X. Yeast sequencing reports. Revised nucleotide sequence of the cor region of yeast Saccharomyces cerevisiae chromosome X

Abstract

The COR region, a gene cluster located on chromosome X of Saccharomyces cerevisiae and including genes CYC1, UTR1, UTR3, OSM1, tRNA(Gly) and RAD7, was sequenced within the framework of the European Union genome systematic sequencing project. It was compared with previously published sequences to be found in GenBank under the acronym YSCCORA. While some of the discrepancies observed can be readily ascribed to polymorphism, others most probably result from sequencing errors. A revised version of the sequence of the COR cluster is given.

Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.

“…When all bases had been determined by each contractor and each sequencing strategy had been approved by the DNA coordinator, ensuring that the sequence had been independently determined on each strand with sufficient overlap between all the subclones, the sequences were considered as final and entered into the MIPS data library for assembly. Partial sequences of chromosome X have been published independently by some of the authors of this work (Huang et al, 1994b(Huang et al, , 1995Miosga et al, 1994aMiosga et al, ,b,c, 1995Purnelle et al, 1994;Vandenbol et al, 1994Vandenbol et al, , 1995Rasmussen, 1995;Zagulski et al, 1995).…”

Analysis of a 42·5 kb DNA sequence of chromosome X reveals three tRNA genes and 14 new open reading frames including a gene most probably belonging to the family of ubiquitin–protein ligases

“…In contrast, another internal ORF, GTF173, with a size of 173 amino acids and a CAI of 0.14, might well be a real gene. Ten of the ORFs correspond to previously published S. cerevisiae sequences: PET191 (GTA108; McEwen el al., 1993); a yeast counterpart of the human helicase ERCC6 (GTA1085; Huang et al, 1994~); GEFl (GTC779; Greene et al, 1993), which was independently characterized by our group as a possible voltage-gated chloride channel (Huang et al, 1994d); SSCl (GTF654; Craig et al, 1989;Morishima et ul., 1990); TIFSlB (GTD157; Schnier et ul., 1991) and the five genes of the COR cluster (Melnick and Sherman, 1990;Huang et al, 1994a).…”