Biocompatibility and biomineralization ability of Bio‐C Pulpecto. A histological and immunohistochemical study

Abstract

Aim To evaluate the inflammatory response, biomineralization and production of osteocalcin (OCN), osteopontin (OPN), and bone sialoprotein (BSP) of a new root filling material for primary teeth (Bio‐C Pulpecto) compared to MTA. Design Polyethylene tubes containing Bio‐C Pulpecto, MTA, and empty tubes were implanted into the dorsal connective tissue. After 7, 15, 30, 60, and 90 days, the tubes with surrounding tissue were removed and histologically processed to be analysed using haematoxylin and eosin, von Kossa staining, or no staining for observation under polarized light and immunohistochemistry for the detection of OCN, OPN, and BSP. The Kruskal‐Wallis test was used followed by Dunn's test. The significance level was set at 5%. Results The inflammatory response observed with MTA and Bio‐C Pulpecto was more exacerbated until the 15th day and decreased from 30 days on. No significant difference was found between control, MTA, and Bio‐C Pulpecto (P > 0.05). Bio‐C Pulpecto and MTA showed positivity for von Kossa and birefringent to polarized light. The immunolabelling for OCN, OPN, and BSP was more intense for MTA and Bio‐C Pulpecto on days 60 and 90 (P > 0.05). Conclusion Bio‐C Pulpecto was biocompatible, induced biomineralization and was immunopositive for osteogenic markers such as OCN, OPN, and BSP, similarly to MTA.

Inflammatory profile of apical periodontitis associated with liver fibrosis in rats: histological and immunohistochemical analysis

“…Scores were adapted from Cosme‐Silva et al . (2019a). Score 0: no immunolabelling (total absence of immunoreactive); Score 1: <20% – immunoreactive cells and low extracellular matrix labelling; Score 2: >20% and <40% – immunoreactive cells and low extracellular matrix labelling; Score 3: >40% and <60% – immunoreactive cells and moderate extracellular matrix labelling; Score 4: >60% and <80% – immunoreactive cells and high extracellular matrix labelling; Score 5: >80% – immunoreactive cells and high extracellular matrix labelling.…”

Influence of the Vehicle on the Tissue Reaction and Biomineralization of Fast Endodontic Cement

“…Thirty male rats (Wistar) weighing 250-280 g and 3 to 4 months of age were used in this study. The sample size was estimated based on data from a previous study [5] but using a power sample of 90%, which is corroborated by previous studies [3,5,17] and considering an alpha error of 0.05 to recognize a significant difference, a minimum number of six rats/group was considered necessary. The rats were housed in environments with temperatures between 22°C and 24°C with a controlled light cycle (12 hours light and 12 hours dark).…”

“…One hundred and twenty polyethylene tubes (Abbott Laboratórios do Brasil Ltda., São Paulo, SP, Brazil) with 1.0 mm internal diameter, 1.6 mm external diameter and 10.0 mm length were filled with the tested materials [3,5,17]. The Angelus MTA (Angelus Indústria de Produtos Odontológicos, Londrina, PR, Brazil) and experimental cement CER (School of Engineering, Department of Physics and Chemistry, Ilha Solteira, SP, Brazil) were prepared according to the manufacturer's recommendations (1 dose of MTA powder/1 drop of liquid; CER epoxy resin and CER water: 0.25 g of CER powder and 0.30 g of liquid) and inserted into the tubes.…”

“…The skin was reflected, creating 2 pockets in 1 side of the incision, one in the cranial portion and another in the caudal portion 6 cm away from each other, and another pocket on the opposite side of the incision. The tubes were implanted into the spaces created with blunt dissection, and the skin was closed with a 4/0 silk suture [3,5,17].…”

“…Histological sections were analysed under light-field illumination using optical microscope microscopy (400, DM 4000 B; Leica, Wetzlar, Germany) by a calibrated investigator and who was unaware of the experimental groups being analysed. The von Kossa technique was used to observe mineralized structures in the tissue, which were dark stained [5,17]. The polarized light technique enables observing birefringent structures related to calcium carbonate crystals originated from the combination of calcium ions from the material with carbonic gas from the tissue [5,17].…”