to induce airway inflammation 65,85 . Shen et al 86 confirmed that airway smooth muscle cells (ASMCs) migration and proliferation are controlled by Jak2 in the pathogenesis of asthma.…”

“…Jak2 mRNA and p-JAK2 protein significantly increase following OVA or PM2.5 exposure to induce airway inflammation. 65,85 Shen et al 86 confirmed that airway smooth muscle cells (ASMCs) migration and proliferation are controlled by Jak2 in the pathogenesis of asthma. Recent studies have shown that the JAK2 signaling pathway has been demonstrated to be a keystone of M1 macrophage polarization.…”

“…The central to the airway inflammation process includes the recruitment of immune cells to the airway lumen and the abnormal expression of cytokines. 39,65 To confirm the effects of ICA on airway inflammation, counts of inflammatory cells in BALF were performed.…”