<scp>WT</scp>1 immunoenzyme staining using SurePath<sup>™</sup> processed urine cytology helps to detect kidney disease

Ohsaki, Hiroyuki; Sofue, Tadashi; Kawakami, Kimihiro; Nishijima, Yoko; Hara, Taiga; Matsunaga, Toru; Kushida, Yoshio; Haba, Reiji; Shigematsu, Yumie; Irino, Satoshi; Norimatsu, Yoshiaki

doi:10.1111/cyt.12227

Cited by 10 publications

(17 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Urine cytology slides were prepared following SurePath manual protocols . Briefly, 10 mL of fresh urine homogenised before dispensing was centrifuged in a conical tube at 600 g for 10 min.…”

Section: Methodsmentioning

confidence: 99%

“…Urine cytology slides were prepared following SurePath manual protocols. 6 Briefly, 10 mL of fresh urine homogenised before dispensing was centrifuged in a conical tube at 600 g for 10 min. The supernatant was discarded and the sediment resuspended in 5-10 mL of CytoRich Red TM (Becton Dickinson, Franklin Lakes, NJ, USA).…”

Section: Sample Preparationmentioning

confidence: 99%

“…Podocytes (glomerular epithelial cells) line the outside of glomerular capillaries and, thus, face Bowman's capsule and primary urine . Podocytes form part of the filtration barrier together with capillary endothelial cells and the glomerular basement membrane (GBM), to ensure selective permeability of the glomerular capillary wall . In glomerular injury or aging, podocytes retract and broaden their foot processes, and may detach from the GBM .…”

Section: Introductionmentioning

confidence: 99%

“…7,14,15,19 Previously, we reported the usefulness of urine cytology based on SurePath TM (Becton Dickinson, Franklin Lakes, NJ, USA) combined with immunoenzyme staining with an anti-WT1 antibody to identify urinary podocytes and active PECs; indeed, we found WT1 immunoenzyme staining of urine cytology could be used to detect some forms of kidney disease. 6 The purpose of this study was to assess the relationship between urinary WT1-positive cells (podocytes and active PECs) and renal biopsy WT-1 positive cells (podocytes, PECs and crescentic cells) to determine whether urinary WT1-positive cells are useful for detection of crescent formation.…”

mentioning

confidence: 99%

See 3 more Smart Citations

Urinary WT1‐positive cells as a non‐invasive biomarker of crescent formation

et al. 2017

Self Cite

View full text Add to dashboard Cite

The results of our study suggest urinary WT1-positive cells can be used to detect patients with crescent formation using 5 cells/10-mL cutoff value. WT1-positive glomerular podocytes and parietal epithelial cells may be shed into urine in active glomerular disease. This study, investigating the relationship between WT1-positive cells in urine and in the renal biopsy found no correlation; however, the results do suggest that, using a cutoff value of 5 cells/10 mL, WT1 positive urinary cells can be used to detect patients with crescent formation.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Sample Preparationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Urinary WT1‐positive cells as a non‐invasive biomarker of crescent formation

et al. 2017

Self Cite

View full text Add to dashboard Cite

show abstract

“…The WT1 protein has been used as a biomarker of podocyte loss from glomeruli in kidney disease [30, 31] and of the presence of damaged podocytes in urinary sediment [32, 33]. In this work, we clearly observed a reduction of podocytes in renal biopsies of children with NS and the presence of podocytes in the urine, but the reason for such podocyte detachment is not clear.…”

Section: Discussionmentioning

confidence: 64%

Cytoplasmic Localization of WT1 and Decrease of miRNA-16-1 in Nephrotic Syndrome

Zapata‐Benavides

Arellano-Rodríguez

Bollain-y-Goytia

et al. 2017

BioMed Research International

View full text Add to dashboard Cite

Nephrotic syndrome (NS) is a glomerular disease that is defined by the leakage of protein into the urine and is associated with hypoalbuminemia, hyperlipidemia, and edema. Steroid-resistant NS (SRNS) patients do not respond to treatment with corticosteroids and show decreased Wilms tumor 1 (WT1) expression in podocytes. Downregulation of WT1 has been shown to be affected by certain microRNAs (miRNAs). Twenty-one patients with idiopathic NS (68.75% were SSNS and 31.25% SRNS) and 10 healthy controls were enrolled in the study. Podocyte number and WT1 location were determined by immunofluorescence, and the serum levels of miR-15a, miR-16-1, and miR-193a were quantified by RT-qPCR. Low expression and delocalization of WT1 protein from the nucleus to the cytoplasm were found in kidney biopsies of patients with SRNS and both nuclear and cytoplasmic localization were found in steroid-sensitive NS (SSNS) patients. In sera from NS patients, low expression levels of miR-15a and miR-16-1 were found compared with healthy controls, but only the miR-16-1 expression levels showed statistically significant decrease (p = 0.019). The miR-193a expression levels only slightly increased in NS patients. We concluded that low expression and delocalization from the WT1 protein in NS patients contribute to loss of podocytes while modulation from WT1 protein is not associated with the miRNAs analyzed in sera from the patients.

show abstract

MicroRNAs in childhood nephrotic syndrome

Dhandapani

Venkatesan

Charmine

2021

Journal Cellular Physiology

View full text Add to dashboard Cite

The discovery of microRNAs (miRNAs) has opened up new avenues of research to understand the molecular basis of a number of diseases. Because of their conservative feature in evolution and important role in the physiological function, microRNAs could be treated as predictors for disease classification and clinical process based on the specific expression. The identification of novel miRNAs and their target genes can be considered as potential targets for novel drugs. Furthermore, currently, the circulatory and urinary exosomal miRNAs are gaining increasing attention as their expression profiles are often associated with specific diseases, and they exhibit great potential as noninvasive or minimally invasive biomarkers for the diagnosis of various diseases. The remarkable stability of these extracellular miRNAs circulating in the blood or excreted in the urine underscored their key importance as biomarkers of certain diseases. There is voluminous literature concerning the role of microRNAs in other diseases, such as cardiovascular diseases, diabetic nephropathy, and so forth. However, little is known about their diagnostic ability for the pediatric nephrotic syndrome (NS). The present review article highlights the recent advances in the role of miRNAs in the pathogenesis and molecular basis of NS with an aim to bring new insights into further research applications for the development of new therapeutic agents for NS.

show abstract

WT1 immunoenzyme staining using SurePath^™ processed urine cytology helps to detect kidney disease

Abstract: The results suggest that WT1 immunoenzyme staining of urine cytology can be used to detect some types of kidney disease.

Cited by 10 publications

References 25 publications

Urinary WT1‐positive cells as a non‐invasive biomarker of crescent formation

Urinary WT1‐positive cells as a non‐invasive biomarker of crescent formation

Cytoplasmic Localization of WT1 and Decrease of miRNA-16-1 in Nephrotic Syndrome

MicroRNAs in childhood nephrotic syndrome

Contact Info

Product

Resources

About

WT1 immunoenzyme staining using SurePath™ processed urine cytology helps to detect kidney disease

Abstract: The results suggest that WT1 immunoenzyme staining of urine cytology can be used to detect some types of kidney disease.

Cited by 10 publications

References 25 publications

Urinary WT1‐positive cells as a non‐invasive biomarker of crescent formation

Urinary WT1‐positive cells as a non‐invasive biomarker of crescent formation

Cytoplasmic Localization of WT1 and Decrease of miRNA-16-1 in Nephrotic Syndrome

MicroRNAs in childhood nephrotic syndrome

Contact Info

Product

Resources

About

WT1 immunoenzyme staining using SurePath^™ processed urine cytology helps to detect kidney disease