<scp>RUNX</scp>‐2, <scp>OPN</scp> and <scp>OCN</scp> expression induced by grey and white mineral trioxide aggregate in normal and hypertensive rats

Martins, Christine Men; Queiroz, Índia Olinta de Azevedo; Ervolino, Edílson; Cintra, Luciano Tavares Ângelo; Gomes‐Filho, João Eduardo

doi:10.1111/iej.12876

Cited by 18 publications

(19 citation statements)

References 28 publications

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“…The immunolabelling of OCN, OPN, and BSP for Bio‐C Pulpecto and MTA was low in the initial periods of analysis but was more intense from 30 days on. The data from this study are supported by previous studies, which revealed that calcium hydroxide‐releasing materials promote the expression of osteogenic markers also late . OCN is an important non‐collagen protein found in bone and dentin, which plays a role in the regulation of biomineralization and homeostasis of calcium ions .…”

Section: Discussionsupporting

confidence: 87%

“…OPN is an extracellular structural protein related to both bone formation/resorption, and it has been suggested that the OPN is involved in the host's defenses and also in the process of tissue repair . OCN and OPN are considered late markers of the biomineralization process . This explains why the immunolabelling for OCN and OPN was more intense from 30 days on.…”

Section: Discussionmentioning

confidence: 99%

“…The expression of bone markers in rat subcutaneous tissue induced by Bio‐C Pulpecto demonstrates its osteoinductivity. Bioceramic materials have been observed to induce the expression of bone markers at non‐osseous sites in animals without the addition of any osteogenic biomolecules . Osteoinductivity is considered an advantage since it is related to the formation of mineralized tissue during the healing process …”

Section: Discussionmentioning

confidence: 99%

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Biocompatibility and biomineralization ability of Bio‐C Pulpecto. A histological and immunohistochemical study

Cosme‐Silva

Benetti

Dal‐Fabbro

et al. 2019

Int J Paed Dentistry

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Aim To evaluate the inflammatory response, biomineralization and production of osteocalcin (OCN), osteopontin (OPN), and bone sialoprotein (BSP) of a new root filling material for primary teeth (Bio‐C Pulpecto) compared to MTA. Design Polyethylene tubes containing Bio‐C Pulpecto, MTA, and empty tubes were implanted into the dorsal connective tissue. After 7, 15, 30, 60, and 90 days, the tubes with surrounding tissue were removed and histologically processed to be analysed using haematoxylin and eosin, von Kossa staining, or no staining for observation under polarized light and immunohistochemistry for the detection of OCN, OPN, and BSP. The Kruskal‐Wallis test was used followed by Dunn's test. The significance level was set at 5%. Results The inflammatory response observed with MTA and Bio‐C Pulpecto was more exacerbated until the 15th day and decreased from 30 days on. No significant difference was found between control, MTA, and Bio‐C Pulpecto (P > 0.05). Bio‐C Pulpecto and MTA showed positivity for von Kossa and birefringent to polarized light. The immunolabelling for OCN, OPN, and BSP was more intense for MTA and Bio‐C Pulpecto on days 60 and 90 (P > 0.05). Conclusion Bio‐C Pulpecto was biocompatible, induced biomineralization and was immunopositive for osteogenic markers such as OCN, OPN, and BSP, similarly to MTA.

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Section: Discussionsupporting

confidence: 87%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Biocompatibility and biomineralization ability of Bio‐C Pulpecto. A histological and immunohistochemical study

Cosme‐Silva

Benetti

Dal‐Fabbro

et al. 2019

Int J Paed Dentistry

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“…, Martins et al . ) and considering an alpha error of 0.05 to recognize a significant difference, a minimum number of six rats/group was considered necessary. The animals were housed in rooms with controlled temperature and received water and food ad libitum .…”

Section: Methodsmentioning

confidence: 99%

“…A semiquantitative analysis of the immunolabelling was performed, and the immunolabelling pattern was based on a modification of the study of Martins et al . (): Score 0 – no immunolabelling (total absence of immunoreactive cells); Score 1 – extremely low immunolabelling; Score 2 – low immunolabelling; Score 3 – moderate immunolabelling; Score 4 – high immunolabelling; and Score 5 – extremely high immunolabelling.…”

Section: Methodsmentioning

confidence: 99%

Biocompatibility and immunohistochemical evaluation of a new calcium silicate‐based cement, Bio‐C Pulpo

Cosme‐Silva¹,

Gomes‐Filho²,

Benetti³

et al. 2019

Int Endodontic J

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Aim To evaluate the inflammatory response and ability to induce mineral deposition through histological and immunohistochemical analysis for osteocalcin (OCN), osteopontin (OPN) and bone sialoprotein (BSP) of a new calcium silicate‐based cement, Bio‐C Pulpo (Angelus), compared to white mineral trioxide aggregate (White MTA‐Ang) (Angelus). Methodology Polyethylene tubes containing Bio‐C Pulpo and White MTA‐Ang as well as empty tubes were implanted into the dorsal connective tissue of 30 Wistar rats, which were arranged in five groups according to the period of analysis: 7, 15, 30, 60 and 90 days. After each experimental period, the tubes with surrounding tissue were removed and histologically processed to be analysed using haematoxylin–eosin and immunohistochemistry for the detection of OCN, OPN and BSP. The data were statistically analysed (Friedman's test) at a 5% significance level. Results The inflammatory response observed with Bio‐C Pulpo and White MTA‐Ang was greater after 7 and 15 days and decreased from 30 days onwards. No significant difference was found between the control, Bio‐C Pulpo and White MTA‐Ang at the different periods of analysis (P > 0.05). The immunolabelling for OCN, OPN and BSP was more intense for Bio‐C Pulpo and White MTA‐Ang after 60 and 90 days, but there was no difference between Bio‐C Pulpo and White MTA‐Ang at the different periods of analysis (P > 0.05). Conclusion Bio‐C Pulpo is biocompatible and induces immunolabelling of osteogenic markers such as OCN, OPN and BSP similar to White MTA‐Ang.

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MicroRNA‐142 regulates osteoblast differentiation and apoptosis of mouse pre‐osteoblast cells by targeting bone morphogenetic protein 2

et al. 2020

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Here, we explored the effect of microRNA‐142 (miR‐142) on the viability of a mouse preosteoblast cell line. Cell viability was inhibited or promoted after transfection of miR‐142 mimic or inhibitor, respectively. Moreover, bone morphogenetic protein 2 (BMP2) is a target of miR‐142. Our results imply that miR‐142 might influence viability and differentiation of osteoblast cells by regulating BMP2 and BMP/Smad signaling.

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RUNX‐2, OPN and OCN expression induced by grey and white mineral trioxide aggregate in normal and hypertensive rats

Abstract: Hypertension decreased the immunostaining of RUNX-2, OPN and OCN biomarkers in response to MTA. Thus, hypertension can jeopardize the mineralization ability of MTA and may have a negative impact on endodontic treatment outcomes.

Cited by 18 publications

References 28 publications

Biocompatibility and biomineralization ability of Bio‐C Pulpecto. A histological and immunohistochemical study

Biocompatibility and biomineralization ability of Bio‐C Pulpecto. A histological and immunohistochemical study

Biocompatibility and immunohistochemical evaluation of a new calcium silicate‐based cement, Bio‐C Pulpo

MicroRNA‐142 regulates osteoblast differentiation and apoptosis of mouse pre‐osteoblast cells by targeting bone morphogenetic protein 2

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